公开(公告)号：WO2018022776A1

公开(公告)日：2018-02-01

申请号：PCT/US2017/043989

申请日：2017-07-26

Applicant: IMRA AMERICA, INC.

Inventor： MILLS, Andrew, A. ,  CEDERQUIST, Kristin, B. ,  LIU, Bing

IPC: C08G65/328 ,  G01N33/20

Abstract: Disclosed is a method of pulsed laser ablation for producing gold-platinum AuxPti-x alloy nanoparticles in a colloidal solution. The resulting colloidal solution of AuxPti-x alloy nanoparticles is suitable for a variety of biological applications including lateral flow immunoassays and other bio-detections based on optical scattering of metal nanoparticles. In the present method, the laser pulse duration is kept in the picosecond to femtosecond region, which is sufficiently short to ablate the target without significantly heating the target material. The nanoparticles form by fragmentation of the bulk material without evaporation, minimizing oxidation of the nanoparticles. The nanoparticles conjugate with bio-molecules such as protein, antibodies, peptides, RNA oligomers, DNA oligomers, other oligomers, or polymers effectively by passive adsorption. This process of bio-molecule conjugation is the same as that with pure gold nanoparticles, and will not require significant changes in the fabrication protocols for bioassay and bio-detection device manufacturers. Advantageously the AuxPti-x alloy nanoparticles have a wide optical extinction spectrum in the visible region, appearing nearly black in both colloidal and dried form. The nanoparticles can be used for labeling bio-molecules and provide a high visual contrast in visual-based bioassays such as lateral flow immunoassays against the white test paper strips. A combination of the near black color of the AuxPti-x alloy nanoparticles with the red color of pure Au nanoparticles makes multiplexing bio-detection assays possible.

Abstract translation: 公开了一种用于在胶体溶液中产生金 - 铂AuxPti-x合金纳米颗粒的脉冲激光烧蚀方法。 得到的AuxPti-x合金纳米颗粒胶体溶液适用于各种生物应用，包括横向流动免疫分析和基于金属纳米颗粒光散射的其他生物检测。 在本方法中，激光脉冲持续时间保持在皮秒至飞秒区域，该区域足够短以消融目标而不显着加热目标材料。 纳米颗粒通过大块材料的碎裂形成而不蒸发，从而使纳米颗粒的氧化最小化。 纳米粒子与生物分子如蛋白质，抗体，肽，RNA寡聚物，DNA寡聚物，其他寡聚物或聚合物通过被动吸附有效地结合。 这种生物分子结合过程与纯金纳米粒子结合的过程相同，并且不需要对生物测定和生物检测设备制造商的制造方案进行重大改变。 有利地，AuxPti-x合金纳米颗粒在可见光区具有宽的消光光谱，在胶体和干燥形式下都呈现接近黑色。 纳米粒子可用于标记生物分子，并在基于视觉的生物分析中提供高度的视觉对比度，例如针对白色试纸条的横向流动免疫分析。 AuxPti-x合金纳米粒子的接近黑色与纯金纳米粒子的红色组合使得多重生物检测分析成为可能。

公开(公告)号：WO2016048740A2

公开(公告)日：2016-03-31

申请号：PCT/US2015/050362

申请日：2015-09-16

Applicant: IMRA AMERICA, INC.

Inventor： FERMANN, Martin, E. ,  KUSE, Naoya ,  LEE, Kevin, F.

