公开(公告)号：US20030179372A1

公开(公告)日：2003-09-25

申请号：US10393607

申请日：2003-03-21

Applicant: Leica Microsystems Heidelberg GmbH

Inventor： Werner Knebel

IPC: G01N021/64

CPC classification number: G01N21/6458 ,  G01J3/1256 ,  G01J3/32 ,  G01J3/36 ,  G01N2021/6421 ,  G02B21/0064 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/16 ,  G02B21/365

Abstract: A method for spectral analysis of the light proceeding from a specimen using a multi-band detector comprises the steps of defining an overall spectral region; from the overall spectral region, defining a first spectral subregion and defining at least a second spectral subregion; simultaneously detecting the light proceeding from the specimen in the first and the second spectral subregion, and generating detection values; displacing the first spectral subregion and displacing the second spectral subregion within the overall spectral region; and repeating steps c) and d) until the light has been detected over the entire overall spectral region.

Abstract translation: 用于使用多频带检测器从样本进行光的光谱分析的方法包括以下步骤：定义整个光谱区域; 从整个光谱区域，定义第一光谱子区域并限定至少第二光谱子区域; 同时检测从第一和第二频谱子区域中的样本进行的光，并产生检测值; 移位所述第一频谱子区域并且移位所述整个频谱区域内的所述第二频谱子区域; 并重复步骤c）和d），直到在整个光谱区域上检测到光。

公开(公告)号：US06624889B1

公开(公告)日：2003-09-23

申请号：US10135256

申请日：2002-04-29

Applicant: Shifang Li

Inventor： Shifang Li

IPC: G01J400

CPC classification number: G01J3/1256 ,  G01J3/447 ,  H04J14/02 ,  H04J14/06

Abstract: An optical channel monitor (OCM) or filter for analyzing an incident light carrying a number of narrow band signal channels such as WDM or DWDM channels. The OCM or filter use an acousto-optic tunable filter to receive and refract from an incident light a refracted light such that the refracted light contains a test channel with a center frequency &ngr;0. A first birefringent element is provided for filtering from the refracted light a first polarized light and a second polarized light orthogonal to the first polarized light. The transmission curves are engineered such that the transmissions of the first and second polarized light are substantially equal at the center frequency &ngr;0 of the test channel. The OCM or filter has a second birefringent element for filtering from the first polarized light a first polarized portion and a second polarized portion. The transmission curves of the second birefringent element are set such that the transmissions of the first and second polarized portions are substantially equal at a first offset &dgr;1&ngr; from the center frequency &ngr;0.

Abstract translation: 用于分析携带许多窄带信号通道（如WDM或DWDM通道）的入射光的光通道监视器（OCM）或滤波器。 OCM或滤波器使用声光可调谐滤波器从入射光接收和折射折射光，使得折射光包含具有中心频率nu0的测试通道。 提供了第一双折射元件，用于从折射光中滤出与第一偏振光正交的第一偏振光和第二偏振光。 传输曲线被设计成使得第一和第二偏振光的传输在测试通道的中心频率nu0处基本相等。 OCM或滤波器具有用于从第一偏振光第一偏振部分和第二偏振部分滤波的第二双折射元件。 第二双折射元件的透射曲线被设定为使得第一和第二偏振部分的透射在与中心频率nu0相距的第一偏移量Δ1nu处基本相等。

公开(公告)号：US6007996A

公开(公告)日：1999-12-28

申请号：US122704

申请日：1998-07-27

Applicant: George McNamara ,  Dirk Soenksen ,  Dario Cabib ,  Robert A. Buckwald

Inventor： George McNamara ,  Dirk Soenksen ,  Dario Cabib ,  Robert A. Buckwald

IPC: G01B11/00 ,  A61B3/00 ,  A61B3/12 ,  A61B5/00 ,  A61B10/00 ,  C12Q1/68 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/443 ,  G01J3/45 ,  G01J3/453 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G02B21/36 ,  G06K9/00 ,  G06K9/76 ,  G01N33/53

