公开(公告)号：CA2766093C

公开(公告)日：2013-12-17

申请号：CA2766093

申请日：2010-06-29

Applicant: BIO RAD LABORATORIES

Inventor： SNYDER MARK A

IPC: B01D15/22 ,  B01D15/08

Abstract: In an axial-flow cylindrical preparative chromatography column that utilizes a piston to close off the top of the resin space inside the column and thereby eliminate void spaces at the top of the resin, a sight glass is incorporated in the column wall to allow monitoring of the packing height and density as the packing material is being placed inside the column, as well as the position of the piston head relative to the packing while the piston is being lowered to consolidated and/or compress the packing. By observing the column interior through the sight glass, the operator can assure that the column receives a more uniform packing density and can minimize the risk of damage to the packing material by excessive force from the piston head.

公开(公告)号：CA2763309C

公开(公告)日：2014-04-01

申请号：CA2763309

申请日：2010-05-18

Applicant: BIO RAD LABORATORIES

Inventor： SNYDER MARK A

IPC: B01D15/22 ,  C07K1/22 ,  C07K17/00 ,  G01N1/34

Abstract: In an affinity-type purification, ligands dissociated from a packed bed that would otherwise leach into the solution containing the species being purified are captured by a second ligand that resides in a porous barrier downstream from the packed bed, the second ligand ex-hibiting an affinity-type interaction with the dissociated first ligand with sufficient specificity to avoid the undesired retention by the second ligand of species from the liquid sample or source liquid other than the species sought to be purified in the affinity column.

公开(公告)号：CA2787009A1

公开(公告)日：2011-07-21

申请号：CA2787009

申请日：2011-01-13

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J ,  SNYDER MARK A

IPC: G01N30/02 ,  G01N33/544

Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution. Specifically, provided are methods for purifying a target molecule in a sample, comprising contacting a sample comprising the target molecule to an apatite solid surface, contacting the solid surface comprising the adsorbed target molecule with a solution comprising a basic amino compound and alkali metal ion or alkali earth ion; or a sulphonated amine compound and alkali metal ion or alkali earth ion, and eluting the target molecule from the solid support by contacting the solid support with a different solution.

公开(公告)号：WO2015200299A3

公开(公告)日：2016-02-18

申请号：PCT/US2015037145

申请日：2015-06-23

Applicant: BIO RAD LABORATORIES

Inventor： SNYDER MARK A ,  BRISACK KIMBERLY C

IPC: B08B3/00

CPC classification number: B01D15/203 ,  B01D15/361 ,  B01D15/3847 ,  B01J20/048 ,  B01J20/3085 ,  B01J20/34 ,  C01B25/32

Abstract: Disclosed herein are methods of regenerating apatite surfaces, for example after purification of a target analyte. In one embodiment (a) comprises binding the target analyte to the apatite solid surface and (b) comprises eluting the target analyte from the apatite surface

Abstract translation: 本文公开的是再生磷灰石表面的方法，例如在纯化目标分析物之后。 在一个实施方案中（a）包括将目标分析物结合到磷灰石固体表面，和（b）包括从磷灰石表面洗脱目标分析物

公开(公告)号：CA2713093A1

公开(公告)日：2009-07-16

申请号：CA2713093

申请日：2009-01-06

Applicant: BIO RAD LABORATORIES

Inventor： SNYDER MARK A ,  BAKER KEN ,  MORAN CHRISTOPHER

IPC: F15B15/28 ,  B01D53/02 ,  G01D5/02

Abstract: In an axial-flow cylindrical preparative chromatography column that utilizes a piston to close off the top of the resin space inside the column and thereby eliminate void spaces at the top of the resin, one or more proximity detectors are incorporated in the piston head to either generate a signal indicating the proximity of the piston had to the resin, or to collect a signal, such as a optical signal, emitted from the resin. The value of the signal or any change in the signal is compared to a threshold value to ascertain when the piston head is in proximity to the resin so that the motion of the piston toward the resin can be halted before contact pressure from the piston head damages the resin.

