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公开(公告)号：KR1020000056471A
公开(公告)日：2000-09-15
申请号：KR1019990005821
申请日：1999-02-22
Applicant: 한국과학기술연구원
IPC: C12N1/16
Abstract: PURPOSE: New strain of Candidanorvegica, CRM-9802 (KCTC 8900P) containing high concentration of ribonucleic acid (RNA) is provided which is used to manufacture a nucleotide spice from RNA of Candida cell extract. CONSTITUTION: Soil sample is mixed with sterile saline and vortexed. The supernatant is plated on YM agar and incubated at 30°C for 24-48 hours. The isolated colony is cultured and RNA content of each colony is measured. The colony showing high content of RNA is selected and its morphological, physiological and cultural characteristics are identified. The isolated yeast forms creamy egg shape colony on YM plate and does not make spores. The range of temperature for growth is from 15 to 35°C. Under anaerobic condition it metabolizes D-glucose and D-cellobiose as carbon sources. On the other hand, D-xylose, L-rhamnose, cellobios, salicin, arbutin, glycerol, D-glucitol, citrate and ethanol can be metabolized at aerobic conditions. Nitrate, nitrite, L-lysine and creatine can be used as a nitrogen source at aerobic conditions. The yeast is cultured in YM medium and harvested at 10 OD and RNA content of cell is measured by orcinol method. The RNA content of Candida norvegica is 12-13% of cell mass.
Abstract translation: 目的：提供含有高浓度核糖核酸（RNA）的新一代Candidanorvegica，CRM-9802（KCTC 8900P），用于从念珠菌细胞提取物的RNA制备核苷酸调味剂。 构成：将土壤样品与无菌盐水混合并涡旋。 将上清液接种在YM琼脂上，并在30℃下孵育24-48小时。 培养分离的菌落，测定每个菌落的RNA含量。 选择显示高含量RNA的菌落，确定其形态，生理和文化特征。 分离的酵母在YM板上形成奶油蛋形菌落，不产生孢子。 生长温度范围为15〜35℃。 在厌氧条件下，将D-葡萄糖和D-纤维二糖代谢为碳源。 另一方面，D-木糖，L-鼠李糖，纤维二糖，水杨蛋白，熊果苷，甘油，D-葡萄糖醇，柠檬酸盐和乙醇可以在需氧条件下代谢。 硝酸盐，亚硝酸盐，L-赖氨酸和肌酸可用作有氧条件下的氮源。 将酵母在YM培养基中培养并在10℃下收获，并通过α-淀粉酶法测定细胞的RNA含量。 假丝酵母的RNA含量为细胞质量的12-13％。
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12.发明公开신규 락토바실러스 속 (LACTOBACILLUS SP.)MT-1077 및 그로부터 생산되는 신규 박테리오신 失效新颖的LACTOBACILLUS SP。 MT-1077（KCTC 8903P）和新生成的细菌
公开(公告)号：KR1020000047065A
公开(公告)日：2000-07-25
申请号：KR1019980063818
申请日：1998-12-31
Applicant: 한국과학기술연구원
Abstract: PURPOSE: A novel lactobacillus sp. MT-1077 (KCTC 8903P) and a novel bacteriocin produced therefrom are provided. The bacteriocin has stability against broad range of pH, heat stability and antifungal activity and used as food preservation agent, biological fermentation regulator, and antifungal agent. CONSTITUTION: A lactobacillus MT-1077 is isolated from Kimchi. The method for purifying bacteriocin from lactobacillus MT-1077 comprises the steps of: incubating lactobacillus MT-1077 in MRS broth; precipitating the lactobacillus MT-1077 fermented culture broth by addition of ammonium hydrogen sulfate; passing the protein precipitates through the column packed with carboxymethyl sepharose CL-6B and eluting the adsorbed protein with 0.1M sodium chloride; passing the elutes through the column packed with phenyl sepharose CL-4B and eluting the adsorbed protein with 10% ammonium hydrogen sulfate solution; and purifying the elutes by HPLC
Abstract translation: 目的：一种新型乳酸杆菌 提供MT-1077（KCTC 8903P）和由其制备的新型细菌素。 细菌素具有广泛的pH，热稳定性和抗真菌活性的稳定性，并且用作食品保存剂，生物发酵调节剂和抗真菌剂。 构成：从泡菜中分离出一种乳杆菌MT-1077。 用于从MT-1077乳杆菌中纯化细菌素的方法包括以下步骤：在MRS肉汤中培养MT-1077乳杆菌; 通过加入硫酸氢铵沉淀出乳杆菌MT-1077发酵培养液; 使蛋白沉淀物通过填充有羧甲基sepharose CL-6B的柱并用0.1M氯化钠洗脱吸附的蛋白质; 将洗脱液通过填充有苯基sepharose CL-4B的柱并用10％硫酸氢铵溶液洗脱吸附的蛋白质; 并通过HPLC纯化洗脱液
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13.发明公开신균주 스트렙토마이세스속 MT1882-V(KCTC 8800P)와 피에리시딘 A1의 인터루킨-2생성 억제제로서의 용도 失效使用新型菌株链霉菌属MT1882-V（KCTC 8800P）和Pierceine A1作为白细胞介素-2产生的抑制剂
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Patent Agency Ranking
公开(公告)号：KR100290532B1
公开(公告)日：2001-12-05
申请号：KR1019970040065
申请日：1997-08-22
Applicant: 한국과학기술연구원
IPC: C12N1/14
Abstract: PURPOSE: Provided are novel strain, fungus MT51005 (KCTC 8787P) and a composition for inhibiting the activity of phospholipase C which is an important enzyme for signal transfer in cell. The novel strain produces inhibitor of abnormal cell growth by inhibiting phospholipase C. CONSTITUTION: The composition for inhibiting the activity of phospholipase C comprises 2,4-dihydroxy-3-ethyl-6-(1'-methylpentene-1'-nyl)-benzaldehyde represented by the formula(1) and/or 2,4-dihydroxy-3-ethyl-6-(1'-methylpentene)-benzaldehyde represented by the formula(2) separated and purified from fungus MT51005 (KCTC 8787P).
