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公开(公告)号：KR101472323B1
公开(公告)日：2014-12-15
申请号：KR1020120041710
申请日：2012-04-20
Applicant: 경상대학교산학협력단 , 충남대학교산학협력단
Abstract: 본발명은건강한교잡종돼지개체를선별하기위해, 서열번호 1의핵산서열로부터선택되며, 상기서열번호 1의 1,817번째또는 15,384번째염기인 SNP를포함하는 10개이상의연속적인핵산으로구성되는교잡종돼지의지방산조성예측용폴리뉴클레오티드또는그에상보적인폴리뉴클레오티드를제공한다.
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Patent Agency Ranking
公开(公告)号：KR101160794B1
公开(公告)日：2012-06-27
申请号：KR1020110138839
申请日：2011-12-21
Applicant: 충청남도 , 충남대학교산학협력단
CPC classification number: C12Q1/6827 , C12Q1/6888 , C12Q2531/113 , C12Q2600/124 , C12Q2600/156
Abstract: PURPOSE: A genetic marker for identifying fatty acid composition of Hanwoo(Korean beef) is provided to easily select Hanwoo with high quality. CONSTITUTION: A method for selecting Hanwoo with high unsaturated fatty acid content comprises: a step of isolating genome DNA sample from a Hanwoo; a step of amplifying PCR of a genetic marker of sequence number 1 containing SNP at 21th base using a primer; a step of cleaving a PCR product with restriction enzyme; a step of performing electrophoresis to identify fragment number and size; and a step of detecting genotype of 21th base. The primer is a primer of sequence numbers 3 and 4. The restriction enzyme is Rsal.
Abstract translation: 目的：用于确定Hanwoo（韩国牛肉）脂肪酸组成的遗传标记，可轻松选择高质量的Hanwoo。 构成：选择具有高不饱和脂肪酸含量的Hanwoo的方法包括：从Hanwoo分离基因组DNA样品的步骤; 使用引物扩增第21位含有SNP的序列号1的遗传标记物的PCR的步骤; 用限制酶切割PCR产物的步骤; 进行电泳以识别片段数目和大小的步骤; 检测第21位基因型的步骤。 引物是序列号3和4的引物。限制酶是Rsal。
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3.发明授权
公开(公告)号：KR101273333B1
公开(公告)日：2013-06-11
申请号：KR1020120075835
申请日：2012-07-12
Applicant: 충남대학교산학협력단
CPC classification number: C12Q1/683 , C12Q2600/124 , C12Q2600/156
Abstract: PURPOSE: A method for analyzing SNP of SCD(Stearoyl-CoA Desaturase) gene of a chicken is provided to produce and select high quality chickens, to improve farm income, and to increase sales. CONSTITUTION: A method for selecting a chicken with higher unsaturated fatty acid content than a chicken with heterozygous genotype at 2nd exon of chicken SCD gene comprises: a step of collecting a DNA sample from the chicken; a step of amplifying the DNA sample using the primer set of sequence numbers 1 and 2; a step of treating the amplified product with Acil; and a step of performing electrophoresis and identifying whether SNP of the 2nd exon of SCD is a homozygous genotype.
Abstract translation: 目的：提供一种分析鸡的SCD（硬脂酰CoA去饱和酶）基因SNP的方法，以生产和选择优质鸡，提高农民收入，增加销售。 构成：选择鸡SCD基因第二外显子上具有杂合子基因型的鸡的不饱和脂肪酸含量高的鸡的方法包括：从鸡收集DNA样品的步骤; 使用序列号1和2的引物组扩增DNA样品的步骤; 用Acil处理扩增产物的步骤; 以及进行电泳并鉴定SCD的第二外显子的SNP是否是纯合基因型的步骤。
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公开(公告)号：KR1020130118650A
公开(公告)日：2013-10-30
申请号：KR1020120041710
申请日：2012-04-20
Applicant: 경상대학교산학협력단 , 충남대학교산학협력단
CPC classification number: C12Q1/6888 , C12Q2600/124 , C12Q2600/156
Abstract: PURPOSE: A SNP marker for predicting fatty acid composition of a crossbreed pig is provided to predict fatty acid composition, especially for a composition of unsaturated fatty acid and saturated fatty acid, thereby utilizing the SNP marker for selection of healthy pig entity and high value pork production. CONSTITUTION: A polynucleotide or corresponding polynucleotide for predicting fatty acid composition of a crossbreed pig is selected from polynucleotide having nucleic acid sequence of sequence number 1. The polynucleotide or corresponding polynucleotide comprises 0 or more of successive nucleic acids which include 817th of the sequence number 1 or 14384th SNP related base. The crossbreed pig is a crossbreed between Korea native kind and Landrace. The crossbreed pig is F_2. A polynucleotide which specifically mix with the polynucleotide or the corresponding polynucleotide is provided.