公开(公告)号：CA2766093A1

公开(公告)日：2011-01-06

申请号：CA2766093

申请日：2010-06-29

Applicant: BIO RAD LABORATORIES

Inventor： SNYDER MARK A

IPC: B01D15/08

Abstract: In an axial-flow cylindrical preparative chromatography column that utilizes a piston to close off the top of the resin space inside the column and thereby eliminate void spaces at the top of the resin, a sight glass is incorporated in the column wall to allow monitoring of the packing height and density as the packing material is being placed inside the column, as well as the position of the piston head relative to the packing while the piston is being lowered to consolidated and/or compress the packing. By observing the column interior through the sight glass, the operator can assure that the column receives a more uniform packing density and can minimize the risk of damage to the packing material by excessive force from the piston head.

公开(公告)号：CA2765006A1

公开(公告)日：2010-12-23

申请号：CA2765006

申请日：2010-06-14

Applicant: BIO RAD LABORATORIES

Inventor： SNYDER MARK A

IPC: B01D15/20

Abstract: ABSTRACT OF THE DISCLOSURE In an axial-flow preparative chromatography column that contains a piston, commonly termed an adaptor, that is lowered over the top of the resin, the ability to lower the piston in a controlled manner to minimize the chances of damage to the resin is achieved by use of an aperture either in the piston head itself or in a compound bolt that secures the upper column frit to the piston head. The aperture is closed with a removable plug during storage and use of the piston head and column. During packing of the bed, however, the plug is replaced by a graduated rod operating as a dipstick to allow the operator to determine the distance between the bottom face of the piston head and the upper surface of the column packing.

公开(公告)号：WO2008067271A2

公开(公告)日：2008-06-05

申请号：PCT/US2007085563

申请日：2007-11-27

Applicant: BIO RAD LABORATORIES ,  SNYDER MARK A

Inventor： SNYDER MARK A

IPC: C07K17/00 ,  G01N33/543

CPC classification number: C07K17/04 ,  B01D15/3804 ,  B01J20/286 ,  B01J20/3229 ,  B01J20/3242 ,  G01N33/54306

Abstract: In an affinity-type purification, ligands dissociated from the stationary phase that would otherwise leach into the species being purified are captured by a second ligand that is also incorporated into the stationary phase, the second ligand exhibiting an affinity-type interaction with the dissociated first ligand with sufficient specificity to avoid the undesired retention by the second ligand of species from the liquid sample or source liquid other than the species sought to be purified in the affinity column.

Abstract translation: 在亲和型纯化中，从固定相解离的否则将浸出到待纯化物质中的配体被还被并入固定相的第二配体捕获，第二配体与解离的第一配体呈亲和型相互作用 配体，其具有足够的特异性以避免第二配体物质从液体样品或源液体中除了在亲和柱中被纯化的物质以外的不期望的保留。

公开(公告)号：CA2787009C

公开(公告)日：2018-04-03

申请号：CA2787009

申请日：2011-01-13

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J ,  SNYDER MARK A

IPC: B01D15/08

Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution. Specifically, provided are methods for purifying a target molecule in a sample, comprising contacting a sample comprising the target molecule to an apatite solid surface, contacting the solid surface comprising the adsorbed target molecule with a solution comprising a basic amino compound and alkali metal ion or alkali earth ion; or a sulphonated amine compound and alkali metal ion or alkali earth ion, and eluting the target molecule from the solid support by contacting the solid support with a different solution.

公开(公告)号：CA2763309A1

公开(公告)日：2010-12-02

申请号：CA2763309

申请日：2010-05-18

Applicant: BIO RAD LABORATORIES

Inventor： SNYDER MARK A

IPC: A23J1/00

Abstract: In an affinity-type purification, ligands dissociated from a packed bed that would otherwise leach into the solution containing the species being purified are captured by a second ligand that resides in a porous barrier downstream from the packed bed, the second ligand exhibiting an affinity-type interaction with the dissociated first ligand with sufficient specificity to avoid the undesired retention by the second ligand of species from the liquid sample or source liquid other than the species sought to be purified in the affinity column.