IPC: H01S3/105

CPC classification number: H01S3/1112 ,  H01S3/06758 ,  H01S3/094003 ,  H01S3/0941 ,  H01S3/10061 ,  H01S3/105 ,  H01S3/106 ,  H01S3/1067 ,  H01S3/107 ,  H01S3/108 ,  H01S3/1115 ,  H01S3/1118 ,  H01S3/1305 ,  H01S3/1312 ,  H01S3/139 ,  H01S3/1608 ,  H01S3/2308 ,  H01S2301/02

Abstract: The present disclosure relates to the design of fiber frequency comb lasers with low carrier phase noise. Examples of these low carrier phase noise oscillators can be constructed from both soliton and dispersion compensated fiber lasers via the use of intra-cavity amplitude modulators such as graphene modulators. In low carrier phase noise dispersion compensated fiber frequency comb lasers, graphene and/or bulk modulators can further be used, for example, for phase locking of one comb line to an external continuous wave (cw) reference laser via high bandwidth control of the repetition rate of the comb laser via the graphene modulator. As a result a low phase noise radio frequency (RF) signal can be generated. In some implementations, a frequency comb exhibiting phase noise suppression of at least about 10 dB over a frequency range up to about 100 kHz is provided.

Abstract translation: 本公开涉及具有低载波相位噪声的光纤频梳激光器的设计。 这些低载波相位噪声振荡器的例子可以由孤子和色散补偿光纤激光器通过使用腔内振幅调制器如石墨烯调制器来构建。 在低载波相位噪声色散补偿光纤频梳激光器中，例如可以进一步使用石墨烯和/或体调制器，用于通过重复的高带宽控制将一条梳状线锁相至外部连续波（cw）参考激光器 梳状激光通过石墨烯调制器的速率。 结果可以产生低相位噪声射频（RF）信号。 在一些实施方式中，提供了频率梳，该频率梳在高达大约100kHz的频率范围内呈现至少约10dB的相位噪声抑制。

公开(公告)号：WO2015183659A1

公开(公告)日：2015-12-03

申请号：PCT/US2015/031731

申请日：2015-05-20

Applicant: IMRA AMERICA, INC.

Inventor： QIAN, Wei ,  LIU, Bing ,  FETTERS, Kori, Michael

IPC: G01N33/58 ,  C12Q1/37

CPC classification number: C12Q1/37 ,  G01N33/587 ,  G01N2333/81 ,  G01N2333/948

Abstract: In the present invention, a method and assay for the detection of proteases and protease inhibitors using colloidal gold nanoparticles and peptide substrates, which are selectively recognized and cleaved by proteases being assayed, is disclosed. In this assay, the mechanism of signal generation relies on peptide sequence induced aggregation of gold nanoparticles, which are used as signal reporters. The peptide sequences that induce aggregation are either the intact peptide substrates or proteolytic fragments of the intact peptide substrate wherein the proteolytic fragments are produced by the protease being assayed. The present invention provides a novel, simple, sensitive, and inexpensive colloidal gold nanoparticle-based colorimetric assay that allows both visual and quantitative detection of proteases and protease inhibitors.

Abstract translation: 在本发明中，公开了使用被测定蛋白酶选择性识别和切割的胶体金纳米颗粒和肽底物检测蛋白酶和蛋白酶抑制剂的方法和测定法。 在该测定中，信号产生的机制依赖于用作信号记录器的金纳米颗粒的肽序列诱导的聚集。 诱导聚集的肽序列是完整肽底物的完整肽底物或蛋白水解片段，其中蛋白水解片段由待测蛋白酶产生。 本发明提供了一种新颖，简单，灵敏和便宜的基于胶体金纳米颗粒的比色测定法，其允许视觉和定量检测蛋白酶和蛋白酶抑制剂。

公开(公告)号：WO2015138019A1

公开(公告)日：2015-09-17

申请号：PCT/US2014/069535

申请日：2014-12-10

Applicant: IMRA AMERICA,INC.

Inventor： TAN, Bing ,  HU, Zhendong ,  HE, Guanghui ,  CHE, Yong

IPC: H01M4/48

CPC classification number: H01M4/485 ,  H01M10/052 ,  H01M2004/021 ,  H01M2004/027

Abstract: The described embodiments provide an energy storage device that includes a positive electrode including an active material that can store and release ions, a negative electrode including a V, Nb co-doped TiO 2 (B), and a non-aqueous electrolyte including lithium ions. At least one embodiment provides a negative electrode active material including V, Nb co-doped TiO 2 (B). At least one embodiment provides a wet-chemistry process to prepare V, Nb co-doped TiO 2 (B).