Abstract translation: 目的：提供用于预测杂交猪的脂肪酸组成的SNP标记，以预测脂肪酸组成，特别是对于不饱和脂肪酸和饱和脂肪酸的组成，从而利用SNP标记物选择健康猪实体和高价值猪肉 生产。 构成：用于预测杂交猪的脂肪酸组成的多核苷酸或相应的多核苷酸选自具有序列号1的核酸序列的多核苷酸。多核苷酸或相应的多核苷酸包含0或更多个连续核酸，其包含序列号1的第817位 或第14384位SNP相关基地。 杂交猪是韩国本土和兰德拉斯之间的杂交种。 杂交猪是F_2。 提供了与多核苷酸或相应多核苷酸特异性混合的多核苷酸。
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公开(公告)号：KR100980809B1
公开(公告)日：2010-09-10
申请号：KR1020080022720
申请日：2008-03-12
Applicant: 충남대학교산학협력단
Abstract: 본 발명은 돼지 유전자에 삽입되어 있는 PERV의 연접서열 및 이를 이용한 PERV 검출용 프라이머 쌍에 관한 것으로서, 연접서열인 서열번호 1 내지 14 중 어느 하나를 포함하는 염기서열과, ① 상기 연접서열을 포함하는 염기서열에 상보적인 서열을 갖는 올리고 뉴클레오티드와, ② PERV 유전자 염기서열에 상보적인 서열을 갖는 올리고 뉴클레오티드로 이루어진 돼지 게놈 내 융합된 PERV 검출을 위한 프라이머 쌍에 관한 것이다.
본 발명에 의하면 돼지의 조직 또는 장기로부터 이종이식 시 문제의 소지가 있는 PERV의 존재를 사전에 검출할 수 있으며, 더 나아가 해당 유전자를 녹아웃(knock-out)시키는 데 이용할 수 있다.
돼지 내인성 레트로바이러스(PERV), 장기이식, 검출, 프라이머, 유전좌위, knock-out-
公开(公告)号：KR1020090097526A
公开(公告)日：2009-09-16
申请号：KR1020080022720
申请日：2008-03-12
Applicant: 충남대학교산학협력단
CPC classification number: C12Q1/686
Abstract: A sequence of PERV (porcine endogenous retrovirus) and PERV primer pair using the same are provided to previously detect the presence of PERV and use for knock-out of a gene. A 3'-, or 5'-sequence to a porcine endogenous retrovirus (PERV) which is a sequence selected from the sequence numbers 1 to 14. A primer pair for detecting PERV comprises an oligonucleotide which has complementary sequence to the sequence numbers 1 to 14 and an oligonucleotide which has complementary sequence to the PERV gene sequence. The primer pair is used for detecting PERV through PCR (polymerase chain reaction) from porcine tissue or organ.
Abstract translation: 提供了使用其的PERV（猪内源性逆转录病毒）和PERV引物对的序列，以便先前检测PERV的存在并用于敲除基因。 与选自序列号1〜14的序列的猪内源性逆转录病毒（PERV）的3'-或5'序列。检测PERV的引物对包含与序列号1〜 14和与PERV基因序列具有互补序列的寡核苷酸。 引物对用于通过PCR（聚合酶链反应）从猪组织或器官检测PERV。
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