Abstract:
The present invention provides a model and technology which selects a high capacity impotent cow having excellent heredity ability and collects eggs in vivo so as to enable mass propagation of excellent fertilized eggs by biotechnology technique, embryo transfer technology, etc., thereby acquiring, preserving, and maintaining outstanding genetic resources of hanwoo (Korean native cattle) in farm, and moreover, a high-quality meat production system is prepared so as to increase recessed hanwoo breeding farms and ensure competitiveness of hanwoo which is differentiated to imported meat.
Abstract:
본발명은한국산재래닭의정강이색깔에관여하는 DNA 표지인자개발에관한것으로서, 한국산재래닭의정강이색깔에대한유전능력을조기에예측하고식별할수 있는 DNA 표지인자를개발하여한국산재래닭의경제형질개선또는육종개량프로그램에이용하는데 있다. 본발명의 DNA 표지인자를한국산재래닭의정강이색을확인할수 있는한국산재래닭의조기선발도구로활용하는경우, 소비자가한국산재래닭에요구하는정강이색, 구체적으로밝은노란색에가까운노란색의정강이색을갖는수입산브로일러에비하여어두운색즉, 명도가낮은정강이색을가진한국산재래닭으로의육종개량은보다효율적이고경제적으로추진할수 있으며, 이를통하여재래닭사육농가의소득증대및 경영개선에이바지할수 있다.
Abstract:
PURPOSE: A genetic marker for identifying fatty acid composition of Hanwoo(Korean beef) is provided to easily select Hanwoo with high quality. CONSTITUTION: A method for selecting Hanwoo with high unsaturated fatty acid content comprises: a step of isolating genome DNA sample from a Hanwoo; a step of amplifying PCR of a genetic marker of sequence number 1 containing SNP at 21th base using a primer; a step of cleaving a PCR product with restriction enzyme; a step of performing electrophoresis to identify fragment number and size; and a step of detecting genotype of 21th base. The primer is a primer of sequence numbers 3 and 4. The restriction enzyme is Rsal.
Abstract:
본발명은한국산재래닭의정강이색깔에관여하는 DNA 표지인자개발에관한것으로서, 한국산재래닭의정강이색깔에대한유전능력을조기에예측하고식별할수 있는 DNA 표지인자를개발하여한국산재래닭의경제형질개선또는육종개량프로그램에이용하는데 있다. 본발명의 DNA 표지인자를한국산재래닭의정강이색을확인할수 있는한국산재래닭의조기선발도구로활용하는경우, 소비자가한국산재래닭에요구하는정강이색, 구체적으로밝은노란색에가까운노란색의정강이색을갖는수입산브로일러에비하여어두운색즉, 명도가낮은정강이색을가진한국산재래닭으로의육종개량은보다효율적이고경제적으로추진할수 있으며, 이를통하여재래닭사육농가의소득증대및 경영개선에이바지할수 있다.
Abstract:
PURPOSE: A method for analyzing SNP of SCD(Stearoyl-CoA Desaturase) gene of a chicken is provided to produce and select high quality chickens, to improve farm income, and to increase sales. CONSTITUTION: A method for selecting a chicken with higher unsaturated fatty acid content than a chicken with heterozygous genotype at 2nd exon of chicken SCD gene comprises: a step of collecting a DNA sample from the chicken; a step of amplifying the DNA sample using the primer set of sequence numbers 1 and 2; a step of treating the amplified product with Acil; and a step of performing electrophoresis and identifying whether SNP of the 2nd exon of SCD is a homozygous genotype.
Abstract:
본 발명은 돼지 유전자에 삽입되어 있는 PERV의 연접서열 및 이를 이용한 PERV 검출용 프라이머 쌍에 관한 것으로서, 연접서열인 서열번호 1 내지 14 중 어느 하나를 포함하는 염기서열과, ① 상기 연접서열을 포함하는 염기서열에 상보적인 서열을 갖는 올리고 뉴클레오티드와, ② PERV 유전자 염기서열에 상보적인 서열을 갖는 올리고 뉴클레오티드로 이루어진 돼지 게놈 내 융합된 PERV 검출을 위한 프라이머 쌍에 관한 것이다. 본 발명에 의하면 돼지의 조직 또는 장기로부터 이종이식 시 문제의 소지가 있는 PERV의 존재를 사전에 검출할 수 있으며, 더 나아가 해당 유전자를 녹아웃(knock-out)시키는 데 이용할 수 있다. 돼지 내인성 레트로바이러스(PERV), 장기이식, 검출, 프라이머, 유전좌위, knock-out
Abstract:
A sequence of PERV (porcine endogenous retrovirus) and PERV primer pair using the same are provided to previously detect the presence of PERV and use for knock-out of a gene. A 3'-, or 5'-sequence to a porcine endogenous retrovirus (PERV) which is a sequence selected from the sequence numbers 1 to 14. A primer pair for detecting PERV comprises an oligonucleotide which has complementary sequence to the sequence numbers 1 to 14 and an oligonucleotide which has complementary sequence to the PERV gene sequence. The primer pair is used for detecting PERV through PCR (polymerase chain reaction) from porcine tissue or organ.