公开(公告)号：KR100238103B1

公开(公告)日：2000-01-15

申请号：KR1019970040064

申请日：1997-08-22

Applicant: 한국과학기술연구원

Inventor： 안종석 ,  김보연 ,  민태익 ,  안순철 ,  함경수 ,  박찬선 ,  이현선 ,  최용경 ,  강대욱 ,  오원근 ,  고학룡

IPC: C12N1/20

Abstract: 본 발명은 신균주 스트렙토마이세스속(Streptomyces sp.) MT1882-V와 다음 화학식 1로 표시되는 피에리시딘 A1을 유효성분으로 함유하고 있는 면역억제제에 관한 것이다.

公开(公告)号：KR100378323B1

公开(公告)日：2003-03-29

申请号：KR1019990014356

申请日：1999-04-22

Applicant: 김용식 ,  정인원 ,  박주배 ,  한국과학기술연구원

Inventor： 김용식 ,  박주배 ,  안종석 ,  이현선 ,  정인원 ,  신철진 ,  오원근 ,  김시경 ,  강웅구 ,  홍성민

IPC: C07H13/08

CPC classification number: A61K31/704 ,  A61K36/69

Abstract: The present invention relates to psychotropic polygalasaponins and its extraction and isolation from the roots of Polygalae tenuifolia. The inhibitory activity of the extract from the roots of Polygalae tenuifolia on stereotyped behaviors and climbing behaviors induced by apomorphine was examined, and a single extract fraction that showed an activity particularly in behavioral pharmacology model was obtained from the roots of Polygalae tenuifolia. The object of the invention is therefore aimed at providing a new version of antipsychotic drug, polygalasaponic compound itself and a method to extract and isolate polygalasaponic compound from the roots of Polygalae Tenuifolia.

Abstract translation: 本发明涉及精神性多果皂苷及其从远志（Polygalae tenuifolia）根提取和分离。 研究了远志根部提取物对阿朴吗啡诱导的刻板行为和爬行行为的抑制活性，并从远志草的根部获得了一种特别在行为药理学模型中表现出活性的单一提取物部分。 因此，本发明的目的是提供一种新的抗精神病药物，多聚半乳糖化合物本身以及从远志藤根提取和分离多聚半乳糖化合物的方法。

公开(公告)号：KR100290532B1

公开(公告)日：2001-12-05

申请号：KR1019970040065

申请日：1997-08-22

Applicant: 한국과학기술연구원

Inventor： 안종석 ,  오원근 ,  민태익 ,  이현선 ,  안순철 ,  김보연 ,  강대욱 ,  박찬선 ,  고학룡

IPC: C12N1/14

Abstract: PURPOSE: Provided are novel strain, fungus MT51005 (KCTC 8787P) and a composition for inhibiting the activity of phospholipase C which is an important enzyme for signal transfer in cell. The novel strain produces inhibitor of abnormal cell growth by inhibiting phospholipase C. CONSTITUTION: The composition for inhibiting the activity of phospholipase C comprises 2,4-dihydroxy-3-ethyl-6-(1'-methylpentene-1'-nyl)-benzaldehyde represented by the formula(1) and/or 2,4-dihydroxy-3-ethyl-6-(1'-methylpentene)-benzaldehyde represented by the formula(2) separated and purified from fungus MT51005 (KCTC 8787P).

公开(公告)号：KR1020000066933A

公开(公告)日：2000-11-15

申请号：KR1019990014356

申请日：1999-04-22

Applicant: 김용식 ,  정인원 ,  박주배 ,  한국과학기술연구원

Inventor： 김용식 ,  박주배 ,  안종석 ,  이현선 ,  정인원 ,  신철진 ,  오원근 ,  김시경 ,  강웅구 ,  홍성민

IPC: C07H13/08

CPC classification number: A61K31/704 ,  A61K36/69

Abstract: PURPOSE: A psychotropic polygalasaponin based compound is provided which inhibits a stereotyped behavior and climbing behavior which are induced by apomorphine. CONSTITUTION: A psychotropic polygalasaponin is represented by formula 1 representation. An extraction method are characterized by comprising the following steps: (i)after grinding Polygalae Radix up to 70-230 mesh, extracting three times with 3-5 fold amount of alcohol solution; (ii)evaporating and condensing the filtrate at 70-76 cm Hg and at 60-70°C; (iii)after suspending the condensate in distilled water of 10 fold volume ratio, shaking the above thing by adding alcohol solvent of 1-2 fold and extracting three times and separating alcohol layer; (iv)condensing the above alcohol solvent fraction at 70-76 cmHg and at 60-70°C and obtaining an extract; (v)after suspending the above extract with distilled water of 50-100 fold adsorbing in resin HP-20 and draining active material with 70% methanol and condensing for obtaining an extract; (vi)after condensing the above extract with distilled water of 50-100 fold 2-3 times in common ratio, suspending homogeneously with same amount of distilled water and freeze-drying for obtaining a powder extract; (vii)dissolving the powder extract in ether and obtaining a precipitate;(viii)after saturating the precipitate with distilled water, passing through HP-20 resin and eluting an active site with methanol and adsorbing in silica gel and eluting active material by using ethyl acetate/methanol/water; (ix)adsorbing the active material in silica gel again and fractionating with mixed solvent of trichloro methane/methanol/ethanol/10%-acetic acid.

