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公开(公告)号：KR1019950011612A
公开(公告)日：1995-05-15
申请号：KR1019930021925
申请日：1993-10-21
Applicant: 한국과학기술연구원
Abstract: 본 발명은 음이온 교환 수지를 사용하여 분리가능한 신규의 고구마 유래 퍼옥시다제, 상기 퍼옥시다제를 효율적으로 대량 생산할 수 있는 고구마 배양세포주, 상기 배양세포주를 배양하여 퍼옥시다제를 대량 생산하는 방법 및 상기 방법으로 생산된 퍼옥시다제를 배양세포로부터 효과적으로 정제하는 방법에 관한 것이다.
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公开(公告)号：KR1019940006323B1
公开(公告)日：1994-07-16
申请号：KR1019910007225
申请日：1991-05-03
Applicant: 한국과학기술연구원
IPC: A61K36/24
Abstract: This invention relates to an extraction method of indole alkaloid from Catharanthus roseus using high pressure and high density carbon dioxide solvent. The method comprises: a) placing Catharanthus in contact with 15-100 deg.C carbon dioxide under 30-500 atm and extracting indole alkaloid; and b) separating indole alkaloid and solvent at 1-10 atm and -30 - 20 deg.C. Using this extration method, there is no residual solvent problem.
Abstract translation: 本发明涉及使用高压和高密度二氧化碳溶剂从长春花提取吲哚生物碱的提取方法。 该方法包括：a）将长春花与30-100度二氧化碳置于30-500大气压下，提取吲哚生物碱; 和b）在1-10atm和-30-20℃下分离吲哚生物碱和溶剂。 使用这种方法，没有残留的溶剂问题。
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Patent Agency Ranking
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公开(公告)号：KR1019970002667B1
公开(公告)日：1997-03-07
申请号：KR1019930021925
申请日：1993-10-21
Applicant: 한국과학기술연구원
Abstract: Disclosed is the mass production process of the peroxidase using anion exchange resin, which is possible to separate and produce the sweet-potato peroxidase in large quantities by culturing a sweet potato culture cell strain. The process comprises the following steps of; A callus is induced by cutting apical meristem from a new shoot of the potato, and then culturing it on a solid medium containing 2,4-dichlorophenoxy acetic acid. A cell strain having a high peroxidase activity and a good cell proliferation in the induced callus is separated to obtain the mass producing cell strain by culturing it again on the same medium. The mass producing cell strain, which can produce the peroxidase, is cultured in a nutrient medium under a suitable suspension condition. An ethylene or abscisic acid are added to a culture fluid on the way of cultivation, wherein improving the peroxidase productivity remarkably.
Abstract translation: 公开了使用阴离子交换树脂的过氧化物酶的大量生产方法，其可以通过培养甘薯培养细胞株分离并产生大量的甘薯过氧化物酶。 该过程包括以下步骤： 通过从马铃薯的新芽切割顶端分生组织诱导愈伤组织，然后在含有2,4-二氯苯氧乙酸的固体培养基上培养。 分离出在诱导愈伤组织中具有高过氧化物酶活性和良好细胞增殖的细胞株，通过在同一培养基上再次培养来获得批量生产细胞株。 可以在合适的悬浮条件下在营养培养基中培养可产生过氧化物酶的大量生产细胞株。 在培养途中向培养液中加入乙烯或脱落酸，其中显着提高过氧化物酶的生产。
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公开(公告)号：KR1019910007612B1
公开(公告)日：1991-09-28
申请号：KR1019900000246
申请日：1990-01-10
Applicant: 한국과학기술연구원
Abstract: A process for producing human insulin in a tobacco tissue comprises: (a) inserting human proinsulin gene into the vector pBKS-1 to obtain the recombinant plasmid DNA; (b) inducing and transforming it into the Agrobacterium tumefaciens LBA 4404 (I), and separating the transformed (I); (c) adding the transformed (I) into the tobacco leaves callus and culturing at 25 deg.C for 1- 2days in the dark place; (d) washing it with MS medium and culturing in the selective medium contg. kanamycin and carbenicillin; (e) culturing it to obtain the tobacco tissue KCTC 84876P, producing human insulin.
Abstract translation: 在烟草组织中生产人胰岛素的方法包括：（a）将人胰岛素原基因插入载体pBKS-1中以获得重组质粒DNA; （b）将其诱导并转化到根癌土壤杆菌LBA 4404（I）中，并分离转化的（I）; （c）将转化的（I）加入到烟草叶愈伤组织中，并在黑暗的地方在25℃下培养1-2天; （d）用MS培养基洗涤并在选择性培养基中培养。 卡那霉素和羧苄青霉素; （e）培养以获得产生人胰岛素的烟草组织KCTC 84876P。
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