公开(公告)号：WO2021255593A2

公开(公告)日：2021-12-23

申请号：PCT/IB2021/055122

申请日：2021-06-10

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： YOUNG, Alexi J. ,  HALVERSON, Kurt J. ,  SWANSON, Steven P. ,  BRUTINEL, Evan D. ,  NELSON, Caleb T.

IPC: C12M1/12 ,  C12M1/00 ,  C12M23/04 ,  C12M23/20 ,  C12M23/22 ,  C12M23/26 ,  C12M23/28 ,  C12M23/38 ,  C12M25/06

Abstract: A device for growing microorganisms. The device can include a self-supporting, water-proof substrate having an inner-facing surface and an outer-facing surface; a cover sheet having an inner-facing surface and an outer-facing surface, the cover sheet adhered to at least a portion of the substrate; a microstructured surface on the inner-facing surface of the coversheet, wherein the microstructured surface comprises a plurality of microstructures; a substantially dry, gelling agent and a substantially dry, microbial growth nutrient composition disposed on the microstructured surface of the microstructured film or on a portion of the inner-facing surface of the substrate.

公开(公告)号：WO2018125811A1

公开(公告)日：2018-07-05

申请号：PCT/US2017/068233

申请日：2017-12-22

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： BRUTINEL, Evan D. ,  YOUNG, Alexi J.

IPC: C12M1/16 ,  C12M1/34

Abstract: Devices for microbial detection of microorganisms are provided including a body member including a substrate having a first major surface and a second major surface. The device further includes a first adhesive composition adhered to a portion of the first major surface. A number of particles of a substantially dry first microbial growth nutrient composition are distributed in the first adhesive composition, and a cold-water-soluble first hydrogel-forming composition is adhered to the first adhesive composition. The device also includes a cover sheet attached to the body member, where the cover sheet includes a first major surface facing the body member. Devices including a water-proof pouch, further comprising a porous membrane filter are also provided. Methods for detecting and enumerating at least one microorganism in a sample using the devices are additionally provided.

公开(公告)号：WO2017112564A1

公开(公告)日：2017-06-29

申请号：PCT/US2016/067437

申请日：2016-12-19

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： BRUTINEL, Evan D. ,  RULE, Joseph D. ,  JANOSKI, Jonathan E. ,  SCHULTZ, Anthony F.

IPC: B01L3/00

CPC classification number: B01L3/50853 ,  B01L2300/041 ,  B01L2300/044 ,  B01L2300/0896 ,  B01L2300/123

Abstract: Sample partitioning devices and methods of making and using the same are described. A sample partitioning device includes a first film having an array of discrete stems each extending from a first major surface thereof, and a second film having an array of discrete wells formed into a second major surface thereof. The stems of the first film and the wells of the second film are mated with each other. The mated stems and wells are separable from each other, and during the removal of the stems from the wells, one or more voids are created inside the wells to suction an aqueous test sample into the wells.

Abstract translation: 描述样本分区设备及其制作和使用方法。 样品分隔装置包括第一膜和第二膜，所述第一膜具有各自从其第一主表面延伸的离散杆阵列，所述第二膜具有形成在其第二主表面中的离散阱阵列。 第一层膜的茎和第二层膜的孔相互配合。 配合的茎和孔彼此可分离，并且在从孔中移除茎的过程中，在孔内产生一个或多个空隙以将含水测试样品吸入孔中。

公开(公告)号：WO2016149235A1

公开(公告)日：2016-09-22

申请号：PCT/US2016/022411

申请日：2016-03-15

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： RAJAGOPAL, Raj ,  KSHIRSAGAR, Manjiri T. ,  BRUTINEL, Evan D. ,  AYSTA, James E.

IPC: G01N1/10 ,  B01D46/00 ,  C12M1/26 ,  C12Q1/24 ,  C12M1/12 ,  C12Q1/04 ,  C12M1/34 ,  G01N1/40

CPC classification number: C12Q1/24 ,  B01D46/00 ,  C12M1/12 ,  C12M1/26 ,  C12M1/34 ,  C12Q1/04 ,  G01N1/10 ,  G01N1/405 ,  G01N2001/4088

Abstract: Methods of detecting microorganisms in a fluid sample are provided. The method includes providing a nonwoven article, providing a fluid sample suspected of containing at least one microorganism strain or target cellular analyte, and contacting the fluid sample with the nonwoven article such that at least a portion of the at least one microorganism strain or target cellular analyte is bound to the nonwoven article. The nonwoven article includes a fibrous porous matrix and a concentration agent particles enmeshed in the fibrous porous matrix. The method further includes placing the microorganism strain- or target cellular analyte-bound nonwoven article in contact with at least one detection reagent and detecting the presence of the bound microorganism strain or bound target cellular analyte. Devices and kits containing the devices, for contacting a fluid sample with a nonwoven article, are also provided. The device includes a sample container, a filter holder, a nonwoven article, and an adaptor configured to interface the filter holder with a receptacle.