Abstract translation: 所描述的实施例提供了一种能量存储装置，其包括包括可以存储和释放离子的活性材料的正极，包括V，Nb共掺杂TiO 2（B）的负极和包含锂离子的非水电解质。 至少一个实施方案提供了包括V，Nb共掺二氧化钛（B）的负极活性材料。 至少一个实施方案提供了制备V，Nb共掺杂TiO 2（B）的湿化学方法。

公开(公告)号：WO2015130651A1

公开(公告)日：2015-09-03

申请号：PCT/US2015/017245

申请日：2015-02-24

Applicant: IMRA AMERICA, INC.

Inventor： HORI, Takashi ,  FERMANN, Martin, E.

IPC: H01S3/00 ,  H01S3/067 ,  H01S3/16

CPC classification number: H01S3/06758 ,  G02F1/39 ,  G02F2001/392 ,  H01S3/005 ,  H01S3/0057 ,  H01S3/0085 ,  H01S3/0092 ,  H01S3/06708 ,  H01S3/1106 ,  H01S3/1608 ,  H01S3/1611 ,  H01S3/1616 ,  H01S3/1618 ,  H01S3/302 ,  H01S2301/08 ,  H01S2301/085

Abstract: In one aspect, the present disclosure describes a fiber laser system for the generation and delivery of femtosecond (fs) pulses in multiple wavelength ranges. For improved versatility in multi-photon microscopy, an example of a dual wavelength fiber system based on Nd fiber source providing gain at 920 and 1060 nm is described. An example of a three-wavelength system is included providing outputs at 780 nm, 940 nm, and 1050 nm. The systems include dispersion compensation so that high quality fs pulses are provided for applications in microscopy, for example in multiphoton microscope (MPM) systems.

Abstract translation: 一方面，本公开描述了用于在多个波长范围内产生和传送飞秒（fs）脉冲的光纤激光系统。 为了改善多光子显微镜的多功能性，描述了基于提供920和1060nm的增益的Nd光纤源的双波长光纤系统的示例。 包括三波长系统的实例包括提供780nm，940nm和1050nm的输出。 这些系统包括色散补偿，以便提供高质量的fs脉冲用于显微镜应用，例如在多光子显微镜（MPM）系统中。

公开(公告)号：WO2015108991A2

公开(公告)日：2015-07-23

申请号：PCT/US2015/011420

申请日：2015-01-14

Applicant: IMRA AMERICA, INC.

Inventor： OTA, Michiharu ,  ARAI, Alan, Y. ,  LIU, Zhenlin

IPC: B23K26/36

CPC classification number: B23K26/0624 ,  B23K26/0604 ,  B23K26/082 ,  B23K26/36 ,  B23K26/364 ,  B23K26/38 ,  B23K26/382 ,  B23K26/40 ,  B23K26/402 ,  B23K2203/50 ,  B23K2203/54 ,  C03B33/102

Abstract: In certain embodiments a method and system for laser-based material processing of a material is disclosed. In at least one preferred implementation temporally overlapping pulse series are generated with separate pulsed laser sources, for example nanosecond (NS) and ultrashort pulse (USP) sources (NS-USP). Pulses are delivered to the material as a series of spatially and temporally overlapping pulse pairs. The material can, but need not, be a transparent material. In some applications of transparent material processing, it was found the combination of pulses both substantially more material modification and high machining quality than obtainable with either individual pulse series taken alone. Other micromachining methods and arrangement are disclosed for formation of fine features on or within a substrate. Such methods and arrangements may generally be applied with a NS-USP combination, or with other sources.

