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1.发明公开알데히드 데히드로게나제 변이체, 상기 변이체를 코딩하는 폴리뉴클레오티드, 상기 폴리뉴클레오티드를 포함하는 벡터 및 미생물, 및 이를 이용한 1,4-부탄디올의 생산방법 审中-实审代谢脱氢酶突变体，编码突变体的多核苷酸，载体和具有多核苷酸的微生物，以及使用其制备1,4-丁二醇的方法
公开(公告)号：KR1020150115289A
公开(公告)日：2015-10-14
申请号：KR1020140040064
申请日：2014-04-03
Applicant: 삼성전자주식회사
CPC classification number: C12N9/0008 , C12N9/1025 , C12P7/18 , C12Y102/01003 , C12Y102/0101 , C12Y102/04001 , C12Y108/01004 , C12Y203/03001
Abstract: 알데히드데히드로게나제변이체, 상기변이체를코딩하는폴리뉴클레오티드, 상기폴리뉴클레오티드를포함하는벡터및 미생물, 및이를이용한 1,4-부탄디올의생산방법에관한것이다.
Abstract translation: 本发明涉及醛脱氢酶突变体，编码突变体的多核苷酸，载体和包含该多核苷酸的微生物，以及使用其制备1,4-丁二醇的方法。 醛脱氢酶突变体具有催化从4-羟基丁酰辅酶A向4-羟基丁醛转化的活性。
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公开(公告)号：KR1020130094115A
公开(公告)日：2013-08-23
申请号：KR1020120015526
申请日：2012-02-15
Applicant: 삼성전자주식회사
CPC classification number: C12P7/18 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1217 , C12N9/13 , C12N9/88 , C12Y101/01001 , C12Y101/01016 , C12Y102/01016 , C12Y102/04002 , C12Y203/01019 , C12Y207/02007 , C12Y208/03005 , C12Y401/01071
Abstract: PURPOSE: A deformed microorganism for production of 1,4-butanediol is provided to produce 1,4-BDO by a biological producing process. CONSTITUTION: A deformed microorganism for production of 1.4-butanediol comprises: an activation converting alpha-ketoglutarate or succinate into 4-hydroxybutyril-CoA ("4-HB-CoA"); and an activation converting the 4-HB-CoA into 1,4-butanediols ("1,4-BDO"). The activation converting the 4-HB-CoA into the 1,4-BDO is coded by more than one kind of genes selected from a group comprising adh1, yiaY, adh4, adhB, mdh, eutG, fucO, dhaT, aldA, eutE, adhE1, adhE2 and adh2.
Abstract translation: 目的：提供用于生产1,4-丁二醇的变形微生物，以通过生物制备方法生产1,4-BDO。 构成：用于生产1,4-丁二醇的变形的微生物包括：将α-酮戊二酸或琥珀酸转化成4-羟基丁酰辅酶A（“4-HB-CoA”）的活化; 和将4-HB-CoA转化为1,4-丁二醇（“1,4-BDO”）的活化。 将4-HB-CoA转化为1,4-BDO的激活由选自包含adh1，yyY，adh4，adhB，mdh，eutG，fucO，dhaT，aldA，eutE， adhE1，adhE2和adh2。
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公开(公告)号：KR1020130000688A
公开(公告)日：2013-01-03
申请号：KR1020110061366
申请日：2011-06-23
Applicant: 삼성전자주식회사
Abstract: PURPOSE: A high throughput screening system and a method of lactic acid-producing strains using two or more pH indicators are provided to quickly and accurately screen strains. CONSTITUTION: A high throughput screening system of lactic acid-producing strains contains the strain, a medium, and two or more pH indicators. The strains are a wild type strain, a mutant strain, and a recombinant strain. The pH indicators contain bromocresol green and methyl red in a ratio of 2:1-10:1. A method for screening the lactic acid-producing strains comprises: a step of adding the pH indicators to a medium; a step of culturing the strains; and a step of observing color change of the pH indicators, and measuring lactic acid production quantity.