Abstract translation: 提供了检测流体样品中的微生物的方法。 该方法包括提供非织造制品，提供怀疑含有至少一种微生物菌株或靶细胞分析物的流体样品，并使流体样品与非织造制品接触，使得至少一部分微生物菌株或靶细胞 分析物结合到非织造制品上。 非织造制品包括纤维多孔基质和包含在纤维状多孔基质中的浓缩剂颗粒。 该方法还包括将微生物菌株或靶细胞分析物结合的非织造制品置于与至少一种检测试剂接触并检测结合的微生物菌株或结合的目标细胞分析物的存在。 还提供了包含用于使流体样品与非织造制品接触的装置的装置和试剂盒。 该装置包括样品容器，过滤器保持器，非织造制品和被配置为使过滤器保持器与容器接合的适配器。

公开(公告)号：WO2016028839A1

公开(公告)日：2016-02-25

申请号：PCT/US2015/045795

申请日：2015-08-19

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： BRUTINEL, Evan D. ,  BJORK, Jason W., ,  STANENAS, Adam J.

IPC: C12M1/00 ,  C12M1/34 ,  C12Q1/04 ,  G01N33/52

CPC classification number: C12M33/00 ,  C12M23/04 ,  C12M23/20 ,  C12M25/06 ,  C12M37/04 ,  C12M41/36 ,  C12Q1/04

Abstract: The present disclosure provides a culture device for enumerating colonies of sulfate-reducing microorganisms. The device includes a body having a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed therebetween. The growth compartment has a perimeter and an opening. A portion of the perimeter is defined by a waterproof seal. The portion can include >50% of the perimeter. Disposed in the growth compartment are a dry cold water-soluble gelling agent, a dry culture medium selected to facilitate growth of a sulfate-reducing bacterium or indicator reagent for detecting hydrogen sulfide production by a sulfate-reducing bacterium, and a dry first oxygen-scavenging reagent.

Abstract translation: 本公开提供了一种用于计数硫酸盐还原微生物菌落的培养装置。 该装置包括具有防水基座的主体，附接到基座的防水覆盖片，以及设置在其间的生长室。 生长室具有周长和开口。 周边的一部分由防水密封限定。 该部分可以包括> 50％的周长。 在生长室中设置干燥的冷水溶性胶凝剂，选择用于促进硫酸盐还原细菌的生长的干燥培养基或用于检测硫酸盐还原细菌产生硫化氢的指示剂，以及干燥的第一氧 - 清除剂。

公开(公告)号：WO2019229600A1

公开(公告)日：2019-12-05

申请号：PCT/IB2019/054305

申请日：2019-05-23

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： YOUNG, Alexi J. ,  KRUSE, John M. ,  BROWN, Christopher M. ,  PETERSON, Donald G. ,  BRUTINEL, Evan D.

IPC: B01L3/00 ,  C12M1/34 ,  C12Q1/04

Abstract: A microbial detection device and methods of use are provided. The device comprises a water-proof pouch that includes a first wall portion, a second wall portion, and a porous membrane filter disposed in the pouch between the first and second wall portions. The filter membrane divides the pouch into first and second compartments. A dry, cold water-soluble gelling agent is adhered to the pouch in the first compartment and an absorbent pad is disposed in the second compartment. A release liner removably adhered to the first adhesive layer.

公开(公告)号：WO2017106098A1

公开(公告)日：2017-06-22

申请号：PCT/US2016/066165

申请日：2016-12-12

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： VANDRE, Eric A. ,  STAY, Matthew S. ,  SMITH, Matthew R. D. ,  GILMAN, Ann M. ,  DODDS, Shawn C. ,  DABLE, Jon E. ,  BRUTINEL, Evan D.

IPC: B41M1/12 ,  B41M1/22 ,  B41N1/24

CPC classification number: B41M1/12 ,  B41M1/22

Abstract: Methods for using a hollow, rotating stencil roll to deposit flowable dry powder particles onto a moving substrate and to attach the particles to the substrate.

