公开(公告)号：KR1020140014648A

公开(公告)日：2014-02-06

申请号：KR1020120081199

申请日：2012-07-25

Applicant: 삼성전자주식회사

Inventor： 이우용 ,  구현민 ,  김성수 ,  김재영 ,  박재찬

IPC: C12N1/21 ,  C12N15/52 ,  C12P7/16

CPC classification number: C12P7/18 ,  C07K14/34 ,  C08G63/16 ,  C08G63/183 ,  C12N9/1029 ,  C12N9/90 ,  C12N15/77 ,  C12P7/62

Abstract: The present invention relates to a modified microorganism for the highly efficient production of 1,4-butanediol, and a production method of 1,4-butanediol using the modified microorganism. The modified microorganism has improved tolerance against 1,4-butanediol by the loss or destruction of a gene encoding more than one selected from the group consisting of a transcriptional regulatory factor NCgl2886, GCN5-related N-acetyl transferase NCgl2090, hypothetical protein NCgl0224, and sugar phosphate isomerase/epimerase NCgl2956; and enables users to produce 1,4-butanediol in high efficiency even under the presence of the high concentration of 1,4-butanediol.

Abstract translation: 本发明涉及用于高效制备1,4-丁二醇的改性微生物，以及使用该改性微生物的1,4-丁二醇的制备方法。 修饰的微生物通过丧失或破坏编码多于一种的基因的转录调节因子NCgl2886，GCN5相关N-乙酰转移酶NCgl2090，假定蛋白NCgl0224和 糖磷酸异构酶/差向异构酶NCgl2956; 并且使用户即使在高浓度的1,4-丁二醇的存在下也能够高效地生产1,4-丁二醇。

公开(公告)号：KR1020110085668A

公开(公告)日：2011-07-27

申请号：KR1020100005576

申请日：2010-01-21

Applicant: 삼성전자주식회사 ,  고려대학교 산학협력단

Inventor： 조화영 ,  구현민 ,  박재찬 ,  박성민 ,  김경헌 ,  최인걸

IPC: C07K14/195 ,  C07K14/32 ,  C12P19/04 ,  C12N9/14

CPC classification number: C12P7/10 ,  C07K14/195 ,  C07K14/32 ,  C07K14/37 ,  C12N9/2437 ,  C12N9/248 ,  C12P19/02 ,  C12P19/14 ,  Y02E50/16 ,  Y02E50/343

Abstract: PURPOSE: An isolated protein and a composition containing the same for enhancing polysaccharide hydrolase activity are provided to enhance hydrolysis ability and to reduce the use of hydrolase. CONSTITUTION: An isolated protein enhances polysaccharide hydrolase activity and has polysaccharide binding ability. The protein contains GH45(glycoside hydrolase 45) or pollen-allergen domain. The polysaccharide hydrolase is cellulase, hemicellulase, alpha-amylase, glycoamylase, pectinase, xylanase, or cellobiase. The GH45 domain or pollen allergen domain is isolated from expansin, expansin-like protein, or bacterial protein.

Abstract translation: 目的：提供分离的蛋白质和含有该蛋白质的组合物，以增强多糖水解酶活性，以提高水解能力并减少水解酶的使用。 构成：分离蛋白提高多糖水解酶活性，具有多糖结合能力。 该蛋白质含有GH45（糖苷水解酶45）或花粉 - 变应原结构域。 多糖水解酶是纤维素酶，半纤维素酶，α-淀粉酶，糖酵解酶，果胶酶，木聚糖酶或纤维二糖酶。 从扩展蛋白，扩展蛋白样蛋白或细菌蛋白分离GH45结构域或花粉过敏原结构域。

公开(公告)号：KR1020140015998A

公开(公告)日：2014-02-07

申请号：KR1020120082816

申请日：2012-07-27

Applicant: 삼성전자주식회사 ,  한국과학기술원

Inventor： 이규상 ,  김태용 ,  손승범 ,  구현민 ,  박재찬 ,  이상엽

IPC: C12Q1/26 ,  G01N33/50 ,  C12N15/09 ,  C12P7/42

CPC classification number: G06F19/12 ,  C12P7/42

Abstract: An embodiment relates to a metabolic network model for analyzing metabolic characteristics of Kluyveromyces marxianus microbes for producing 3-hydroxypropionate (3HP) and to metabolic characteristic analyzation of the Kluyveromyces marxianus using the same. More specifically, the embodiment relates to construction of a metabolic network model of Kluyveromyces marxianus using gene-protein-biochemical response relationship, to analyzation of metabolic characteristic using the model such as metabolic engineering, and to a method for predicting a novel metabolic pathway which enhances productivity of 3HP using simulation based on the metabolic engineering. The method of the embodiment efficiently predicts productivity and cell growth speed of microbes, saves time and costs in order to optimize the novel path, and provides variant microorganisms which can provide specific metabolites.

