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    맥강 단백질 이용한 미네랄 결합 펩타이드의 제조방법
    12.
    发明公开
    맥강 단백질 이용한 미네랄 결합 펩타이드의 제조방법 有权
    巴氏蛋白质矿物结合肽的制备方法

    公开(公告)号：KR1020130012688A

    公开(公告)日：2013-02-05

    申请号：KR1020110073987

    申请日：2011-07-26

    Applicant: 충남대학교산학협력단

    Inventor： 송경빈 ,  이지혜 ,  최동원

    IPC: C12P21/06 ,  C07K1/34 ,  C07K14/415

    CPC classification number: C12P21/06 ,  B01D15/26 ,  B01D15/36 ,  C07K1/16 ,  C07K1/18 ,  C07K1/34

    Abstract: PURPOSE: A barley bran-derived mineral binding peptide and a method for preparing the same are provided to enhance the absorption of mineral enriched food and nutritional supplement products into a body. CONSTITUTION: A method for preparing a mineral binding peptide derived from barley bran comprises: a step of treating barley bran with alkali or acid, and extracting proteins; a step of treating the proteins with hydrolase and preparing a hydrolysate; a step of performing ultrafiltration of the hydrolysate; and a step of reacting the hydrolysate and mineral, and isolating a peptide with a mineral binding activity. The acid is normal hexane. The barley bran is dissolved with normal hexane in ratio of 1:3(w/w). [Reference numerals] (AA) Flow chart of a method for extracting barley bran protein; (BB) Barley bran:normal hexane(1:3, w/w); (CC) Centrifugation after stirring for 3 hours(7,000xg, 30minutes); (DD) Drying after repeating twice; (EE) Barley bran coat:distilled water(1:5, w/w); (FF) Ultrasonic wave pulverizer(10 minutes); (GG) PH adjustment(3N sodium hydroxide, pH 9.5); (HH) Centrifugation after stirring for 2 hours(10,000xg, 30 minutes); (II) PH adjustment using supernatant(2N hydrochloric acid, pH 4.5); (JJ) Centrifugation after stirring for 1 hour(5,000xg, 20 minutes); (KK) Washing precipitate using distilled water(5,000xg, 10 minutes), twice; (LL) Freeze-drying

    Abstract translation: 目的：提供大麦麸衍生的矿物结合肽及其制备方法，以增强富含矿物质的食物和营养补充品吸收到体内。 构成：用于制备源自大麦麸的矿物结合肽的方法包括：用碱或酸处理大麦麸的步骤，提取蛋白质; 用水解酶处理蛋白质并制备水解产物的步骤; 进行水解产物的超滤的步骤; 以及使水解产物和矿物质反应并分离具有矿物结合活性的肽的步骤。 酸是正己烷。 用1:5（w / w）比例的正己烷溶解大麦麸皮。 （附图标记）（AA）提取大麦麸蛋白的方法的流程图; （BB）大麦麸：正己烷（1:3，w / w）; （CC）搅拌3小时后离心（7,000xg，30分钟）; （DD）重复两次后干燥; （EE）大麦麸皮：蒸馏水（1:5，w / w）; （FF）超声波粉碎机（10分钟）; （GG）PH调节（3N氢氧化钠，pH9.5）; （HH）搅拌2小时后离心（10,000xg，30分钟）; （II）使用上清液（2N盐酸，pH4.5）进行PH调节; （JJ）搅拌1小时后离心（5,000×g，20分钟）; （KK）使用蒸馏水（5,000xg，10分钟）洗涤沉淀2次; （LL）冷冻干燥

    고분자 전해질 다층박막과 마이크로 컨택트 프린팅을이용한 단백질 고정화방법
    17.
    发明公开
    고분자 전해질 다층박막과 마이크로 컨택트 프린팅을이용한 단백질 고정화방법 有权
    使用大分子电解质薄膜薄膜和微晶印刷的蛋白质固定方法

