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    11.
    发明专利
    未知

    公开(公告)号：DE60114217D1

    公开(公告)日：2005-11-24

    申请号：DE60114217

    申请日：2001-08-03

    Applicant: BIO RAD LABORATORIES

    Inventor： ZHU MINGDE ,  OLECH LEE

    IPC: G01N33/58 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543 ,  G01N33/68

    Abstract: The proteins in a biological sample that is sought to be analyzed for its protein composition by an electrophoretic or chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a solid dry composition containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The solid and dry form of the composition prevents the dye from deteriorating or decomposing, and the combination of components in the composition allows the dye to couple to the proteins in a relatively uniform manner with no overstaining of the protein when the composition and the sample are heated together and held at an elevated temperature for a short period of time.

    Encapsulated IPG strips
    12.
    发明专利
    Encapsulated IPG strips 未知

    公开(公告)号：AU1127900A

    公开(公告)日：2000-06-13

    申请号：AU1127900

    申请日：1999-10-20

    Applicant: BIO RAD LABORATORIES

    Inventor： OLECH LEE ,  HARBERS ADRIANA J

    IPC: G01N27/447 ,  B01D57/02

    Abstract: Immobilized pH gradient (IPG) strips are encapsulated in an enclosure that seals the strip from atmospheric exposure and yet contains sufficient volumetric capacity to accommodate the strip in rehydrated form. The enclosure contains a pair of electrode access apertures each of which is spanned by a dialysis membrane to permit electrical contact between external electrodes and the encapsulated IPG strip, and one or more sample application apertures through which liquid sample can be applied to the strip. Each of the apertures is covered by a protective cover or flap that is removable to expose the aperture.

    ENCAPSULATED IPG STRIPS
    13.
    发明专利
    ENCAPSULATED IPG STRIPS 未知

    公开(公告)号：CA2318542A1

    公开(公告)日：2000-06-02

    申请号：CA2318542

    申请日：1999-10-20

    Applicant: BIO RAD LABORATORIES

    Inventor： OLECH LEE ,  HARBERS ADRIANA J

    IPC: G01N27/447 ,  B01D57/02

    Abstract: Immobilized pH gradient (IPG) strips are encapsulated in an enclosure that seals the strip (12) from atmospheric exposure and yet contains sufficient volumetric capacity (15) to accommodate the strip in rehydrated form. The enclosure contains a pair of electrode access apertures (21, 22) each of which is spanned by a dialysis membrane to permit electrical contact between external electrodes and the encapsulated IPG strip, and one or more sample application apertures (23) through which lipid sample can be applied to the strip. Each of the apertures is covered by a protective cover or flap (24, 25, 26) that is removable to expose the aperture.

    14.
    发明专利
    未知

    公开(公告)号：DE69926725T2

    公开(公告)日：2006-06-29

    申请号：DE69926725

    申请日：1999-10-20

    Applicant: BIO RAD LABORATORIES

    Inventor： OLECH LEE ,  HARBERS J

    IPC: G01N27/447 ,  B01D57/02

    Abstract: Immobilized pH gradient (IPG) strips are encapsulated in an enclosure that seals the strip from atmospheric exposure and yet contains sufficient volumetric capacity to accommodate the strip in rehydrated form. The enclosure contains a pair of electrode access apertures each of which is spanned by a dialysis membrane to permit electrical contact between external electrodes and the encapsulated IPG strip, and one or more sample application apertures through which liquid sample can be applied to the strip. Each of the apertures is covered by a protective cover or flap that is removable to expose the aperture.

    Encapsulated IPG strips
    15.
    发明专利
    Encapsulated IPG strips 未知

    公开(公告)号：AU762384B2

    公开(公告)日：2003-06-26

    申请号：AU1127900

    申请日：1999-10-20

    Applicant: BIO RAD LABORATORIES

    Inventor： OLECH LEE ,  HARBERS ADRIANA J

    IPC: G01N27/447 ,  B01D57/02

    Abstract: Immobilized pH gradient (IPG) strips are encapsulated in an enclosure that seals the strip from atmospheric exposure and yet contains sufficient volumetric capacity to accommodate the strip in rehydrated form. The enclosure contains a pair of electrode access apertures each of which is spanned by a dialysis membrane to permit electrical contact between external electrodes and the encapsulated IPG strip, and one or more sample application apertures through which liquid sample can be applied to the strip. Each of the apertures is covered by a protective cover or flap that is removable to expose the aperture.

    Dry powder formulation for protein labeling
    16.
    发明专利
    Dry powder formulation for protein labeling 未知

    公开(公告)号：AU7814101A

    公开(公告)日：2002-03-04

    申请号：AU7814101

    申请日：2001-08-03

    Applicant: BIO RAD LABORATORIES

    Inventor： ZHU MINGDE ,  OLECH LEE

    IPC: G01N33/58 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543 ,  G01N33/68

    Abstract: The proteins in a biological sample that is sought to be analyzed for its protein composition by an electrophoretic or chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a solid dry composition containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The solid and dry form of the composition prevents the dye from deteriorating or decomposing, and the combination of components in the composition allows the dye to couple to the proteins in a relatively uniform manner with no overstaining of the protein when the composition and the sample are heated together and held at an elevated temperature for a short period of time.

    DRY POWDER FORMULATION FOR PROTEIN LABELING
    17.
    发明专利
    DRY POWDER FORMULATION FOR PROTEIN LABELING 未知

    公开(公告)号：CA2420149A1

    公开(公告)日：2002-02-28

    申请号：CA2420149

    申请日：2001-08-03

    Applicant: BIO RAD LABORATORIES

    Inventor： OLECH LEE ,  ZHU MINGDE

    IPC: G01N33/58 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543 ,  G01N33/68

    Abstract: The proteins in a biological sample that is sought to be analyzed for its protein composition by a chromatographic procedure are coupled to a dye in a n unusually efficient manner by combining the sample with a dry powdered mixtu re containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The dry powdered form of the mixture avoids deterioration or decomposition of the dye, and the combination of components in the mixture allows the dye to couple to the proteins in a relatively uniform and highly controllable manner when the powder mixture and sample ar e heated together and held at an elevated temperature until some or all of the powder dissolves.

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