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    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    1.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 审中-公开

    公开(公告)号：WO2012149438A9

    公开(公告)日：2012-11-01

    申请号：PCT/US2012/035612

    申请日：2012-04-27

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

    Inventor： LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

    IPC: C12Q1/68

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    2.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 审中-公开
    用于多重PCR的方法和组合物

    公开(公告)号：WO2013081864A1

    公开(公告)日：2013-06-06

    申请号：PCT/US2012/065650

    申请日：2012-11-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

    IPC: C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2537/16

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

    Abstract translation: 本发明提供可用于样品中存在的一种或多种核酸的多重PCR的方法，组合物，试剂盒，系统和装置。 具体而言，提供了允许选择性扩增一个或多个靶序列的各种靶特异性引物。 一方面，本发明涉及用于选择性扩增与癌症或遗传疾病相关的一个或多个靶序列的靶特异性引物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的扩增的靶序列可用于各种下游过程，包括核酸测序并用于检测遗传变体的存在。

    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    3.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 审中-公开
    多重PCR的方法和组合

    公开(公告)号：WO2013081755A1

    公开(公告)日：2013-06-06

    申请号：PCT/US2012/062494

    申请日：2012-10-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

    IPC: C12Q1/68

    CPC classification number: C12Q1/6858 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2537/16

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for determining copy number variation of one or more nucleic acids present in a sample. In some aspects, the method includes various target-specific primers that allow for the selective amplification of one or more target nucleic acids in the sample. In yet another aspect, the invention relates to determining copy number variation with respect to gene or chromosome representation of a nucleic acid in the sample. In some aspects, the method for determining copy number variation of different target nucleic acids in a sample using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including diagnosis, predictive therapeutic regimes or other therapeutic purposes.

    Abstract translation: 本发明提供了可用于确定样品中存在的一种或多种核酸的拷贝数变异的方法，组合物，试剂盒，系统和装置。 在一些方面，该方法包括允许选择性扩增样品中一种或多种靶核酸的各种靶特异性引物。 在另一方面，本发明涉及确定相对于样品中核酸的基因或染色体表示的拷贝数变异。 在一些方面，使用所公开的方法，试剂盒，系统和装置确定样品中不同靶核酸的拷贝数变异的方法可以用于包括诊断，预测性治疗方案或其他治疗目的的各种下游过程。

    MULTIPLEX TRANSCRIPTOME ANALYSIS
    4.
    发明申请
    MULTIPLEX TRANSCRIPTOME ANALYSIS 审中-公开
    多重代码分析

    公开(公告)号：WO2016025796A1

    公开(公告)日：2016-02-18

    申请号：PCT/US2015/045209

    申请日：2015-08-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： BRAMLETT, Kelli ,  LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

    IPC: C12Q1/68

    CPC classification number: C12Q1/6846 ,  C12Q1/6855 ,  C12Q2525/117 ,  C12Q2535/10 ,  C12Q2537/143 ,  C12Q2525/191 ,  C12Q2563/185

    Abstract: In some embodiments, the disclosure relates generally to methods, compositions, systems, apparatuses and kits comprising a multiplex nucleic acid amplification reaction that employs a plurality (e.g., hundreds, thousands, tens-of-thousands or hundreds-of-thousands) of different target-specific primer pairs that enable substantially simultaneous amplification of a plurality of different target sequences-of-interest in a single reaction mixture. In some embodiments, the multiplex nucleic acid amplification reaction generates a plurality of amplicons having sequences derived from a sample containing RNA or DNA, including whole transcriptome or genomic samples. In some embodiments, the sequences and abundances of at least some of the plurality of amplicons are characterized, optionally simultaneously or through a single assay, by suitable detection methods, including sequencing or other procedures known in the art.

    Abstract translation: 在一些实施方案中，本公开一般涉及包含多重核酸扩增反应的方法，组合物，系统，装置和试剂盒，所述多重核酸扩增反应采用多个（例如，数百，数万，数万或数十万）不同的 靶特异性引物对，其能够在单个反应混合物中基本上同时扩增感兴趣的多个不同靶序列。 在一些实施方案中，多重核酸扩增反应产生具有衍生自含有RNA或DNA的样品的序列的多个扩增子，包括全转录组或基因组样品。 在一些实施方案中，通过合适的检测方法，包括测序或本领域已知的其它程序，可以任选地同时或通过单个测定来表征多个扩增子中的至少一些的序列和丰度。

    MODULES AND CALIBRATION METHOD FOR A ROBOTIC POLYNUCLEOTIDE SAMPLE PREPARATION SYSTEM
    5.
    发明申请
    MODULES AND CALIBRATION METHOD FOR A ROBOTIC POLYNUCLEOTIDE SAMPLE PREPARATION SYSTEM 审中-公开
    机器人聚合物样品制备系统的模块和校准方法

    公开(公告)号：WO2013165594A1

    公开(公告)日：2013-11-07

    申请号：PCT/US2013/031640

    申请日：2013-03-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： BARBEE, Kristopher ,  JONES, Ryan ,  MCCUSKER, Sean ,  CARPINO, Maximilian ,  LEAMON, John ,  SCHULTZ, Jonathan ,  HOSHIZAKI, Jon

    IPC: B01L7/00 ,  C12Q1/68 ,  G01N35/10 ,  G01N35/00

    CPC classification number: C12Q1/6806 ,  B04B5/0421 ,  B04B13/00 ,  C12Q1/6869 ,  G01N35/00693 ,  G01N35/1011 ,  G01N2035/00495 ,  G01N2035/1013 ,  G01N2035/103

    Abstract: An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a centrifuge for breaking the emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm. A method for calibrating the z-position of a translation device moving a pipette Hp is included. An imaging system is used for assessing attachement of pipette tip.

    Abstract translation: 一种装置包括机器人系统，该机器人系统在三个正交方向上提供可操作以接收移液管尖端的臂并促进流体进入和移出移液管尖端的运动。 此外，该装置可以包括用于接收移液管吸头的托盘，用于接收管的容器，用于形成乳液的装置，用于破乳的离心机，用于形成包括多核苷酸的拷贝的颗粒的装置，用于富集 颗粒和用于将这种颗粒装载到传感器阵列上的装置。 该装置还可以包括容纳试剂溶液容器的容器。 可选地，除了移液管接收臂之外，机器人还可以包括夹持臂。 包括用于校准移动移液管Hp的翻译装置的z位置的方法。 成像系统用于评估移液管尖端的附着。

    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    7.
    发明公开
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 有权
    方法和组合物多重PCR

    公开(公告)号：EP2785860A1

    公开(公告)日：2014-10-08

    申请号：EP12781556.1

    申请日：2012-10-29

    Applicant: Life Technologies Corporation

    Inventor： LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

    IPC: C12Q1/68

    CPC classification number: C12Q1/6858 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2537/16

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for determining copy number variation of one or more nucleic acids present in a sample. In some aspects, the method includes various target-specific primers that allow for the selective amplification of one or more target nucleic acids in the sample. In yet another aspect, the invention relates to determining copy number variation with respect to gene or chromosome representation of a nucleic acid in the sample. In some aspects, the method for determining copy number variation of different target nucleic acids in a sample using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including diagnosis, predictive therapeutic regimes or other therapeutic purposes.

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