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    METHOD FOR CREATING POLYNUCLEOTIDE MOLECULES
    17.
    发明专利
    METHOD FOR CREATING POLYNUCLEOTIDE MOLECULES 未知

    公开(公告)号：CA2485218A1

    公开(公告)日：2003-12-04

    申请号：CA2485218

    申请日：2003-05-21

    Applicant: BASF AG

    Inventor： HAUER BERNHARD ,  MATUSCHEK MARKUS

    IPC: C12N15/09 ,  C12N15/10 ,  C12P19/34

    Abstract: The invention relates to methods for creating polynucleotide molecules with modified characteristics, said methods running through a cycle comprising th e following steps:(a) provision of a double-stranded polynucleotide molecule o r a population of double-stranded polynucleotide molecules, the individual polynucleotides of said population having at least one homologous sequence part and at least one heterologous sequence part,(b) creation of single-stra nd breaks in the double-stranded polynucleotide molecules, (c) nucleolytic degradation in the 5'.fwdarw. 3' direction starting from the single-strand breaks with simultaneous new synthesis in the 5' .fwdarw. 3' direction and shifting of the single-strand breaks in the direction of the 3' end, (d) production of single-stranded polynucleotide molecules, (e) production of partially double-stranded polynucleotide molecules of the single-stranded polynucleotide molecules provided in step (d), (f) template-directed nucleic acid synthesis starting from the partially double-stranded polynucleotide molecules produced in step (e), whereby steps (b) and (c) can be carried out in succession or simultaneously.

    New bacterial lipase mutants, useful for enantioselective conversion, e.g. acylation of alcohols, have increased specific activity
    18.
    发明专利
    New bacterial lipase mutants, useful for enantioselective conversion, e.g. acylation of alcohols, have increased specific activity 未知

    公开(公告)号：DE10205444A1

    公开(公告)日：2003-08-21

    申请号：DE10205444

    申请日：2002-02-08

    Applicant: BASF AG

    Inventor： MATUSCHEK MARKUS ,  STUERMER RAINER ,  HAUER BERNHARD ,  KLEBE GERHARD ,  BARCOLA MARCO

    IPC: C07K14/21 ,  C12N1/21 ,  C12N9/20 ,  C12N15/55

    Abstract: Lipase variants (I) prepared by performing at least one amino acid substitution in a starting lipase (II), of lipase homology families I.1 or I.2 are new. The amino acid substitution is at a position corresponding to positions 17, 29, 30, 52, 86, 117, 122, 160, 163, 167, 265, 266, 286 or 289 of a 319 residue prototype lipase sequence (S1), given in the specification. Independent claims are also included for the following: (1) nucleic acid (II) that encodes (I); (2) nucleic acid constructs that contain (II); (3) host organisms transformed with the constructs of (2); (4) preparing (I) by culturing the organisms of (3); (5) lipase formulations containing at least one (I); (6) enzyme-catalyzed or enantioselective conversion of substrates using (I); and (7) preparing optically active compounds by enantioselective reaction of a stereoisomeric mixture or racemate of a lipase-sensitive substrate, using (I), then separation of the reaction mixture.

    New bacterial lipase mutants, useful for enantioselective conversion, e.g. acylation of alcohols, have increased specific activity
    19.
    发明专利
    New bacterial lipase mutants, useful for enantioselective conversion, e.g. acylation of alcohols, have increased specific activity 未知

    公开(公告)号：DE10151292A1

    公开(公告)日：2003-04-30

    申请号：DE10151292

    申请日：2001-10-22

    Applicant: BASF AG

    Inventor： MATUSCHEK MARKUS ,  STUERMER RAINER ,  HAUER BERNHARD ,  KLEBE GERHARD ,  BOCOLA MARCO

    IPC: C12N9/20 ,  C12N15/55 ,  C07K14/21

    Abstract: Lipase variants (I) prepared by performing at least one amino acid substitution in a starting lipase (II), of lipase homology families I.1 or I.2 are new. The amino acid substitution is at a position corresponding to positions 17, 29, 30, 52, 86, 117, 122, 160, 163, 167, 265, 266, 286 or 289 of a 319 residue prototype lipase sequence (S1), given in the specification. Independent claims are also included for the following: (1) nucleic acid (II) that encodes (I); (2) nucleic acid constructs that contain (II); (3) host organisms transformed with the constructs of (2); (4) preparing (I) by culturing the organisms of (3); (5) lipase formulations containing at least one (I); (6) enzyme-catalyzed or enantioselective conversion of substrates using (I); and (7) preparing optically active compounds by enantioselective reaction of a stereoisomeric mixture or racemate of a lipase-sensitive substrate, using (I), then separation of the reaction mixture.

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