公开(公告)号：CA2915475A1

公开(公告)日：2014-11-27

申请号：CA2915475

申请日：2014-05-27

Applicant: LIFE TECHNOLOGIES CORP

Inventor： PALLAS MICHAEL ,  FOSTER EVAN ,  FONSECA JORGE ,  NURSE JAMES ,  STRAUB THEODORE

IPC: B01L3/00

Abstract: A system for processing a plurality of biological samples contains a support and a temperature controller. The support is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. The temperature controller is configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples. The support is also configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction.

公开(公告)号：SG11201510642UA

公开(公告)日：2016-02-26

申请号：SG11201510642U

申请日：2014-05-27

Applicant: LIFE TECHNOLOGIES CORP

Inventor： PALLAS MICHAEL ,  FOSTER EVAN ,  FONSECA JORGE ,  NURSE JAMES ,  STRAUB THEODORE

IPC: B01L3/00

Abstract: A system for processing a plurality of biological samples contains a support and a temperature controller. The suppose is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. The temperature controller is configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples. The support is also configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction.

公开(公告)号：SG11201405787SA

公开(公告)日：2014-11-27

申请号：SG11201405787S

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORP

Inventor： PALLAS MICHAEL C ,  NURSE JAMES ,  LIM GARY ,  STRAUB THEODORE ,  FOSTER EVAN

IPC: B01L3/02 ,  G01N35/10

Abstract: An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.

公开(公告)号：SG11201405785WA

公开(公告)日：2014-11-27

申请号：SG11201405785W

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORP

Inventor： PALLAS MICHAEL C ,  NURSE JAMES ,  LIM GARY ,  STRAUB THEODORE ,  GHENCIU ELIODOR ,  FOSTER EVAN ,  FONSECA JORGE ,  MAHER KEVIN

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/68

Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

公开(公告)号：AU2013231955A1

公开(公告)日：2014-10-30

申请号：AU2013231955

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORP

Inventor： PALLAS MICHAEL C ,  NURSE JAMES ,  LIM GARY ,  STRAUB THEODORE ,  FOSTER EVAN

IPC: B01L3/02

Abstract: A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. The sample loader further comprises a flow path between the first blade and second blade configured to dispense a liquid sample to a substrate including a plurality of reaction sites. Further, in various embodiments the liquid sample has an advancing contact angle of 85 +/- 15 degrees with the first and second blade. Furthermore, loading of the liquid sample dispensed from the flow path to the plurality of reaction sites may be based on capillary action.