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公开(公告)号：ES2727785T3
公开(公告)日：2019-10-18
申请号：ES04733682
申请日：2004-05-18
Applicant: TORAY INDUSTRIES
Inventor： NAGINO KUNIHISA , NAKAMURA FUMIO , NOBUMASA HITOSHI
Abstract: Un soporte que tiene inmovilizada una sustancia de unión selectiva, donde el soporte tiene una parte con una depresión que incluye múltiples proyecciones, la superficie del soporte comprende una resina de autofluorescencia baja que tiene una intensidad de fluorescencia de 1.000 o menos, la superficie de la resina comprende grupos carboxilo formados mediante el tratamiento de la resina con un álcali o ácido, y adicionalmente se inmoviliza una sustancia de unión selectiva sobre las superficies superiores de las múltiples proyecciones de la parte con una depresión que incluye múltiples proyecciones mediante los grupos carboxilo de la resina, donde el soporte tiene formada un área plana, y la diferencia de altura entre la superficie superior plana de las proyecciones de la parte con una depresión que incluye múltiples proyecciones y la superficie del área plana es de 50 μm o menos. donde la intensidad de fluorescencia de la resina de autofluorescencia baja se determina midiendo una placa plana limpia que tiene un grosor de 1 mm utilizando un GenePix 4000B fabricado por Axon Instruments en condiciones de una longitud de onda de excitación de 532 nm, una ganancia de fotomultiplicador fijada en 700 y una potencia de láser del 33 %.
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公开(公告)号：DK1729136T3
公开(公告)日：2012-04-02
申请号：DK05720981
申请日：2005-03-17
Applicant: TORAY INDUSTRIES
Inventor： TAKII YUKI , NAGINO KUNIHISA , NAKAMURA FUMIO , NOBUMASA HITOSHI
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公开(公告)号：CA2883499C
公开(公告)日：2020-07-14
申请号：CA2883499
申请日：2013-08-29
Applicant: TORAY INDUSTRIES
Inventor： HIRANO TAISUKE , NAKAMURA FUMIO , UEDA YOJI , FUJIMOTO KENZO
IPC: C12Q1/6832 , C12Q1/6813
Abstract: A method for detecting a target nucleic acid, which enables highly sensitive detection of the target nucleic acid without lowering the detection sensitivity (S/N ratio) even by use of a reduced amount of a detection probe, is disclosed. The method for detecting a target nucleic acid is based on sandwich hybridization using a detection probe that hybridizes with the target nucleic acid, and a capture probe immobilized on a support. In this method, at least one nucleic acid base in the nucleic acid molecule(s) of the detection probe and/or capture probe is substituted with a photoreactive group. The method comprises the step of irradiating, with light, a complex formed by hybridization between the target nucleic acid and the detection probe and/or capture probe to allow formation of a covalent bond(s) between the photoreactive group(s) and a nucleic acid base(s) in the target nucleic acid.
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公开(公告)号：CA2865541C
公开(公告)日：2019-11-05
申请号：CA2865541
申请日：2013-02-27
Applicant: TORAY INDUSTRIES
Inventor： UEDA YOJI , NAKAMURA FUMIO
IPC: C12N15/09
Abstract: Disclosed is a method for detecting nucleic acid with high sensitivity even in cases where the target nucleic acid is detected by sandwich hybridization using neither nucleic acid amplification nor a sensitization technique. The method for detecting a target nucleic acid comprises the steps of: sequentially or simultaneously bringing a target nucleic acid or fragmentation product thereof, a plurality of detection probes, and a capture probe immobilized on a support, into contact with each other to hybridize the capture probe with the target nucleic acid or fragmentation product thereof and to hybridize the target nucleic acid or fragmentation product thereof with the plurality of detection probes, thereby binding the plurality of detection probes to the support through the capture probe and the target nucleic acid or fragmentation product thereof; and then detecting the plurality of detection probes bound to the support.
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公开(公告)号：DK1626276T3
公开(公告)日：2019-07-29
申请号：DK04733682
申请日：2004-05-18
Applicant: TORAY INDUSTRIES
Inventor： NAGINO KUNIHISA , NAKAMURA FUMIO , NOBUMASA HITOSHI
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Patent Agency Ranking
公开(公告)号：CA2883499A1
公开(公告)日：2014-03-06
申请号：CA2883499
申请日：2013-08-29
Applicant: TORAY INDUSTRIES
Inventor： HIRANO TAISUKE , NAKAMURA FUMIO , UEDA YOJI , FUJIMOTO KENZO
Abstract: Disclosed is a method for detecting a target nucleic acid, which can detect the target nucleic acid with high sensitivity without deteriorating detection sensitivity (an S/N ratio) even when the amount of a detection probe to be used is reduced. A method for detecting a target nucleic acid by a sandwich hybridization method using a detection probe capable of hybridizing with the target nucleic acid and a capture probe immobilized on a support, wherein at least one nucleotide in a nucleic acid molecule of the detection probe and/or the capture probe is substituted by a photoreactive group. The method involves a step of irradiating a complex, which is formed by the hybridization of the target nucleic acid with the detection probe and/or the capture probe, with light to thereby form a covalent bond between the photoreactive group and the nucleotide in the target nucleic acid.