Abstract translation: 在某些实施例中，公开了一种用于材料的基于激光的材料处理的方法和系统。 在至少一个优选的实施方案中，使用单独的脉冲激光源（例如纳秒（NS））和超短脉冲（USP）源（NS-USP）产生时间上重叠的脉冲序列。 脉冲作为一系列空间和时间重叠的脉冲对传送到材料。 材料可以但不一定是透明材料。 在透明材料加工的一些应用中，发现脉冲的组合比单独采用的单个脉冲系列可获得的材料变化和高加工质量要大得多。 公开了其他微加工方法和布置用于在基底上或内部形成精细特征。 这种方法和装置通常可以用NS-USP组合或与其他来源一起使用。

公开(公告)号：WO2014116767A3

公开(公告)日：2014-07-31

申请号：PCT/US2014/012636

申请日：2014-01-23

Applicant: IMRA AMERICA, INC.

Inventor： ICHIKAWA, Yuki ,  MARCINKEVICUS, Andrius ,  ITO, Masayuki ,  QIAN, Wei

IPC: B01J13/00

Abstract: The present disclosure is directed to methods of preparing stable suspensions of precious metal nanoparticles and methods for attaching bio-molecules to the nanoparticles. The formation of nanoparticles can be accomplished by either chemical synthesis or pulsed laser ablation in a liquid. The present disclosure reveals the importance of controlling the conductivity of the dispersion medium during pulsed laser ablation in a liquid to control the particle size of the nanoparticles. The present disclosure also reveals the importance of adjusting and maintaining the conductivity in a range of 25 μS/cm or less during storage of the nanoparticles and just prior to performing bioconjugation reactions. The control of conductivity is an important process for maintaining the nanoparticles as a stable non-aggregated colloidal suspension in a dispersion medium.

公开(公告)号：WO2014062759A2

公开(公告)日：2014-04-24

申请号：PCT/US2013/065169

申请日：2013-10-16

Applicant: IMRA AMERICA, INC.

Inventor： FERMANN, Martin

IPC: G02F1/35

CPC classification number: H01S3/1106 ,  B23K26/0624 ,  H01J49/161 ,  H01S3/0057 ,  H01S3/06708 ,  H01S3/06741 ,  H01S3/0675 ,  H01S3/06754 ,  H01S3/094003 ,  H01S3/094026 ,  H01S3/094042 ,  H01S3/094061 ,  H01S3/0941 ,  H01S3/109 ,  H01S3/1115

Abstract: The present invention relates to compact, low noise, ultra-short pulse sources based on fiber amplifiers, and various applications thereof. At least one implementation includes an optical amplification system having a fiber laser seed source producing seed pulses at a repetition rate corresponding to the fiber laser cavity round trip time. A nonlinear pulse transformer, comprising a fiber length greater than about 10 m, receives a seed pulse at its input and produces a spectrally broadened output pulse at its output, the output pulse having a spectral bandwidth which is more than 1.5 times a spectral bandwidth of a seed pulse. A fiber power amplifier receives and amplifies spectrally broadened output pulses. A pulse compressor is configured to temporally compress spectrally broadened pulses amplified by said power amplifier. Applications include micro-machining, ophthalmology, molecular desorption or ionization, mass-spectroscopy, and/or laser- based, biological tissue processing.

Abstract translation: 本发明涉及基于光纤放大器的紧凑，低噪声，超短脉冲源及其各种应用。 至少一个实施方案包括具有光纤激光种子源的光放大系统，其以对应于光纤激光腔往返时间的重复频率产生种子脉冲。 包括光纤长度大于约10微米的非线性脉冲变压器在其输入处接收种子脉冲，并在其输出端产生光谱扩展的输出脉冲，其输出脉冲的频谱带宽大于光谱带宽的1.5倍 种子脉冲。 光纤功率放大器接收并放大光谱扩展的输出脉冲。 脉冲压缩器被配置为在时间上压缩由所述功率放大器放大的频谱变宽的脉冲。 应用包括微加工，眼科学，分子解吸或离子化，质谱法和/或基于激光的生物组织处理。