    公开(公告)号：KR1020090128286A

    公开(公告)日：2009-12-15

    申请号：KR1020080054372

    申请日：2008-06-10

    Applicant: 충남대학교산학협력단

    Inventor： 이창수 ,  이지혜

    IPC: G01N33/68 ,  G01N33/53 ,  G01N33/48

    Abstract: PURPOSE: A selective protein fixation is provided to enhance probe efficiency in a protein chip and accurately diagnose. CONSTITUTION: A protein is selectively fixed on a surface treated with layer-by-layer deposition(LBL) using static attraction using polyeletrolyte. The polyelectrolyte is polyarylamine hydro chloride(PAH) or polysodium 4-styrene sulfonate(PSS). The PAH is 20Mm(Ph 9.0) and PSS is 60 Mm(pH 7.0). A method for fixing a protein comprises: a step of producing a substrate of multi layer thin film using polyelectrolyte; a step of making a micro-stamp with micro pattern; and a step of fixing protein through micro-contacting printing method using micro stamp.

    Abstract translation: 目的：提供选择性蛋白质固定以提高蛋白质芯片中的探针效率并准确诊断。 构成：蛋白质选择性地固定在用逐层沉积（LBL）处理的表面上，使用聚乙烯混合溶液进行静态吸引。 聚电解质是聚芳基胺氯化氢（PAH）或4-苯乙烯磺酸钠（PSS）。 PAH为20Mm（Ph 9.0），PSS为60Mm（pH7.0）。 固定蛋白质的方法包括：使用聚电解质制造多层薄膜的基板的步骤; 制作微图案微印的步骤; 以及使用微印刷通过微接触印刷法固定蛋白质的步骤。

    세포의 고정화를 위한 기판의 표면 처리 방법
    18.
    发明公开
    세포의 고정화를 위한 기판의 표면 처리 방법 有权
    用于固定细胞的表面处理方法

    公开(公告)号：KR1020080075379A

    公开(公告)日：2008-08-18

    申请号：KR1020070014472

    申请日：2007-02-12

    Applicant: 충남대학교산학협력단

    Inventor： 이창수 ,  심현우 ,  이지혜 ,  강경석

    IPC: C12N11/00 ,  G01N33/48 ,  C12M1/00 ,  H01L21/306

    Abstract: A surface treatment method for the fixation of cells is provided to improve economical efficiency, rapidness and yield of cell fixation by simplifying the cell fixation process, and reducing non-specific adsorption and binding of cells. A surface treatment method for the fixation of cells by using layer-by-layer deposition(LBL) comprises the steps of: (i) treating a substrate with oxygen plasma cleaner to have anions on the substrate surface; (ii) treating the substrate surface with a first polymer electrolyte having a plurality of cations such as polyallylamine hydrochloride(PAH) represented by the formula(1) or poly(4-ammonium styrene sulfonic acid)(PSS) represented by the formula(2) and washing the treated substrate; (iii) treating the substrate surface with a second polymer electrolyte having a plurality of anions and washing the treated substrate; and (iv) repeating the steps (ii) and (iii), further comprises a step of constructing a three-dimensional micro structure by using polyethylene glycol dimethacrylate(PEG-DMA) represented by the formula(3) and D1173 photo initiator. Further, an average molecular of polymer for producing the three-dimensional micro structure is 300 to 30000.

    Abstract translation: 提供细胞固定的表面处理方法，通过简化细胞固定过程，降低细胞的非特异性吸附和结合，提高细胞固定的经济效率，快速性和产率。 用于通过逐层沉积（LBL）固定细胞的表面处理方法包括以下步骤：（i）用氧等离子体清洁剂处理基底以在基底表面上具有阴离子; （ii）用具有多个阳离子的第一聚合物电解质处理底物表面，所述阳离子例如由式（1）表示的聚烯丙胺盐酸盐（PAH）或由式（2）表示的聚（4-氨基苯乙烯磺酸）（PSS） ）并洗涤经处理的基材; （iii）用具有多个阴离子的第二聚合物电解质处理基材表面并洗涤经处理的基材; 和（iv）重复步骤（ii）和（iii），还包括通过使用由式（3）表示的聚乙二醇二甲基丙烯酸酯（PEG-DMA）和D1173光引发剂来构建三维微结构的步骤。 此外，用于制造三维微结构的聚合物的平均分子量为300〜30000。

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