Abstract translation: 目的：提供一种精神分析多聚赖草皂苷的化合物，其抑制由阿朴吗啡诱导的定型行为和攀爬行为。 构成：精神分裂多糖分子由式1表示。 提取方法的特征在于包括以下步骤：（i）研磨多达70-230目后，用3-5倍量的醇溶液萃取三次; （ii）在70-76cm Hg和60-70℃下蒸发并冷凝滤液; （iii）将冷凝物悬浮在10倍体积比的蒸馏水中后，加入1-2倍的酒精溶剂振荡上述物质，萃取3次，分离醇层; （iv）将上述醇溶剂级分在70-76cmHg和60-70℃下冷凝并得到提取物; （v）将吸附在树脂HP-20中的50-100倍的蒸馏水悬浮在上述提取物上，并用70％甲醇排出活性物质并冷凝得到提取物; （vi）将上述提取物与常规比例为50-100倍的蒸馏水冷凝2-3次后，用相同量的蒸馏水均匀悬浮，冷冻干燥得到粉末提取物; （vii）将粉末提取物溶解在乙醚中并获得沉淀物;（viii）在用蒸馏水使沉淀物饱和后，通过HP-20树脂并用甲醇洗脱活性位点并吸附在硅胶中，并用乙基 乙酸乙酯/甲醇/水; （ix）再次将活性物质吸附在硅胶中，并用三氯甲烷/甲醇/乙醇/ 10％乙酸的混合溶剂进行分馏。

公开(公告)号：KR1020000026775A

公开(公告)日：2000-05-15

申请号：KR1019980044441

申请日：1998-10-19

Applicant: 한국과학기술연구원

Inventor： 이현선 ,  안종석 ,  민태익 ,  오원근 ,  안순철 ,  김보연 ,  강대욱 ,  박찬선 ,  고학룡 ,  이종화

IPC: A61K31/37

CPC classification number: C07D215/233 ,  A61K31/47

Abstract: PURPOSE: An angiotensin II receiving body binding inhibitor, which shows good inhibitive activity of the angiotensin II receiving body binding and good antagonistic action against vascular contraction induced by the angiotensin II, and a preparing method thereof are provided. CONSTITUTION: The angiotensin II receiving body binding inhibitor consists of a compound of following formula 1, where R represents aliphatic group having 5-20 carbon atoms, derivative thereof or salt thereof. The method comprises steps of: (i) extracting Evodiae Fructus with low class alcohol and then with acetate; and (ii) carrying out silica gel column chromatography, reverse phase low pressure column chromatography and high pressure liquid phase column chromatography on the extracted Evodiae Fructus. The pharmaceutical composition contains the compound of the formula 1 as an effective ingredient.

Abstract translation: 目的：提供血管紧张素II受体结合抑制剂，其具有良好的血管紧张素II受体结合的抑制活性和对血管紧张素II诱导的血管收缩的良好拮抗作用及其制备方法。 构成：血管紧张素II受体结合抑制剂由下式1的化合物组成，其中R表示具有5-20个碳原子的脂族基团，其衍生物或其盐。 该方法包括以下步骤：（i）用低级醇，然后用乙酸提取葫芦果; 和（ⅱ）对萃取的枸杞子进行硅胶柱层析，反相低压柱层析和高压液相柱层析。 药物组合物含有式1的化合物作为有效成分。

公开(公告)号：KR100209787B1

公开(公告)日：1999-07-15

申请号：KR1019970015638

申请日：1997-04-25

Applicant: 한국과학기술연구원

Inventor： 민태익 ,  안종석 ,  이헌주 ,  박찬선 ,  김승호 ,  이현선 ,  박용하 ,  주윤정 ,  박봉근

IPC: C12N1/20

Abstract: 본 발명은 박테리오신을 생산하는 신규 락토코커스 속 미생물 및 이로부터 생산되는 신규 박테리오신에 관한 것으로, 본 발명에 따라 김치에서 분리된 락토코커스 속 MT-H559 균주는 항균성 펩티드인 박테리오신을 생산하며, 이로부터 생산된 신규 박테리오신은 넓은 범위의 pH 및 온도에서 안정하여 생물학적 식품 보존제로서 이용될 수 있다.

公开(公告)号：KR1019990017226A

公开(公告)日：1999-03-15

申请号：KR1019970040065

申请日：1997-08-22

Applicant: 한국과학기술연구원

Inventor： 안종석 ,  오원근 ,  민태익 ,  이현선 ,  안순철 ,  김보연 ,  강대욱 ,  박찬선 ,  고학룡

IPC: C12N1/14

Abstract: 본 발명은 신균주 곰팡이 MT51005(KCTC 8787P)와 포스포리파제 C 활성저해용 조성물에 관한 것으로서, 더욱 상세하게는 세포내 신호전달과정의 중요효소인 포스포리파제 C(Phospholipase C)의 활성을 저해하여 세포의 이상증식을 억제할 수 있는 물질을 생산하는 신규 곰팡이 균주 MT51005와, 곰팡이 MT51005의 배양액으로부터 분리 정제된 2,4-디하이드록시-3-에틸-6-(1'-메틸펜텐-1'-닐)-벤즈알데하이드, 2,4-디하이드록시-3-에틸-6-(1'-메틸펜텐)-벤즈알데하이드, 또는 이들 각각을 유효성분으로 함유한 포스포리파제 C 활성저해용 조성물에 관한 것이다.