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公开(公告)号:EA020593B1
公开(公告)日:2014-12-30
申请号:EA201170933
申请日:2010-01-13
Applicant: FLUIDIGM CORP
Inventor: HAMILTON AMY , LIN MIN , MIR ALAIN , PIEPRZYK MARTIN
Abstract: Изобретениеотноситсяк способаманализагеномнойДНКи/илиРНКизнебольшихобразцовилидажеединичныхклеток. СпособыанализагеномнойДНКмогутвключатьполногеномнуюамплификацию (WGA) споследующейпреамплификациейи амплификациейвыбранныхнуклеиновыхкислот-мишеней. СпособыанализаРНКмогутвключатьобратнуютранскрипциюжелательнойРНКс последующейпреамплификациейи амплификациейвыбранныхнуклеиновыхкислот-мишеней.
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公开(公告)号:SG166024A1
公开(公告)日:2010-11-29
申请号:SG2009028630
申请日:2005-05-02
Applicant: FLUIDIGM CORP
Inventor: GOODSAID FEDERICO , UNGER MARC A , HUANG JIANG , QUAN EMERSON , GROSSMAN ROBERT , LAM PHILLIP , CHOU HOU-PU , KIMBALL JAKE , PIEPRZYK MARTIN , FACER GEOFFREY
Abstract: An M x N matrix microfluidic device for performing a matrix of reactions, the device (100) having a plurality of reaction cells (106) in communication with one of either a sample inlet (120) or a reagent t inlet (124) through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method includes using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block. Fig. 1A
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公开(公告)号:SG10201506381RA
公开(公告)日:2015-09-29
申请号:SG10201506381R
申请日:2005-01-25
Applicant: FLUIDIGM CORP
Inventor: GROSSMAN ROBERT , UNGER MARC , LAM PHILLIP , CHOU HOU-PU , KIMBALL JAKE , PIEPRZYK MARTIN
Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.
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公开(公告)号:SG10201405756WA
公开(公告)日:2014-11-27
申请号:SG10201405756W
申请日:2005-01-25
Applicant: FLUIDIGM CORP
Inventor: GROSSMAN ROBERT , UNGER MARC , LAM PHILLIP , CHOU HOU-PU , KIMBALL JAKE , PIEPRZYK MARTIN
IPC: C12M1/00 , B01J19/00 , B01L3/00 , B01L3/06 , B01L9/00 , B01L99/00 , C12M3/00 , C30B7/00 , C30B29/58
Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.
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公开(公告)号:SG152261A1
公开(公告)日:2009-05-29
申请号:SG2009027566
申请日:2005-01-25
Applicant: FLUIDIGM CORP
Inventor: GROSSMAN ROBERT , UNGER MARC , LAM PHILLIP , CHOU HOU-PU , KIMBALL JAKE , PIEPRZYK MARTIN
Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.
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公开(公告)号:CA2554240A1
公开(公告)日:2005-08-11
申请号:CA2554240
申请日:2005-01-25
Applicant: FLUIDIGM CORP
Inventor: PIEPRZYK MARTIN , KIMBALL JAKE , CHOU HOU-PU , UNGER MARC , LAM PHILLIP , GROSSMAN ROBERT
IPC: G01N1/28 , B01J19/00 , B01L3/00 , B01L3/06 , B01L9/00 , B01L99/00 , B81B1/00 , B81B7/00 , C30B7/00 , C30B29/58 , G01N35/02 , G01N37/00
Abstract: The present invention provides for microfluidic devices and methods for thei r use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generate d from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments , are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen again st the microfluidic device so that at least one of the fluid ports of the plate n is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure i s changed therein.
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公开(公告)号:EP2379753A4
公开(公告)日:2012-05-30
申请号:EP10732056
申请日:2010-01-13
Applicant: FLUIDIGM CORP
Inventor: HAMILTON AMY , LIN MIN , MIR ALAIN , PIEPRZYK MARTIN
CPC classification number: C12Q1/6881 , C12Q1/6844 , C12Q1/686 , C12Q2531/113 , C12Q2531/119 , C12Q2531/137 , C12Q2565/101 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , C12Q2600/178
Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.
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公开(公告)号:SG2013006333A
公开(公告)日:2014-08-28
申请号:SG2013006333
申请日:2005-05-02
Applicant: FLUIDIGM CORP
Inventor: GOODSAID FEDERICO , UNGER MARC A , HUANG JIANG , QUAN EMERSON , GROSSMAN ROBERT , LAM PHILLIP , CHOU HOU-PU , KIMBALL JAKE , PIEPRZYK MARTIN , FACER GEOFFREY
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公开(公告)号:AU2010214783A1
公开(公告)日:2010-09-23
申请号:AU2010214783
申请日:2010-09-02
Applicant: FLUIDIGM CORP
Inventor: GROSSMAN ROBERT , UNGER MARC , LAM PHILLIP , CHOU HOU-PU , KIMBALL JAKE , PIEPRZYK MARTIN
IPC: G01N15/06 , B01J19/00 , B01L3/00 , B01L3/06 , B01L9/00 , B01L99/00 , C30B7/00 , C30B29/58 , G01N33/00 , G01N33/48
Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.
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公开(公告)号:AU2005208879B2
公开(公告)日:2010-06-03
申请号:AU2005208879
申请日:2005-01-25
Applicant: FLUIDIGM CORP
Inventor: CHOU HOU-PU , GROSSMAN ROBERT , LAM PHILLIP , KIMBALL JAKE , PIEPRZYK MARTIN , UNGER MARC
IPC: G01N15/06 , B01J19/00 , B01L3/00 , B01L3/06 , B01L9/00 , B01L99/00 , C30B7/00 , C30B29/58 , G01N33/00 , G01N33/48
Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.
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