公开(公告)号：CA2825651C

公开(公告)日：2020-03-24

申请号：CA2825651

申请日：2012-02-01

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J

IPC: C01B25/00 ,  C01B25/32 ,  C07K1/16 ,  C07K16/00

Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example after a flow-through collection of a target and prior to cleaning the chromatography solid support. In some embodiments, the method comprises, (a) contacting a sample comprising the target molecule to an apatite solid surface thereby flowing the target molecule past the apatite solid surface; (b) neutralizing the apatite solid surface by contacting the apatite solid surface with a sufficient concentration and volume of an alkaline hydroxide; and (c) cleaning the apatite solid surface.

公开(公告)号：CA2825651A1

公开(公告)日：2012-08-09

申请号：CA2825651

申请日：2012-02-01

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J

IPC: C01B25/00 ,  C01B25/32 ,  C07K1/16 ,  C07K16/00

Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example after a flow-through collection of a target and prior to cleaning the chromatography solid support. In some embodiments, the method comprises, (a) contacting a sample comprising the target molecule to an apatite solid surface thereby flowing the target molecule past the apatite solid surface; (b) neutralizing the apatite solid surface by contacting the apatite solid surface with a sufficient concentration and volume of an alkaline hydroxide; and (c) cleaning the apatite solid surface.

公开(公告)号：DE4119203A1

公开(公告)日：1992-02-13

申请号：DE4119203

申请日：1991-06-11

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J ,  TAYLOR MICHAEL A

IPC: B01D15/08 ,  C07K1/14 ,  C07K1/16 ,  C07K16/06 ,  G01N30/38 ,  G01N30/60

公开(公告)号：CA2787009C

公开(公告)日：2018-04-03

申请号：CA2787009

申请日：2011-01-13

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J ,  SNYDER MARK A

IPC: B01D15/08

Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution. Specifically, provided are methods for purifying a target molecule in a sample, comprising contacting a sample comprising the target molecule to an apatite solid surface, contacting the solid surface comprising the adsorbed target molecule with a solution comprising a basic amino compound and alkali metal ion or alkali earth ion; or a sulphonated amine compound and alkali metal ion or alkali earth ion, and eluting the target molecule from the solid support by contacting the solid support with a different solution.

公开(公告)号：CA2594604C

公开(公告)日：2012-10-02

申请号：CA2594604

申请日：2006-01-17

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J

IPC: B01D15/08

Abstract: A chromatography cartridge with a packed bed is manufactured from an inner and outer chamber (11), the inner chamber being in an insert (12) that is slidable into the outer chamber (11) with openings in each such that a slurry that is originally placed in the interior of the outer chamber (11) as the parts are being pressed into each other. During the assembly, the particles in the slurry are compressed to fill the smaller volume of the inner chamber and excess liquid is discharged through ports in both chambers. The construction allows for a highly precise and reproducible column filling process with a minimum of steps and operator intervention and thereby low cost and reduced error.

公开(公告)号：CA2594604A1

公开(公告)日：2006-08-24

申请号：CA2594604

申请日：2006-01-17

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J

IPC: B01D15/08

Abstract: A chromatography cartridge with a packed bed is manufactured from an inner and outer chamber, the inner chamber being in an insert that is slidable into the outer chamber with openings in each such that a slurry that is originally placed in the interior of the outer chamber flows into the inner chamber as the parts are being pressed into each other. During the assembly, the particles in the slurry are compressed to fill the smaller volume of the inner chamber and excess liquid is discharged through ports in both chambers. The construction allows for a highly precise and reproducible column filling process with a minimum of steps and operator intervention and thereby low cost and reduced error.

公开(公告)号：IT1251262B

公开(公告)日：1995-05-05

申请号：ITRM910595

申请日：1991-08-05

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J ,  TAYLOR MICHAEL A

IPC: B01D15/08 ,  C07K1/14 ,  C07K1/16 ,  C07K16/06 ,  G01N30/38 ,  G01N30/60 ,  B01D

公开(公告)号：EP2524215A4

公开(公告)日：2014-08-20

申请号：EP11733384

申请日：2011-01-13

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J ,  SNYDER MARK A

IPC: B01D15/38 ,  B01D15/42 ,  B01J20/04 ,  B01J20/281

CPC classification number: C07K1/14 ,  B01D15/3847 ,  B01D15/424 ,  B01J20/048 ,  B01J20/281 ,  B01J20/282 ,  C07K16/00

Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution.

公开(公告)号：EP2606059A4

公开(公告)日：2015-01-14

申请号：EP11818724

申请日：2011-08-17

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J

IPC: C07K1/00 ,  C07K1/20

CPC classification number: C07K1/18 ,  B01D15/361 ,  B01D15/3804 ,  B01D15/426 ,  C07K1/20 ,  C07K1/22

公开(公告)号：EP2670704A4

公开(公告)日：2014-08-20

申请号：EP12742721

申请日：2012-02-01

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J

IPC: B01D15/20 ,  B08B3/08

CPC classification number: B01D15/203 ,  B01J20/3475 ,  B08B3/08

Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example after a flow-through collection of a target and prior to cleaning the chromatography solid support.