A PROCESS FOR PRODUCTION OF A PROTEIN OF INTEREST IN A MICROBIAL HOST ORGANISM

    公开(公告)号:CA2933975C

    公开(公告)日:2022-06-21

    申请号:CA2933975

    申请日:2014-12-12

    Applicant: BASF SE

    Abstract: A process for production of a protein of interest in a microbial host organism comprising the steps of: a) constructing a vector comprising a gene for the protein of interest and a functional frr gene and no antibiotic resistance gene, b) transforming a host organism having an inactivated chromosomal frr gene with the vector obtained in a), c) culturing the transformed host organism obtained in b) under conditions allowing the expression of the gene of interest in the host organism and d) isolating the protein of interest.

    PROCESS FOR PREPARATION OF MICROCAPSULES

    公开(公告)号:CA3012160A1

    公开(公告)日:2017-08-17

    申请号:CA3012160

    申请日:2017-02-02

    Applicant: BASF SE

    Abstract: Process for the preparation of microcapsules comprising the steps (a) preparation of an aqueous biphasic system by mixing (i) component (a1 ) comprising a component A selected from the group of polymers consisting of polyethylene glycol vinyl acetate comb polymers, polycarboxylates, polyethers, polyaspartates, polyvinylpyrrolidone, polyamines, and polylysine; wherein component (a1 ) is a monophasic system at 23°C, and forms a monophasic system at 23°C if mixed with water in the range of from 1 :99 to 99:1 by weight, and (ii) component (a2) containing water and a water-soluble component B, wherein water-soluble component B is different from component A, and wherein (a2) is a monophasic system at 23°C, and (iii) at least one monomer (a3), and (iv) optionally at least one initiator (a4), wherein (a1 ), (a2), (a3), and (a4) can be mixed together in any order or simultaneously, followed by (b) optionally shearing of the biphasic system to form an emulsion, and (c) polymerization of monomer (a3).

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