METHODS FOR TRANSFORMING YEAST
    14.
    发明专利

    公开(公告)号:NZ587766A

    公开(公告)日:2013-06-28

    申请号:NZ58776609

    申请日:2009-03-03

    Applicant: ABBOTT LAB

    Abstract: NZ 587766 Disclosed is a method of preparing a yeast library by electroporation of yeast cells, the method comprising the steps of: incubating the yeast cells in a solution comprising lithium acetate (LiAc) and dithiothreitol (DTT); providing a suspension comprising vector DNA, insert DNA, yeast cells, 0.1 to 10 M sorbitol, and 0.1 to 10 mM CaCl2 or MgCl2; and electroporating the suspension at 0.5kV/cm to greater than 12.5kV/cm with a capacitance of about 10 to about 50 mu ?F.

    METHODS FOR TRANSFORMING YEAST
    15.
    发明专利

    公开(公告)号:SG188846A1

    公开(公告)日:2013-04-30

    申请号:SG2013016084

    申请日:2009-03-03

    Applicant: ABBOTT LAB

    Abstract: Methods for Transforming Yeast AbstractThis invention is directed to the transformtion of yeast, and mutants thereof, by electroporation, which result in stably transformed yeast host cells that express recombinant products. This invention also is directed to transformed yeast cells and libraries.Figure I

Patent Agency Ranking