公开(公告)号：KR1020090109809A

公开(公告)日：2009-10-21

申请号：KR1020080035223

申请日：2008-04-16

Applicant: 포항공과대학교 산학협력단

Inventor： 이돈욱 ,  류성호 ,  하상훈 ,  정현태 ,  마이낙베너지 ,  셀바팔람나라야난 ,  김기문 ,  박경민

IPC: C12N15/10

CPC classification number: C07K1/22

Abstract: PURPOSE: A method for isolating or purifying cellular components through non-covalent bond of cucurbituril derivative and guest compound is provided to isolate various compounds. CONSTITUTION: A method for isolating or purifying cellular components through non-covalent bond of cucurbituril derivative and guest compound comprises: a step of contacting reactive compound with cells; a step of performing lysis the cells; a step of adding solid phase which is connected with a host compound in the solution in which cells are lyzed; a step of isolating and purifying the guest compound and host compound; and a step of isolating cell component.

Abstract translation: 目的：提供通过葫芦素衍生物和客体化合物的非共价键分离或纯化细胞成分的方法以分离各种化合物。 构成：通过葫芦巴衍生物和客体化合物的非共价键分离或纯化细胞成分的方法包括：使反应性化合物与细胞接触的步骤; 细胞裂解步骤; 在将细胞溶解的溶液中添加与主体化合物连接的固相的工序; 分离和纯化客体化合物和主体化合物的步骤; 以及隔离细胞成分的步骤。