Abstract translation: 目的：提供高通量筛选系统和使用两种或多种pH指示剂的乳酸生产菌株的方法，以快速准确筛选菌株。 构成：乳酸生产菌株的高通量筛选系统含有菌株，培养基和两种或多种pH指示剂。 菌株是野生型菌株，突变菌株和重组菌株。 pH指标含有溴甲酚绿和甲基红，比例为2：1-10：1。 筛选乳酸生产菌株的方法包括：将pH指示剂加入到培养基中的步骤; 培养菌株的一个步骤; 以及观察pH指示剂的变色，测定乳酸生成量的工序。
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公开(公告)号：KR1020120099315A
公开(公告)日：2012-09-10
申请号：KR1020110007877
申请日：2011-01-26
Applicant: 삼성전자주식회사
CPC classification number: C12Y402/0103 , C12N9/0006 , C12N9/0008 , C12N9/88 , C12P7/18 , C12P7/42 , C12Y101/01202 , C12Y102/01
Abstract: PURPOSE: A method for preparing 3-HP(3-hydroxypropionic acid) and 1,3-PDO(1,3-propandiol) using microorganisms is provided to convert a glycerol substrate into 3-HP and 1,3-PDO. CONSTITUTION: A recombinant microorganism for preparing 3-HP and 1,3-PDO contains genes producing the compounds. The genes are dhaB(glycerol dehydratease), alhH(aldehyde dehydrogenase), and dhaT(1,3-propandiol oxidoreductase). dhaB gene is derived from K. pneumoniae or C. butyricum. alhH gene is derived from E.coli. The recombinant microorganism is E.coli. A method for producing 3-HP and 1,3-PDO comprises a step of culturing the recombinant microorganism in a medium containing a carbon substrate. The carbon substrate is glucose, sucrose, cellulose, or glycerol.
Abstract translation: 目的：提供使用微生物制备3-HP（3-羟基丙酸）和1,3-PDO（1,3-丙二醇）的方法，以将甘油底物转化为3-HP和1,3-PDO。 构成：用于制备3-HP和1,3-PDO的重组微生物含有产生该化合物的基因。 基因是dhaB（甘油脱水酶），alhH（醛脱氢酶）和dhaT（1,3-丙二醇氧化还原酶）。 dhaB基因衍生自肺炎克雷伯杆菌或C.丁酸菌。 alhH基因来自大肠杆菌。 重组微生物是大肠杆菌。 制备3-HP和1,3-PDO的方法包括在含有碳底物的培养基中培养重组微生物的步骤。 碳底物是葡萄糖，蔗糖，纤维素或甘油。
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Patent Agency Ranking
公开(公告)号：KR101860442B1
公开(公告)日：2018-05-24
申请号：KR1020110062312
申请日：2011-06-27
Applicant: 삼성전자주식회사
CPC classification number: C12P7/42 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y101/01027 , C12Y203/01008
Abstract: Malonic Semialdehyde 환원경로를이용하여 3-하이드록시프로피온산(3-HP)을생산하는데 있어서, 락테이트탈수소화효소(lactate dehydrogenase), 포스포트란스아세틸라아제(phosphotransacetylase) 및알코올탈수소화효소(alcohol dehydrogenase)의발현을저해함으로써중간대사산물의유출(metabolite leak)을막아 Malonyl-CoA 풀(pool)을증가시켜 3-HP 생성효율을현저히높일수 있는방법에관한것이다.
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公开(公告)号：KR101657100B1
公开(公告)日：2016-09-19
申请号：KR1020090060397
申请日：2009-07-02
Applicant: 삼성전자주식회사
CPC classification number: C10L1/02 , A23V2002/00 , C08B37/0057 , C08H8/00 , C10G2300/1011 , C12M45/06 , C12P7/10 , C12P2201/00 , C13K13/002 , D21C1/04 , D21C1/06 , D21C5/00 , Y02E50/16 , Y02E50/17 , Y02P30/20 , A23V2250/5108 , A23V2250/638
Abstract: 리그노셀룰로오스계바이오매스의분별방법에관한기술로서, 리그노셀룰로오스계바이오매스를제공하고, 리그닌을용해시키는용매를부가하여바이오매스로부터리그닌을추출한후, 여기에헤미셀룰로오스를용해시키는용매를부가하여자일로오스를추출하며, 바이오매스로부터잔류한셀룰로오스를추출할수 있다. 이러한방법에의할경우공정효율이낮은회분식(배취식) 방법이아니라연속식공정으로수행할수 있고, 바이오매스의각 성분을고수율로획득할수 있다.