Abstract translation: 一个实施方案涉及用于分析用于产生3-羟基丙酸酯（3HP）的马克斯克鲁维酵母微生物代谢特征的代谢网络模型和使用其的马克斯克鲁维酵母的代谢特征分析。 更具体地，本实施例涉及使用基因 - 蛋白质 - 生物化学反应关系构建马克斯克鲁维酵母的代谢网络模型，使用代谢工程等模型分析代谢特征，以及用于预测增强的新型代谢途径的方法 使用基于代谢工程的模拟，3HP的生产率。 本实施例的方法有效地预测微生物的生产力和细胞生长速度，节省时间和成本，以便优化新的途径，并提供可提供特异性代谢物的变体微生物。

公开(公告)号：KR1020110136441A

公开(公告)日：2011-12-21

申请号：KR1020100056459

申请日：2010-06-15

Applicant: 삼성전자주식회사 ,  고려대학교 산학협력단

Inventor： 구현민 ,  박성민 ,  박재찬 ,  김경헌 ,  최인걸

IPC: C12Q1/26 ,  G01N33/52 ,  G01N30/96

CPC classification number: C12Q1/48 ,  C12N9/1051 ,  G01N2333/91102

Abstract: PURPOSE: A composition for measuring 3,6-anhydro-L-galactose transferase activity is provided to be used in an industry. CONSTITUTION: A composition for measuring 3,6-L-AHG transferase contains NADP+ as a coenzyme, 3,6-anhydro-L-galactose(3,6-L-AHG), and buffer solution. A method for measuring the activity of 3,6-L-AHG transferase comprises: a step of reacting random enzyme extract liquid and a composition for measuring the activity of 3,6-L-AHG tranferase; a step of measuring reduction into NADPH by the reaction. A composition for quantitation of 3,6-L-AHG contains an active fraction containing 3,6-L-AHG tranferase, NADP+ as a coenzyme, and buffer solution.

Abstract translation: 目的：用于测量3,6-脱水-L-半乳糖转移酶活性的组合物用于工业中。 构成：用于测量3,6-L-AHG转移酶的组合物含有作为辅酶的NADP +，3,6-脱水-L-半乳糖（3,6-L-AHG）和缓冲溶液。 测定3,6-L-AHG转移酶的活性的方法包括：使随机酶提取液与用于测定3,6-L-AHG转移酶活性的组合物反应的步骤; 通过反应测量还原成NADPH的步骤。 用于定量3,6-L-AHG的组合物含有含有3,6-L-AHG转移酶，NADP +作为辅酶的活性级分和缓冲溶液。

公开(公告)号：KR1020110106145A

公开(公告)日：2011-09-28

申请号：KR1020100025395

申请日：2010-03-22

Applicant: 삼성전자주식회사 ,  서울대학교산학협력단

Inventor： 구현민 ,  박성민 ,  서진호 ,  박재찬 ,  박용철

IPC: C12P7/06 ,  C12R1/865 ,  C12N15/64 ,  C12N15/66 ,  C12R1/22 ,  C12R1/72

CPC classification number: Y02E50/17 ,  C12P7/06 ,  C12N15/64 ,  C12N15/66 ,  C12R1/22 ,  C12R1/72 ,  C12R1/865 ,  Y10S435/852 ,  Y10S435/921 ,  Y10S435/942

Abstract: 알코올 발효저해물에 대한 저항성을 증대시키는 단리된 폴리뉴클레오티드, 이를 포함하는 벡터, 알코올 생산성 균주 및 이를 이용한 알코올의 생산방법에 관한 기술로서, 특정한 단리된 폴리뉴클레오티드가 호스트셀 내에서 과발현됨으로써 호스트셀의 알코올 발효저해물에 대한 저항성이 증대되므로 알코올 발효시 생산성이 증대될 수 있다.

公开(公告)号：KR1020130094115A

公开(公告)日：2013-08-23

申请号：KR1020120015526

申请日：2012-02-15

Applicant: 삼성전자주식회사

Inventor： 이영민 ,  구현민 ,  김재영 ,  김진우 ,  박재찬 ,  박준성 ,  조화영 ,  이평천

IPC: C12N1/21 ,  C12P7/16 ,  C12N15/52 ,  C12N15/63

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/13 ,  C12N9/88 ,  C12Y101/01001 ,  C12Y101/01016 ,  C12Y102/01016 ,  C12Y102/04002 ,  C12Y203/01019 ,  C12Y207/02007 ,  C12Y208/03005 ,  C12Y401/01071

Abstract: PURPOSE: A deformed microorganism for production of 1,4-butanediol is provided to produce 1,4-BDO by a biological producing process. CONSTITUTION: A deformed microorganism for production of 1.4-butanediol comprises: an activation converting alpha-ketoglutarate or succinate into 4-hydroxybutyril-CoA ("4-HB-CoA"); and an activation converting the 4-HB-CoA into 1,4-butanediols ("1,4-BDO"). The activation converting the 4-HB-CoA into the 1,4-BDO is coded by more than one kind of genes selected from a group comprising adh1, yiaY, adh4, adhB, mdh, eutG, fucO, dhaT, aldA, eutE, adhE1, adhE2 and adh2.

