公开(公告)号：EP2986359A4

公开(公告)日：2017-02-15

申请号：EP15761295

申请日：2015-03-11

Applicant: BIO-RAD LABORATORIES INC

Inventor： STRONG WILLIAM

IPC: G01N27/447 ,  B01D57/02 ,  C02F1/40 ,  G01N30/90

CPC classification number: G01N27/44739 ,  G01N30/90 ,  G01N33/54386

公开(公告)号：EP2986968A4

公开(公告)日：2017-03-22

申请号：EP15758608

申请日：2015-03-09

Applicant: BIO-RAD LABORATORIES INC

Inventor： MCKEE CLAYTON T ,  STRONG WILLIAM

IPC: G01N21/00 ,  G01N21/75 ,  G01N33/559

CPC classification number: B01L3/502738 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2300/045 ,  B01L2300/047 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/065 ,  C12Q1/6806 ,  G01N27/44717 ,  G01N27/44739 ,  G01N33/54366

Abstract: Devices, systems, methods, and kits are provided for performing separation, immobilization, blotting, and/or detection of analytes from biological samples. In some embodiments, the devices are constructed from two solid substrates with surfaces in contact. The devices include a plurality of channels formed from indentations in these surfaces. The indentations can be aligned with each other across the interface between the substrates, and realigned by shifting or sliding one substrate relative to the other. In some embodiments, the devices are constructed from three layers of a solid substrate. A separation channel in the middle layer of the device is first used for analyte separation. The middle layer can then be slid relative the top and/or bottom layer, thereby aligning the separation channel with a blotting membrane. Analytes can then be transferred to the membrane using electrodes in the top and bottom layers.

公开(公告)号：EP2986646A4

公开(公告)日：2017-04-12

申请号：EP14834424

申请日：2014-08-08

Applicant: BIO-RAD LABORATORIES INC

Inventor： MCKEE CLAYTON T ,  STRONG WILLIAM ,  OLECH LEE ,  BERKELMAN THOMAS ,  BELISLE CHRISTOPHER

IPC: G01N33/58 ,  G01N27/447 ,  G01N33/68

CPC classification number: G01N33/5306 ,  G01N27/44747 ,  G01N33/582 ,  G01N33/6803 ,  G01N2400/18

Abstract: A method is provided for detecting a protein using a cyclodextrin covalently linked to at least one label. The cyclodextrin can associate with the protein by sequestering an aromatic amino acid side-chain of the protein in its hydrophobic cavity. After contacting the protein with the cyclodextrin, the label can be detected directly or can undergo a chemical interaction with a reagent to form a detectable product. The label can include an indole moiety, which can react with a halo-substituted organic compound upon exposure to UV light and thereby be rendered fluorescent. Alternatively, the label can include a biotin moiety, which can bind to a binding partner such as avidin, or variants thereof, to form a detectable molecular complex. A labeled cyclodextrin can be used in the present methods to detect a protein of interest in an electrophoresis gel or on a blotting membrane. Aromatic amino acid residues of the protein, in particular tryptophan, remain protected from chemical modification due to sequestration by the cyclodextrin, making these methods compatible with downstream applications that require intact protein. Also provided herein are compositions, kits, and electrophoresis gels for use in detecting proteins.

公开(公告)号：EP2509681A4

公开(公告)日：2014-09-03

申请号：EP10836707

申请日：2010-12-09

Applicant: BIO RAD LABORATORIES

Inventor： LATHAM MATTHEW ,  STRONG WILLIAM ,  CHMIEL NIKOLAS ,  XU JEFF ,  IOVANNI ROBERT

IPC: G01N27/447 ,  A44B17/00 ,  A61N1/30 ,  B01D57/02

CPC classification number: G01N27/44739 ,  B01D57/02 ,  G01N27/44782

Abstract: Electrotransfer is performed in an instrument that receives electroblotting cassettes and that contains an integrated power supply, controls, and a display that allows the user to monitor and control each of a plurality of cassettes individually through electrical contacts within the housing that mate with corresponding electrical contacts on the cassettes.

Abstract translation: 电子转印在接收电印盒的仪器中进行，并且包含集成的电源，控制和显示器，其允许用户通过壳体内的电接触单独地监测和控制多个盒中的每一个，从而与相应的电触点 在盒子上

公开(公告)号：EP3024843A4

公开(公告)日：2017-03-29

申请号：EP14830330

申请日：2014-07-24

Applicant: BIO-RAD LABORATORIES INC

Inventor： MCKEE CLAYTON T ,  STRONG WILLIAM

IPC: C07K1/16 ,  A61K38/02 ,  C07K1/18

CPC classification number: C07K1/1136 ,  C07K1/22

Abstract: Compositions, methods, articles of manufacture, and kits are provided for storage and delivery of proteins.

Abstract translation: 组合物，方法，制造的物品和试剂盒提供了用于存储和蛋白质的交付。

公开(公告)号：EP2986978A4

公开(公告)日：2016-11-30

申请号：EP14867041

申请日：2014-12-02

Applicant: BIO RAD LABORATORIES

Inventor： STRONG WILLIAM

IPC: G01N27/447

CPC classification number: G01N27/44704

Abstract: Molds for casting and retaining electrophoresis gel strips are provided, along with methods, kits, and systems for performing electrophoresis, electroelution, and/or electroblotting using these molds.

公开(公告)号：EP1664720A4

公开(公告)日：2008-01-16

申请号：EP04783740

申请日：2004-09-10

Applicant: BIO RAD LABORATORIES

Inventor： PROVOST ROGER ,  STRONG WILLIAM

IPC: G01N27/447 ,  G01N27/26 ,  G01N27/453

CPC classification number: G01N27/44717 ,  G01N27/44704

Abstract: Gel plugs that can be stored in a sealed condition and delivered individually to an electrophoresis gel as standards for electrophoretic separations are retained in a cartridge that is cleanly breakable into individual sections, each section retaining a gel plug of predefined dimensions with standard solute mixtures embedded in the plug. The separation of any single section from the cartridge exposes opposing edges of the gel plug which permits the user to insert an implement into the section interior and to urge the plug out of the section and into a well of an electrophoresis gel. All manipulations are performed without any need for contact between the user and the gel.