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    POLYNUCLEOTIDE ADAPTERS AND METHODS OF USE THEREOF
    11.
    发明申请
    POLYNUCLEOTIDE ADAPTERS AND METHODS OF USE THEREOF 审中-公开

    公开(公告)号：WO2018175798A1

    公开(公告)日：2018-09-27

    申请号：PCT/US2018/023872

    申请日：2018-03-22

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： ANDERSEN, Mark ,  ALLEN, Michael

    IPC: C12Q1/6855

    Abstract: Provided are methods and compositions for reducing unfavorable dimer formation and thereby improving library preparation, e.g., for sequencing. Compositions and methods include adapters comprising extensive 5' overhang sequences and blunt end or T overhang 3' sequences to ends of target nucleic acid(s) to facilitate amplification and analysis of such sequences.

    MULTIPLEX TRANSCRIPTOME ANALYSIS
    13.
    发明申请
    MULTIPLEX TRANSCRIPTOME ANALYSIS 审中-公开
    多重代码分析

    公开(公告)号：WO2016025796A1

    公开(公告)日：2016-02-18

    申请号：PCT/US2015/045209

    申请日：2015-08-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： BRAMLETT, Kelli ,  LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

    IPC: C12Q1/68

    CPC classification number: C12Q1/6846 ,  C12Q1/6855 ,  C12Q2525/117 ,  C12Q2535/10 ,  C12Q2537/143 ,  C12Q2525/191 ,  C12Q2563/185

    Abstract: In some embodiments, the disclosure relates generally to methods, compositions, systems, apparatuses and kits comprising a multiplex nucleic acid amplification reaction that employs a plurality (e.g., hundreds, thousands, tens-of-thousands or hundreds-of-thousands) of different target-specific primer pairs that enable substantially simultaneous amplification of a plurality of different target sequences-of-interest in a single reaction mixture. In some embodiments, the multiplex nucleic acid amplification reaction generates a plurality of amplicons having sequences derived from a sample containing RNA or DNA, including whole transcriptome or genomic samples. In some embodiments, the sequences and abundances of at least some of the plurality of amplicons are characterized, optionally simultaneously or through a single assay, by suitable detection methods, including sequencing or other procedures known in the art.

    Abstract translation: 在一些实施方案中，本公开一般涉及包含多重核酸扩增反应的方法，组合物，系统，装置和试剂盒，所述多重核酸扩增反应采用多个（例如，数百，数万，数万或数十万）不同的 靶特异性引物对，其能够在单个反应混合物中基本上同时扩增感兴趣的多个不同靶序列。 在一些实施方案中，多重核酸扩增反应产生具有衍生自含有RNA或DNA的样品的序列的多个扩增子，包括全转录组或基因组样品。 在一些实施方案中，通过合适的检测方法，包括测序或本领域已知的其它程序，可以任选地同时或通过单个测定来表征多个扩增子中的至少一些的序列和丰度。

    COMPOSITIONS, METHODS, SYSTEMS AND KITS FOR TARGET NUCLEIC ACID ENRICHMENT
    17.
    发明授权
    COMPOSITIONS, METHODS, SYSTEMS AND KITS FOR TARGET NUCLEIC ACID ENRICHMENT 有权
    目标核酸富集的组合物，方法，系统和试剂盒

    公开(公告)号：EP2906715B1

    公开(公告)日：2017-07-26

    申请号：EP13783775.3

    申请日：2013-10-15

    Applicant: Life Technologies Corporation

    Inventor： ANDERSEN, Mark ,  ROMAN, Steven

    IPC: C12Q1/68 ,  C12N15/10

    CPC classification number: C12Q1/6806 ,  C07H21/00 ,  C12Q1/6853 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2563/131 ,  C12Q2563/149

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating one or more target nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the amount of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture using a primer having a first primer sequence and a second primer sequence that are complementary at a first melting temperature and are not complementary at a second melting temperature. In some aspects, target nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and template library preparation.

    Abstract translation: 本发明提供了用于从样品中分离核酸分子的方法，组合物，试剂盒，系统和设备。 特别地，所述方法通常涉及标准化来自样品的靶核酸分子的浓度。 一方面，本发明涉及从引物延伸反应混合物中纯化引物延伸产物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的核酸分子可以用于各种下游过程，包括核酸测序。

    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    19.
    发明公开
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 审中-公开

    公开(公告)号：EP3495497A1

    公开(公告)日：2019-06-12

    申请号：EP18194509.8

    申请日：2012-04-27

    Applicant: Life Technologies Corporation

    Inventor： LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

    IPC: C12Q1/68

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

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