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公开(公告)号：WO2022159874A1
公开(公告)日：2022-07-28
申请号：PCT/US2022/013665
申请日：2022-01-25
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： BRZOSKA, Pius , WILDE, Joyce , FURTADO, Manohar , KENDRICK, Sara , ULEKLEIV, Camilla , STEVENS, Junko , VARMA, Kamini , HAYASHIBARA, Kathleen
IPC: C12Q1/70 , C12Q1/6888
Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of SARS-CoV-2, including SARS-CoV-2 variants, or other coronaviruses from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.
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公开(公告)号：WO2017218938A1
公开(公告)日：2017-12-21
申请号：PCT/US2017/037947
申请日：2017-06-16
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： PAGANI, Ioanna , BRZOSKA, Pius , LI, Kelly , PATEL, Sunali , HUANG, Boli , VARMA, Kamini , PURI, Nitin , DIAMOND, Evan
IPC: C12Q1/68
Abstract: Methods, compositions and kits for detecting microorganisms and/or profiling microbiota such as for example through use of nucleic acid amplification and detection.
Abstract translation: 用于检测微生物和/或分析微生物群的方法，组合物和试剂盒，例如通过使用核酸扩增和检测。
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公开(公告)号：WO2016053881A1
公开(公告)日：2016-04-07
申请号：PCT/US2015/052675
申请日：2015-09-28
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： SCHREIBER, Edgar , VARMA, Kamini , ANDERSEN, Mark
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6806 , C12Q1/6853 , C12Q1/6858 , C12Q1/6869 , C12Q2537/143 , C12Q2537/149 , C12Q2537/159 , C12Q2549/119 , C12Q2535/122 , C12Q2565/125 , C12Q2521/325 , C12Q2525/113 , C12Q2525/125 , C12Q2535/101
Abstract: Methods, compositions and kits are described for resequencing, confirming or verifying Next Generation Sequencing (NGS) results with Sanger Sequencing. These methods are particularly useful for samples having very limited quantities such as formalin-fixed, paraffin-embedded (FFPE), Laser Capture Microdissection (LCM), fine needle biopsies or aspirates.
Abstract translation: 描述了使用Sanger Sequencing重新测序，确认或验证下一代测序（NGS）结果的方法，组合物和试剂盒。 这些方法对于具有非常有限量的样品特别有用，例如福尔马林固定，石蜡包埋（FFPE），激光捕获显微切割（LCM），细针活检或吸出物。
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4.发明申请
公开(公告)号：WO2016053883A1
公开(公告)日：2016-04-07
申请号：PCT/US2015/052677
申请日：2015-09-28
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： SCHREIBER, Edgar , VARMA, Kamini , ANDERSEN, Mark
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6806 , C12Q1/6853 , C12Q1/6858 , C12Q1/6869 , C12Q2537/143 , C12Q2537/149 , C12Q2537/159 , C12Q2549/119 , C12Q2535/122 , C12Q2565/125 , C12Q2521/325 , C12Q2525/113 , C12Q2525/125 , C12Q2535/101
Abstract: In some embodiments, the disclosure relates generally to methods, compositions, systems, apparatuses and kits for performing a first amplification reaction which includes a multiplex nucleic acid amplification reaction using a plurality of target-specific primers in the presence of polymerase under amplification conditions to produce a plurality of amplified target sequences, and sequencing at least some of the amplified target sequences using a gel electrophoresis procedure, for example Sanger sequencing.
Abstract translation: 在一些实施方案中，本公开一般涉及用于进行第一扩增反应的方法，组合物，系统，装置和试剂盒，其包括在扩增条件下在聚合酶存在下使用多种靶特异性引物的多重核酸扩增反应以产生 多个扩增的靶序列，以及使用凝胶电泳方法（例如Sanger测序）测序至少一些扩增的靶序列。
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公开(公告)号：WO2021168478A1
公开(公告)日：2021-08-26
申请号：PCT/US2021/070163
申请日：2021-02-18
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： LI, Kelly , PAGANI, Ioanna , BRZOSKA, Pius , LI, Jisheng , WANG, Chunling , HAYASHIBARA, Kathleen , TANNER, Michael , JI, Hong , VARMA, Kamini , HU, Fangqi , TEBBS, Robert
IPC: C12Q1/70
Abstract: Compositions, assays, methods, diagnostic methods, kits, and diagnostic kits are disclosed for the specific and differential detection of SARS-CoV-2 and/or other viruses from samples, including veterinary samples, clinical samples, food samples, forensic sample, environmental samples (e.g., obtained from soil, garbage, sewage, air, water, food processing and manufacturing surfaces, or likewise), or biological sample obtained from a human or non-human animal.
