Abstract:
A POLAROGRAPHIC SENSOR COMPRISING A PAIR OF ELECTRODES JOINED BY AN ELECTOLYTE WITH INNER AND OUTER MEMBRANES SEPARATING THE ELECTRODE AND ELECTROLYTE FROM THE SAMPLE MEDIUM. THE OUTER MEMBRANE IS PERMEABLE TO THE GAS BEING DETERMINED AND IMPERMEABLE TO ELECTROLYTE WHILE THE INNER MEMBRANE HAS AN OPENING THEREIN WHICH OVERLIES THE SENSING ELECTRODE OF THE SENSOR. THIS INNER MEMBRANE IS SUBSTANTIALLY LESS PERMEABLE TO THE GAS BEING ANALYZED THAN THE OUTER MEMBRANE AND IS HYDROPHILIC SO AS TO RETAIN A FILM OF ELECTROLYTE BETWEEN THE OUTER MEMBRANE AND THE ELECTRODES. PREFERABLY THE OUTER MEMBRANE IS FORMED OF POLYTETRAFLUOROETHYLENE AND THE INNER MEMBRANE OF POLYETHYLENE TEREPHTHALATE (MYLAR).
Abstract:
Disclosed herein is a methodology for analyzing isoenzymes using capillary zone electrophoresis ("CZE") techniques. Briefly, an isoenzyme-containing sample and a substrate capable of being catalyzed by said isoenzyme into a reaction product are introduced into a capillary column comprising a buffer. Most preferably, the buffer contains the substrate prior to introduction of the sample into such substrate-buffer. CZE separation techniques are applied to the column such that the isoenzymes are separated from each other into discrete zones. The separation techniques are terminated such that product is rapidly generated by the catalytic conversion of substrate by the isoenzymes, and accumulated, within each discrete zone, followed by detection of product. Information regarding the relative distribution of the isoenzymes can be derived from the relative distribution of the product.
Abstract:
A method for perforating a sheet material provides for a perforating die located intermediate two orbital rollers. The two orbital rollers move in orbital paths in the same orbital direction at the same speed and in positions which are 180 degrees apart. Each roller acts as a temporary storage input and output in opposite phase from each other so that incoming sheet material is alternately stored and dispensed as a loop on the orbital roller. Since the rollers are out of phase, this permits a segment of the sheet material to be stopped intermediate the orbital rollers so that the perforating die can act on the stopped segment.