Abstract translation: 目的：提供用于生产1,4-丁二醇的变形微生物，以通过生物制备方法生产1,4-BDO。 构成：用于生产1,4-丁二醇的变形的微生物包括：将α-酮戊二酸或琥珀酸转化成4-羟基丁酰辅酶A（“4-HB-CoA”）的活化; 和将4-HB-CoA转化为1,4-丁二醇（“1,4-BDO”）的活化。 将4-HB-CoA转化为1,4-BDO的激活由选自包含adh1，yyY，adh4，adhB，mdh，eutG，fucO，dhaT，aldA，eutE， adhE1，adhE2和adh2。

公开(公告)号：KR1020130000688A

公开(公告)日：2013-01-03

申请号：KR1020110061366

申请日：2011-06-23

Applicant: 삼성전자주식회사

Inventor： 조화영 ,  유병조 ,  박재찬 ,  박성민 ,  구현민 ,  이주영

IPC: C12Q1/04 ,  C12N1/20 ,  C12N1/14 ,  C12P7/56

CPC classification number: G01N21/80 ,  C12Q1/025

Abstract: PURPOSE: A high throughput screening system and a method of lactic acid-producing strains using two or more pH indicators are provided to quickly and accurately screen strains. CONSTITUTION: A high throughput screening system of lactic acid-producing strains contains the strain, a medium, and two or more pH indicators. The strains are a wild type strain, a mutant strain, and a recombinant strain. The pH indicators contain bromocresol green and methyl red in a ratio of 2:1-10:1. A method for screening the lactic acid-producing strains comprises: a step of adding the pH indicators to a medium; a step of culturing the strains; and a step of observing color change of the pH indicators, and measuring lactic acid production quantity.

Abstract translation: 目的：提供高通量筛选系统和使用两种或多种pH指示剂的乳酸生产菌株的方法，以快速准确筛选菌株。 构成：乳酸生产菌株的高通量筛选系统含有菌株，培养基和两种或多种pH指示剂。 菌株是野生型菌株，突变菌株和重组菌株。 pH指标含有溴甲酚绿和甲基红，比例为2：1-10：1。 筛选乳酸生产菌株的方法包括：将pH指示剂加入到培养基中的步骤; 培养菌株的一个步骤; 以及观察pH指示剂的变色，测定乳酸生成量的工序。

公开(公告)号：KR101860442B1

公开(公告)日：2018-05-24

申请号：KR1020110062312

申请日：2011-06-27

Applicant: 삼성전자주식회사

Inventor： 김재영 ,  박성민 ,  박영경 ,  유병조 ,  박재찬 ,  조화영 ,  구현민

IPC: C12N15/53 ,  C12N15/63 ,  C12P7/52

CPC classification number: C12P7/42 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y101/01001 ,  C12Y101/01027 ,  C12Y203/01008

Abstract: Malonic Semialdehyde 환원경로를이용하여 3-하이드록시프로피온산(3-HP)을생산하는데 있어서, 락테이트탈수소화효소(lactate dehydrogenase), 포스포트란스아세틸라아제(phosphotransacetylase) 및알코올탈수소화효소(alcohol dehydrogenase)의발현을저해함으로써중간대사산물의유출(metabolite leak)을막아 Malonyl-CoA 풀(pool)을증가시켜 3-HP 생성효율을현저히높일수 있는방법에관한것이다.

公开(公告)号：KR101744341B1

公开(公告)日：2017-06-09

申请号：KR1020100056459

申请日：2010-06-15

Applicant: 삼성전자주식회사 ,  고려대학교 산학협력단

Inventor： 구현민 ,  박성민 ,  박재찬 ,  김경헌 ,  최인걸

IPC: C12Q1/26 ,  G01N33/52 ,  G01N30/96

CPC classification number: C12Q1/48 ,  C12N9/1051 ,  G01N2333/91102

Abstract: NADP에서 NADPH로의환원을통해 L-AHG 전환효소의활성을측정하는, 3,6-안하이드로-L-갈락토오스전환효소의활성측정용조성물및 이를이용한 3,6-안하이드로-L-갈락토오스전환효소의활성측정방법을제공한다. 해조류바이오매스등 3,6-안하이드로-L-갈락토오스를포함하는원료의이용에유용하게사용될수 있는바 산업적으로유용하다.

公开(公告)号：KR101690289B1

公开(公告)日：2016-12-28

申请号：KR1020100025395

申请日：2010-03-22

Applicant: 삼성전자주식회사 ,  서울대학교산학협력단

Inventor： 구현민 ,  박성민 ,  서진호 ,  박재찬 ,  박용철

IPC: C12P7/06 ,  C12R1/865 ,  C12N15/64 ,  C12N15/66 ,  C12R1/22 ,  C12R1/72

CPC classification number: Y02E50/17

Abstract: 알코올발효저해물에대한저항성을증대시키는단리된폴리뉴클레오티드, 이를포함하는벡터, 알코올생산성균주및 이를이용한알코올의생산방법에관한기술로서, 특정한단리된폴리뉴클레오티드가호스트셀내에서과발현됨으로써호스트셀의알코올발효저해물에대한저항성이증대되므로알코올발효시생산성이증대될수 있다.