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公开(公告)号：CA2559768C
公开(公告)日：2014-01-07
申请号：CA2559768
申请日：2005-03-17
Applicant: TORAY INDUSTRIES
Inventor： TAKII YUKI , NAGINO KUNIHISA , NAKAMURA FUMIO , NOBUMASA HITOSHI
IPC: G01N35/02 , B01F33/40 , C12M1/02 , C12N15/09 , C12Q1/68 , G01N1/36 , G01N33/53 , G01N35/00 , G01N37/00
Abstract: The method of stirring a solution according to the present invention is a method of stirring a solution comprising contacting a selective binding substance immobilized on the surface of a carrier with a solution containing an analyte substance reactive with the selective binding substance, and mixing the fine particles or air bubbles into the solution containing an analyte substance, and moving the fine particles or air bubbles without allowing contact thereof with the selective binding substance-immobilized surface. The present invention provides a stirring method that accelerates the reaction of a carrier-immobilized selective binding substance with an analyte substance and detects a trace amount of analyte at high signal intensity and high S/N ratio. The present invention enables diagnosis and examination in the clinical setting by using a selective binding substance-immobilized carrier such as DNA chip.
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公开(公告)号：EP2843047A4
公开(公告)日：2015-11-18
申请号：EP13755881
申请日：2013-02-27
Applicant: TORAY INDUSTRIES
Inventor： UEDA YOJI , NAKAMURA FUMIO
CPC classification number: C12Q1/6837 , C12Q1/6827 , C12Q2537/125 , C12Q2545/114
Abstract: Disclosed is a method for detecting nucleic acid with high sensitivity even in cases where the target nucleic acid is detected by sandwich hybridization using neither nucleic acid amplification nor a sensitization technique. The method for detecting a target nucleic acid comprises the steps of: sequentially or simultaneously bringing a target nucleic acid or fragmentation product thereof, a plurality of detection probes, and a capture probe immobilized on a support, into contact with each other to hybridize the capture probe with the target nucleic acid or fragmentation product thereof and to hybridize the target nucleic acid or fragmentation product thereof with the plurality of detection probes, thereby binding the plurality of detection probes to the support through the capture probe and the target nucleic acid or fragmentation product thereof; and then detecting the plurality of detection probes bound to the support.
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公开(公告)号：EP2865764A4
公开(公告)日：2016-03-23
申请号：EP13807796
申请日：2013-06-19
Applicant: TORAY INDUSTRIES
Inventor： NAKAMURA FUMIO , UEDA YOJI , ARIKE TAKAFUMI
CPC classification number: C12Q1/6806 , C12Q1/6825 , C12Q1/6834 , C12Q2527/137 , C12Q2563/131 , C12Q2563/137
Abstract: Disclosed is a method for detecting a nucleic acid using a substance that suppresses, in the labeling step of the post-staining method, detachment of a target nucleic acid that has once hybridized with a capture probe immobilized on a support, which method enables detection of the target nucleic acid with a sensitivity equivalent to or higher than that achieved by a method using sodium ion even in cases where the substance is used at a lower concentration. The method for detecting a nucleic acid comprises the steps of: (1) hybridizing a capture probe with a target nucleic acid to form a double-stranded nucleic acid; bringing the formed double-stranded nucleic acid into contact with a solution containing a labeling substance and a divalent metal cation at a concentration of not less than 10 mM to introduce the labeling substance into the double-stranded nucleic acid; and detecting the labeling substance introduced into the double-stranded nucleic acid.
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公开(公告)号：EP1626276A4
公开(公告)日：2010-05-26
申请号：EP04733682
申请日：2004-05-18
Applicant: TORAY INDUSTRIES
Inventor： NAGINO KUNIHISA , NAKAMURA FUMIO , NOBUMASA HITOSHI
CPC classification number: B01J19/0046 , B01J2219/00497 , B01J2219/00509 , B01J2219/00576 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00677 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01J2219/00731 , Y10S530/812 , Y10S530/815 , Y10S530/816
Abstract: A support carrying an immobilized selective binding substance, that the support surface has a polymer containing the structural unit represented by the following General Formula (1) in an amount of 10% or more with respect all monomer units, and a selective binding substance is immobilized on the support surface by binding to the carboxyl group formed thereon via a covalent bond: (in General Formula (1), R 1 , R 2 , and R 3 each represent an alkyl or aryl group or a hydrogen atom.)
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