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公开(公告)号：KR1020100093253A
公开(公告)日：2010-08-25
申请号：KR1020090012359
申请日：2009-02-16
Applicant: 삼성전자주식회사 , 경기대학교 산학협력단
CPC classification number: C12P19/02 , C12M47/06 , C12M47/14 , C12P5/02 , C12P7/04 , C12P7/10 , C12P7/649 , Y02E50/13 , Y02E50/16 , Y02E50/17 , Y02P20/136 , Y02P30/20
Abstract: PURPOSE: A method and apparatus for pretreatment and saccharification of sea algae biomass is provided to prevent reduction of saccharide concentration regardless of time and space. CONSTITUTION: A method for pretreatment/saccharification of sea algae biomass comprises: a dehydrating sea algae bio mass by 10-70% of water content; a step of cutting the biomass in a certain size; and a step of treating the biomass with hydrolysis catalyst and/or hydrolase. An apparatus for pretreatment/saccharification of sea algae biomass comprises: a transfer pipe having a hollow as a transfer path of the sea algae biomass; an input port for inputting the biomass; a screw which provides the transfer driving force of the biomass by shaft rotation; a dehydrating device with an outlet for discharging the biomass; a cutting device which is connected to the one end of outlet side; and a saccharification device having a reactor for saccharification.
Abstract translation: 目的：提供海藻生物质的预处理和糖化的方法和装置，以防止糖浓度的降低，而不管时间和空间如何。 构成：海藻生物质预处理/糖化的方法包括：脱水海藻生物质含量为10-70％; 以一定尺寸切割生物质的步骤; 以及用水解催化剂和/或水解酶处理生物质的步骤。 一种用于海藻生物质的预处理/糖化的设备包括：具有作为海藻生物质的转移路径的中空的输送管; 用于输入生物量的输入端口; 螺杆，其通过轴旋转提供生物质的传递驱动力; 具有用于排出生物质的出口的脱水装置; 连接到出口侧的一端的切割装置; 和具有糖化反应器的糖化装置。
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公开(公告)号：KR1020100047789A
公开(公告)日：2010-05-10
申请号：KR1020090060397
申请日：2009-07-02
Applicant: 삼성전자주식회사
CPC classification number: C10L1/02 , A23V2002/00 , C08B37/0057 , C08H8/00 , C10G2300/1011 , C12M45/06 , C12P7/10 , C12P2201/00 , C13K13/002 , D21C1/04 , D21C1/06 , D21C5/00 , Y02E50/16 , Y02E50/17 , Y02P30/20 , A23V2250/5108 , A23V2250/638
Abstract: PURPOSE: A sorting method of lignocellulose-based biomass and a sorting device thereof are provided to improve processing efficiency through a continuous process, and to prevent excessive decomposition of xylose by successively extracting lignin and xylose. CONSTITUTION: A sorting method of lignocellulose-based biomass includes the following steps: offering lignocellulose-based biomass(S1); extracting lignin from biomass by adding a first solvent dissolving the lignin(S2); extracting xylose by adding a second solvent dissolving hemicelluloses(S3); and extracting the remaining cellulose from the extracted biomass. A sorting device includes a reaction vessel dipping the biomass, a second solvent tank, a first solvent tank, a first reservoir storing the lignin, and a reservoir including a second reservoir.
Abstract translation: 目的：提供基于木质纤维素的生物质的分选方法及其分选装置，以通过连续方法提高加工效率，并通过连续提取木质素和木糖来防止木糖过度分解。 构成：基于木素纤维素的生物质的分选方法包括以下步骤：提供基于木质纤维素的生物质（S1）; 通过添加溶解木质素的第一溶剂（S2）从生物质中提取木质素; 通过加入溶解半纤维素的第二溶剂提取木糖（S3）; 并从提取的生物质中提取剩余的纤维素。 分选装置包括浸渍生物质的反应容器，第二溶剂罐，第一溶剂罐，储存木质素的第一储存器和包括第二储存器的储存器。
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公开(公告)号：KR101569013B1
公开(公告)日：2015-11-16
申请号：KR1020090012359
申请日：2009-02-16
Applicant: 삼성전자주식회사 , 경기대학교 산학협력단
CPC classification number: C12P19/02 , C12M47/06 , C12M47/14 , C12P5/02 , C12P7/04 , C12P7/10 , C12P7/649 , Y02E50/13 , Y02E50/16 , Y02E50/17 , Y02P20/136 , Y02P30/20
Abstract: 해조류바이오매스의전처리/당화방법에관한기술로서, 해조류바이오매스를완전건조및 분말화하는과정을거치지않고, 자연에서채취/수득한해조류를탈수및 절단하는과정을통해습식공정으로전처리하여당화시키는해조류바이오매스의전처리/당화방법을제공한다. 따라서, 해조류바이오매스의건조/분말화과정에따른시간적·공간적제약을받지않으며고비용, 저효율의문제를해소할수 있다. 또한, 당화를위한산처리시고체산의이용이가능함으로써액체산을사용하는경우발생하는당농도저하를방지할수 있다.
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