АНАЛИЗ НУКЛЕИНОВЫХ КИСЛОТ, ПОЛУЧЕННЫХ ИЗ ЕДИНИЧНЫХ КЛЕТОК

    公开(公告)号:EA020593B1

    公开(公告)日:2014-12-30

    申请号:EA201170933

    申请日:2010-01-13

    Applicant: FLUIDIGM CORP

    Abstract: Изобретениеотноситсяк способаманализагеномнойДНКи/илиРНКизнебольшихобразцовилидажеединичныхклеток. СпособыанализагеномнойДНКмогутвключатьполногеномнуюамплификацию (WGA) споследующейпреамплификациейи амплификациейвыбранныхнуклеиновыхкислот-мишеней. СпособыанализаРНКмогутвключатьобратнуютранскрипциюжелательнойРНКс последующейпреамплификациейи амплификациейвыбранныхнуклеиновыхкислот-мишеней.

    CRYSTAL FORMING DEVICES AND SYSTEMS AND METHODS FOR MAKING AND USING THE SAME

    公开(公告)号:SG10201506381RA

    公开(公告)日:2015-09-29

    申请号:SG10201506381R

    申请日:2005-01-25

    Applicant: FLUIDIGM CORP

    Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.

    CRYSTAL FORMING DEVICES AND SYSTEMS AND METHODS FOR MAKING AND USING THE SAME

    公开(公告)号:SG10201405756WA

    公开(公告)日:2014-11-27

    申请号:SG10201405756W

    申请日:2005-01-25

    Applicant: FLUIDIGM CORP

    Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.

    CRYSTAL FORMING DEVICES AND SYSTEMS AND METHODS FOR MAKING AND USING THE SAME

    公开(公告)号:SG152261A1

    公开(公告)日:2009-05-29

    申请号:SG2009027566

    申请日:2005-01-25

    Applicant: FLUIDIGM CORP

    Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.

    CRYSTAL FORMING DEVICES AND SYSTEMS AND METHODS FOR MAKING AND USING THE SAME

    公开(公告)号:CA2554240A1

    公开(公告)日:2005-08-11

    申请号:CA2554240

    申请日:2005-01-25

    Applicant: FLUIDIGM CORP

    Abstract: The present invention provides for microfluidic devices and methods for thei r use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generate d from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments , are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen again st the microfluidic device so that at least one of the fluid ports of the plate n is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure i s changed therein.

    Crystal forming devices and systems and methods for making and using the same

    公开(公告)号:AU2010214783A1

    公开(公告)日:2010-09-23

    申请号:AU2010214783

    申请日:2010-09-02

    Applicant: FLUIDIGM CORP

    Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.

    Crystal forming devices and systems and methods for making and using the same

    公开(公告)号:AU2005208879B2

    公开(公告)日:2010-06-03

    申请号:AU2005208879

    申请日:2005-01-25

    Applicant: FLUIDIGM CORP

    Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.

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