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Patent Agency Ranking
公开(公告)号：WO2019094973A1
公开(公告)日：2019-05-16
申请号：PCT/US2018/060840
申请日：2018-11-13
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： LI, Kelly , PAGANI, Ioanna , LI, Jisheng , PATEL, Sunali , VARMA, Kamini , FONSECA, Jorge , PURI, Nitin , DIAMOND, Evan
IPC: C12Q1/689
CPC classification number: C12Q1/689 , C12Q2600/16
Abstract: Various methods are disclosed for amplifying nucleic acid sequences in a nucleic acid sample. The methods involve forming at least five amplification reaction mixes each including an aliquot from a sample source that includes nucleic acid sequences, using at least five different assays each including a pair of amplification primers, the assays selected from the group of assays in Table 1and/or targeting the sequences specified in Table 1.
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公开(公告)号：EP4281589A1
公开(公告)日：2023-11-29
申请号：EP22704143.1
申请日：2022-01-25
Applicant: Life Technologies Corporation
Inventor： BRZOSKA, Pius , WILDE, Joyce , FURTADO, Manohar , KENDRICK, Sara , ULEKLEIV, Camilla , STEVENS, Junko , VARMA, Kamini , HAYASHIBARA, Kathleen
IPC: C12Q1/70 , C12Q1/6888
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公开(公告)号：EP3201356A1
公开(公告)日：2017-08-09
申请号：EP15781002.9
申请日：2015-09-28
Applicant: Life Technologies Corporation
Inventor： SCHREIBER, Edgar , VARMA, Kamini , ANDERSEN, Mark
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6806 , C12Q1/6853 , C12Q1/6858 , C12Q1/6869 , C12Q2537/143 , C12Q2537/149 , C12Q2537/159 , C12Q2549/119 , C12Q2535/122 , C12Q2565/125 , C12Q2521/325 , C12Q2525/113 , C12Q2525/125 , C12Q2535/101
Abstract: Methods, compositions and kits are described for resequencing, confirming or verifying Next Generation Sequencing (NGS) results with Sanger Sequencing. These methods are particularly useful for samples having very limited quantities such as formalin-fixed, paraffin-embedded (FFPE), Laser Capture Microdissection (LCM), fine needle biopsies or aspirates.
Abstract translation: 在一些实施方案中，本公开一般涉及用于在扩增条件下在聚合酶存在下使用多个靶特异性引物进行第一扩增反应的方法，组合物，系统，装置和试剂盒，所述第一扩增反应包括多重核酸扩增反应以产生 多个扩增的靶序列，并使用凝胶电泳程序，例如Sanger测序对至少一些扩增的靶序列进行测序。
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公开(公告)号：EP4056719A1
公开(公告)日：2022-09-14
申请号：EP22162324.2
申请日：2021-02-18
Applicant: Life Technologies Corporation
Inventor： LI, Kelly , PAGANI, Ioanna , BRZOSKA, Pius , LI, Jisheng , WANG, Chunling , HAYASHIBARA, Kathleen , TANNER, Michael , JI, Hong , VARMA, Kamini , HU, Fangqi , TEBBS, Robert
IPC: C12Q1/70
Abstract: Compositions, assays, methods, diagnostic methods, kits, and diagnostic kits are disclosed for the specific and differential detection of SARS-CoV-2 and/or other viruses from samples, including veterinary samples, clinical samples, food samples, forensic sample, environmental samples (e.g., obtained from soil, garbage, sewage, air, water, food processing and manufacturing surfaces, or likewise), or biological sample obtained from a human or non-human animal.
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公开(公告)号：EP3472349A1
公开(公告)日：2019-04-24
申请号：EP17733322.6
申请日：2017-06-16
Applicant: Life Technologies Corporation
Inventor： PAGANI, Ioanna , BRZOSKA, Pius , LI, Kelly , PATEL, Sunali , HUANG, Boli , VARMA, Kamini , PURI, Nitin , DIAMOND, Evan
IPC: C12Q1/68
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