Abstract translation: 使用中空的旋转模版辊将可流动的干粉颗粒沉积到移动的基材上并将颗粒附着到基材上的方法。

公开(公告)号：WO2017058737A1

公开(公告)日：2017-04-06

申请号：PCT/US2016/053863

申请日：2016-09-27

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： BRUTINEL, Evan D. ,  BJORK, Jason W. ,  STANENAS, Adam J.

IPC: C12M1/32 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/04

CPC classification number: C12M23/12 ,  C12M23/38 ,  C12M41/34 ,  C12M41/36 ,  C12Q1/04 ,  C12Q1/24

Abstract: In one aspect, the present disclosure provides a device. The device includes a body comprising a waterproof base, a waterproof coversheet attached to the base, and a channel disposed between the base and the coversheet. The channel has a perimeter and an opening that provides liquid access to the channel. A portion of the perimeter is defined by a waterproof seal. A dry first oxygen-scavenging reagent and an indicator reagent for detecting sulfate reduction by a sulfate-reducing bacterium are disposed in the device between the base and the coversheet. The waterproof base comprises a plurality of open microcompartment structures facing the coversheet.

Abstract translation: 一方面，本公开提供了一种装置。 该装置包括主体，该主体包括防水基座，附接到基座的防水覆盖片以及设置在基座和盖板之间的通道。 通道具有周边和开口，可以提供液体进入通道。 周边的一部分由防水密封限定。 干燥的第一氧气清除试剂和用于通过硫酸盐还原细菌检测硫酸盐还原的指示剂被设置在基底和覆盖片之间的装置中。 防水底座包括面向盖板的多个开放微型隔室结构。

公开(公告)号：WO2017019345A1

公开(公告)日：2017-02-02

申请号：PCT/US2016/042712

申请日：2016-07-18

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： BRUTINEL, Evan D. ,  YOUNG, Alexi J. ,  THOMPSON, Delton R., Jr.

IPC: C12M1/34 ,  C12Q1/04

CPC classification number: C12Q1/04 ,  C12M1/34 ,  C12M23/26 ,  C12M23/34 ,  C12M41/36

Abstract: A microbial detection device and methods of use are provided. The device comprises a water-proof pouch that includes a first wall portion, a second wall portion, and a porous membrane filter disposed in the pouch between the first and second wall portions. The filter membrane divides the pouch into first and second compartments. A dry, cold water-soluble gelling agent is adhered to the pouch in the first compartment and an absorbent pad is disposed in the second compartment. A sealable sample port provides access to deposit a liquid into the first compartment.

Abstract translation: 提供微生物检测装置和使用方法。 该装置包括防水袋，其包括设置在第一和第二壁部之间的袋中的第一壁部分，第二壁部分和多孔膜过滤器。 过滤膜将袋分成第一和第二隔室。 干燥的冷水溶性胶凝剂粘附到第一隔室中的袋子上，并且吸收垫设置在第二隔室中。 可密封的样品端口提供进入以将液体沉积到第一隔室中。

公开(公告)号：WO2016176183A1

公开(公告)日：2016-11-03

申请号：PCT/US2016/029318

申请日：2016-04-26

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： BRUTINEL, Evan D. ,  FORESTER, Mary B.

IPC: C12M1/34 ,  C12M1/12 ,  C12Q1/04

CPC classification number: C12M1/045 ,  C12M1/005 ,  C12M1/007 ,  C12M1/14 ,  C12M23/14 ,  C12M23/22 ,  C12M25/02 ,  C12M25/06 ,  C12M41/34 ,  C12M41/36 ,  C12N1/20 ,  C12Q1/04

Abstract: A device for culturing anaerobic microorganisms is provided. The device comprises a body comprising a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed between the base and the coversheet. The growth compartment has a perimeter and an opening that provides liquid access to the growth compartment. A portion of the perimeter is defined by a waterproof seal. The portion includes >50% of the perimeter. A dry cold water-soluble gelling agent is adhered to the base in the growth compartment. A dry first oxygen-scavenging reagent is disposed in the growth compartment.

Abstract translation: 提供了一种用于培养厌氧微生物的装置。 该装置包括主体，其包括防水基座，附接到基座的防水覆盖片，以及设置在基座和覆盖片之间的生长室。 生长室具有周边和开口，其提供液体进入生长室。 周边的一部分由防水密封限定。 该部分包括> 50％的周长。 干燥的冷水溶性胶凝剂粘附到生长室中的底部。 干燥的第一氧气清除试剂设置在生长室中。