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公开(公告)号：KR1020000026775A
公开(公告)日：2000-05-15
申请号：KR1019980044441
申请日：1998-10-19
Applicant: 한국과학기술연구원
IPC: A61K31/37
CPC classification number: C07D215/233 , A61K31/47
Abstract: PURPOSE: An angiotensin II receiving body binding inhibitor, which shows good inhibitive activity of the angiotensin II receiving body binding and good antagonistic action against vascular contraction induced by the angiotensin II, and a preparing method thereof are provided. CONSTITUTION: The angiotensin II receiving body binding inhibitor consists of a compound of following formula 1, where R represents aliphatic group having 5-20 carbon atoms, derivative thereof or salt thereof. The method comprises steps of: (i) extracting Evodiae Fructus with low class alcohol and then with acetate; and (ii) carrying out silica gel column chromatography, reverse phase low pressure column chromatography and high pressure liquid phase column chromatography on the extracted Evodiae Fructus. The pharmaceutical composition contains the compound of the formula 1 as an effective ingredient.
Abstract translation: 目的：提供血管紧张素II受体结合抑制剂，其具有良好的血管紧张素II受体结合的抑制活性和对血管紧张素II诱导的血管收缩的良好拮抗作用及其制备方法。 构成：血管紧张素II受体结合抑制剂由下式1的化合物组成，其中R表示具有5-20个碳原子的脂族基团，其衍生物或其盐。 该方法包括以下步骤：（i）用低级醇，然后用乙酸提取葫芦果; 和（ⅱ）对萃取的枸杞子进行硅胶柱层析，反相低压柱层析和高压液相柱层析。 药物组合物含有式1的化合物作为有效成分。
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公开(公告)号：KR100209787B1
公开(公告)日：1999-07-15
申请号：KR1019970015638
申请日：1997-04-25
Applicant: 한국과학기술연구원
IPC: C12N1/20
Abstract: 본 발명은 박테리오신을 생산하는 신규 락토코커스 속 미생물 및 이로부터 생산되는 신규 박테리오신에 관한 것으로, 본 발명에 따라 김치에서 분리된 락토코커스 속 MT-H559 균주는 항균성 펩티드인 박테리오신을 생산하며, 이로부터 생산된 신규 박테리오신은 넓은 범위의 pH 및 온도에서 안정하여 생물학적 식품 보존제로서 이용될 수 있다.
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17.发明公开신균주 곰팡이 MT51005(KCTC 8787P)와 이 균주로부터생산된 포스포리파제 C 활성저해용 조성물 失效一种新的菌株真菌MT51005（KCTC 8787P）和一种用于抑制菌株产生的磷脂酶C活性的组合物
公开(公告)号：KR1019990017226A
公开(公告)日：1999-03-15
申请号：KR1019970040065
申请日：1997-08-22
Applicant: 한국과학기술연구원
IPC: C12N1/14
Abstract: 본 발명은 신균주 곰팡이 MT51005(KCTC 8787P)와 포스포리파제 C 활성저해용 조성물에 관한 것으로서, 더욱 상세하게는 세포내 신호전달과정의 중요효소인 포스포리파제 C(Phospholipase C)의 활성을 저해하여 세포의 이상증식을 억제할 수 있는 물질을 생산하는 신규 곰팡이 균주 MT51005와, 곰팡이 MT51005의 배양액으로부터 분리 정제된 2,4-디하이드록시-3-에틸-6-(1'-메틸펜텐-1'-닐)-벤즈알데하이드, 2,4-디하이드록시-3-에틸-6-(1'-메틸펜텐)-벤즈알데하이드, 또는 이들 각각을 유효성분으로 함유한 포스포리파제 C 활성저해용 조성물에 관한 것이다.
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