-
公开(公告)号：KR1019910007612B1
公开(公告)日：1991-09-28
申请号：KR1019900000246
申请日：1990-01-10
Applicant: 한국과학기술연구원
Abstract: A process for producing human insulin in a tobacco tissue comprises: (a) inserting human proinsulin gene into the vector pBKS-1 to obtain the recombinant plasmid DNA; (b) inducing and transforming it into the Agrobacterium tumefaciens LBA 4404 (I), and separating the transformed (I); (c) adding the transformed (I) into the tobacco leaves callus and culturing at 25 deg.C for 1- 2days in the dark place; (d) washing it with MS medium and culturing in the selective medium contg. kanamycin and carbenicillin; (e) culturing it to obtain the tobacco tissue KCTC 84876P, producing human insulin.
Abstract translation: 在烟草组织中生产人胰岛素的方法包括：（a）将人胰岛素原基因插入载体pBKS-1中以获得重组质粒DNA; （b）将其诱导并转化到根癌土壤杆菌LBA 4404（I）中，并分离转化的（I）; （c）将转化的（I）加入到烟草叶愈伤组织中，并在黑暗的地方在25℃下培养1-2天; （d）用MS培养基洗涤并在选择性培养基中培养。 卡那霉素和羧苄青霉素; （e）培养以获得产生人胰岛素的烟草组织KCTC 84876P。
-
-
-
公开(公告)号：KR1019910007611B1
公开(公告)日：1991-09-28
申请号：KR1019890017861
申请日：1989-12-04
Applicant: 한국과학기술연구원
IPC: C12N15/31
Abstract: A gene for coding ice nucleating protein is prepd. by culturing Pseudomonas syringae AL, KCTC 1832, separating its chromosomal DNA, treating with restriction enzyme Bgl II/EcoRI, applicating in agarose gel electrophoresis, moving nytran membrane, hybridizing with 32P-oligonucleotide, 5'- CCGTAACCGGCGGTCAACGTGC3' to obtain the selected DNA fragment, inserting into the Bam HI/EcoRI site of vector pBR 322 to obtain the recombinant DNA, amplifying in E. coli, separating the plasmid DNA, treating with Bgl II/ EcoRI to obtain the ice nucleus activating gene clone pGINP-1, inserting to the E. coli KCTC 8475 (I). The (I) is useful for prevention of freezing and/or frost damage of frozen food prodn. and preservation.
Abstract translation: 用于编码冰核蛋白的基因是制备的。 通过培养丁香假单胞菌AL，KCTC 1832，分离其染色体DNA，用限制酶Bgl II / EcoRI处理，应用于琼脂糖凝胶电泳，移动nttran膜，与32P-寡核苷酸杂交，5'-CCGTAACCGGCGGTCAACGTGC3'获得所选择的DNA片段 插入载体pBR 322的Bam HI / EcoRI位点，得到重组DNA，扩增大肠杆菌，分离质粒DNA，用Bgl II / EcoRI处理，得到冰核激活基因克隆pGINP-1，插入 大肠杆菌KCTC 8475（I）。 （I）可用于预防冷冻食品的冻结和/或霜冻损伤。 并保存。
-
公开(公告)号：KR1019930009562B1
公开(公告)日：1993-10-07
申请号：KR1019910007224
申请日：1991-05-03
Applicant: 한국과학기술연구원
IPC: C12N15/82
Abstract: The method for producing human interleukin-2 protein in a tobacco tissue comprises: (a) inserting human interleukin-2 gene into a vector pBKS-1 to obtain a recombinant plasmid DNA; (b) transforming it into Agrobacterium tumefaciens; (c) adding the transformants into tobacco leaves callus and culturing at 25 deg.C for 1-2 days in a dark place; (d) washing it with MS medium and culturing in a selective medium containing kanamycin and carbenicillin; (e) culturing it to obtain a tobacco tissue (KCTC 8499P), producing human interleukin-2 protein.
Abstract translation: 在烟草组织中产生人白介素-2蛋白的方法包括：（a）将人白细胞介素-2基因插入载体pBKS-1中以获得重组质粒DNA; （b）将其转化为根癌土壤杆菌; （c）将转化体加入烟草叶愈伤组织，并在黑暗的地方在25℃下培养1-2天; （d）用MS培养基洗涤并在含有卡那霉素和羧苄青霉素的选择培养基中培养; （e）培养以获得烟草组织（KCTC 8499P），产生人白细胞介素-2蛋白。
-
-
-
-
Search Results
10
records
(took 0.017 seconds)
