公开(公告)号：EP2062079A4

公开(公告)日：2010-03-10

申请号：EP07813831

申请日：2007-08-07

Applicant: BIO RAD LABORATORIES

Inventor： HILLENDAHL JAMES W ,  WALDBESER DAVID E

IPC: G02B6/32 ,  G01N21/25 ,  G01N21/64 ,  G02B21/00

CPC classification number: G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2021/6484

公开(公告)号：EP2820676A4

公开(公告)日：2015-10-21

申请号：EP13755695

申请日：2013-01-14

Applicant: BIO RAD LABORATORIES

Inventor： HILLENDAHL JAMES W ,  PEREZ EVELIO

IPC: G01N21/25 ,  F21K99/00 ,  G01N21/64 ,  G01N27/447 ,  H01L33/08

CPC classification number: F21V21/005 ,  F21V9/083 ,  F21V13/02 ,  G01N21/255 ,  G01N21/6452 ,  G01N21/6456 ,  G01N27/447 ,  G01N2021/6463 ,  G01N2021/6471 ,  G01N2201/06 ,  G01N2201/0626 ,  G01N2201/0635

Abstract: A planar sample, particularly of the type used in biological laboratories for detection and sometimes analysis of two-dimensional arrays of proteins, nucleic acids, or other biological species, is illuminated by epi-illumination using optically filtered line lights that are arranged along opposing parallel sides of a rectangle in which the sample array resides, with two coaxial line lights on each side of the rectangle, and the two on any given side being separated by a gap whose optimal width depends on the wavelength band transmitted by the optical filter. Surprisingly, the gap eliminates the peak in intensity at the center of the sample area and the decrease that occurs from the center outward that would otherwise occur with a single continuous filtered line light, producing instead a substantially uniform intensity along the direction parallel to the line lights.

公开(公告)号：EP1438615A4

公开(公告)日：2006-01-25

申请号：EP02773551

申请日：2002-09-24

Applicant: BIO RAD LABORATORIES

Inventor： NAGHIEH HARRY R ,  HILLENDAHL JAMES W ,  WALDBESER DAVID E

IPC: G01N21/25 ,  G01N21/64 ,  H04N1/04 ,  G02B6/26

CPC classification number: G01N21/6452 ,  G01N2021/6484 ,  Y10T436/11

Abstract: Microarrays (11) are imaged by an illumination and detection system that supplies excitation light through one or more optical fibers (20), each fiber transmitting excitation light from an excitation light source (14) to a single spot (12) in the microarray (11). Emission light from each spot is then collected by a collimating lens (15) and converted to a signal that is compiled by conventional software into an image of the entire microarray (11). The optical fiber(s) (20) and the collimating lens (15) are arranged such that the direction of travel of the excitation light (14) and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the plane of the microarray.

公开(公告)号：EP1430341A4

公开(公告)日：2006-01-18

申请号：EP02763709

申请日：2002-09-24

Applicant: BIO RAD LABORATORIES

Inventor： HILLENDAHL JAMES W ,  WALDBESER DAVID E ,  NAGHIEH HARRY R

IPC: G01N21/25 ,  G01N21/64 ,  G02B6/32

CPC classification number: G01N21/253 ,  G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2021/6484

Abstract: Biochemical assays that are performed in multi-well plates (11) and that utilize a system that supplies excitation light through an optical fiber (21, 22) that transmits light from an excitation light source (14) to the well (12). Emission light produced by the excitation is then collected by a collimating lens (31) and converted to a signal that is compiled for analysis. The optical fiber (21, 22) and collimating lens (31) can either be on the same side of the receptacle or on opposite sides, i.e., one above the other below. When the optical fiber and the collimating lens are both on the open side of the receptacle, they are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the mouth of the receptacle.