公开(公告)号：WO2013063090A3

公开(公告)日：2014-03-13

申请号：PCT/US2012061656

申请日：2012-10-24

Applicant: IMRA AMERICA INC

Inventor： HAGEDORN KEVIN V ,  LIU BING

IPC: C25D13/02 ,  B32B5/16 ,  B82Y30/00 ,  C25D13/10

CPC classification number: C25D13/02 ,  B82Y30/00 ,  C25D13/10 ,  Y10T428/25 ,  Y10T428/31678

Abstract: Disclosed is a process for electrophoretic deposition of colloidal suspensions of nanoparticles, especially from aprotic solvents, onto a variety of substrates. The process provides chemical additives that can be used to improve thin films deposited from colloidal suspensions by increasing the rate of deposition and the smoothness of the deposited film. In this process, a chemical additive is used to improve the properties of the deposited thin films. The chemical additive comprises a redox couple, an organometallic complex, a metallocene, a ferrocene, or a nickelocene. The colloidal suspension can be composed of semiconductor, metal or ceramic nanoparticles suspended in an aprotic polar solvent such as acetone, acetonitrile, or pyridine. The process also improves the properties of thin films deposited from protic solvents. The particles have at least one dimension ranging from 0.1 nanometers (nm) to 500 nm.

Abstract translation: 公开了一种将纳米粒子，特别是非质子溶剂的胶体悬浮液电泳沉积到各种基底上的方法。 该方法提供化学添加剂，其可用于通过增加沉积速率和沉积膜的平滑度来改善由胶体悬浮液沉积的薄膜。 在该方法中，使用化学添加剂来改善沉积的薄膜的性质。 化学添加剂包括氧化还原对，有机金属络合物，茂金属，二茂铁或二茂镍。 胶态悬浮液可以由悬浮在非质子极性溶剂如丙酮，乙腈或吡啶中的半导体，金属或陶瓷纳米颗粒组成。 该方法还改善了由质子溶剂沉积的薄膜的性质。 颗粒具有0.1纳米（nm）至500nm的至少一个维度。

公开(公告)号：WO2013165945A1

公开(公告)日：2013-11-07

申请号：PCT/US2013/038752

申请日：2013-04-30

Applicant: IMRA AMERICA, INC.

Inventor： FERMANN, Martin E. ,  HARTL, Ingmar

IPC: H01S3/05 ,  H01S3/098 ,  H01S3/10

CPC classification number: G02F1/39 ,  H01S3/0092 ,  H01S3/2308 ,  H01S3/2383

Abstract: The present invention relates to frequency rulers. At least one embodiment includes a mode locked pump source operated at pulse repetition rate, and a pump output having a pump carrier envelope offset frequency. A nonlinear optical system outputs a frequency ruler spectrum comprising individual frequency modes. The frequency modes may be characterized by a frequency spacing which is an integer multiple of the repetition rate and by distinct ruler carrier envelope offset frequencies which exhibit at least one discontinuity across the frequency output. The ruler carrier envelope offset frequencies are substantially locked to the carrier envelope offset frequency of the pump laser. One preferred embodiment includes a frequency doubled, doubly resonant, non-degenerate OPO (DNOPO), a supercontinuum generation (SC) stage and at least one reference laser arranged downstream from a Tm fiber-based pump source. A plurality of beat signals generated therefrom provide for stabilization of the system.

Abstract translation: 本发明涉及频率标尺。 至少一个实施例包括以脉冲重复率操作的模式锁定泵浦源，以及具有泵载波包络偏移频率的泵输出。 非线性光学系统输出包括各种频率模式​​的频率标尺。 频率模式的特征在于频率间隔是重复率的整数倍，并且通过不同的标尺载波包络偏移频率来表征，该频率间隔在频率输出端表现出至少一个不连续性。 标尺载波包络偏移频率基本上锁定在泵浦激光器的载波包络偏移频率上。 一个优选实施例包括频率倍增，双重谐振，非简并OPO（DNOPO），超连续谱产生（SC）级以及布置在基于Tm纤维的泵浦源的下游的至少一个参考激光器。 由此产生的多个拍子信号提供了系统的稳定性。