CPC classification number: G01N21/6458 ,  C12Q1/6841 ,  C12Q1/6883 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G06K9/00127 ,  G06K9/76 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6417 ,  G01N2021/6423

Abstract: A method of in situ analysis of a biological sample comprising the steps of (a) staining the biological sample with N stains of which a first stain is selected from the group consisting of a first immunohistochemical stain, a first histological stain and a first DNA ploidy stain, and a second stain is selected from the group consisting of a second immunohistochemical stain, a second histological stain and a second DNA ploidy stain, with provisions that N is an integer greater than three and further that (i) if the first stain is the first immunohistochemical stain then the second stain is either the second histological stain or the second DNA ploidy stain; (ii) if the first stain is the first histological stain then the second stain is either the second immunohistochemical stain or the second DNA ploidy stain; whereas (iii) if the first stain is the first DNA ploidy stain then the second stain is either the second immunohistochemical stain or the second histological stain; and (b) using a spectral data collection device for collecting spectral data from the biological sample, the spectral data collection device and the N stains are selected such that a spectral component associated with each of the N stains is collectable.

Abstract translation: 一种生物样品的原位分析方法，包括以下步骤：（a）染色具有N染色的生物样品，其中第一染色选自第一免疫组织化学染色，第一组织染色和第一DNA倍体 染色，第二染色选自第二免疫组织化学染色，第二组织学染色和第二DNA倍性染色，其规定N为大于3的整数，并且（i）如果第一染色是 第一次免疫组织化学染色，第二次染色是第二次组织染色或第二次DNA倍体染色; （ii）如果第一染色是第一组织染色，则第二染色是第二免疫组织化学染色或第二DNA倍体染色; 而（iii）如果第一个染色是第一个DNA倍性染色，则第二次染色是第二次免疫组化染色或第二次组织染色; 和（b）使用用于收集来自生物样品的光谱数据的光谱数据采集装置，选择光谱数据采集装置和N染色，使得与每个N染色体相关联的光谱分量是可收集的。

公开(公告)号：US5936731A

公开(公告)日：1999-08-10

申请号：US575191

申请日：1995-12-20

Applicant: Dario Cabib ,  Robert A. Buckwald ,  David Wine ,  Yuval Garini ,  Nir Katzir ,  Dirk G. Soeknsen ,  Moshe Lavi

Inventor： Dario Cabib ,  Robert A. Buckwald ,  David Wine ,  Yuval Garini ,  Nir Katzir ,  Dirk G. Soeknsen ,  Moshe Lavi

IPC: G01B9/02 ,  A61B3/12 ,  A61B5/00 ,  C12Q1/68 ,  G01B11/06 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G06K9/00 ,  G06K9/76

CPC classification number: G01N21/6458 ,  A61B3/12 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6883 ,  G01B11/0675 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J3/4535 ,  G01N21/6428 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G06K9/00127 ,  G06K9/76 ,  A61B3/1241 ,  C12Q2600/156 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6417 ,  G01N2021/6441

Abstract: A fluorescent in situ hybridization method comprising the steps of (a) providing a cell nuclei having chromosomes hybridized with at least one nucleic acid probe including at least one nucleic acid molecule labeled with at least one fluorophore; (b) viewing the cell nuclei through a fluorescence microscope optically connected to an imaging spectrometer for obtaining a spectrum of each pixel of the cell nuclei by (i) collecting incident collimated light simultaneously from all pixels of the cell nuclei; (ii) passing the incident collimated light through an interferometer system so that the light is first split into two coherent beams and then recombine to interfere and form an exiting light beam; (iii) focusing the exiting light beam on a detector having an array of detector elements, so that at each instant each of the elements is the image of one and always the same pixel for the entire duration of the measurement and so that each of the elements produces a signal which is a particular linear combination of light intensity emitted by the pixel at different wavelengths; (iv) rotating or translating one or more of the elements of the interferometer, so that the optical path difference is scanned simultaneously for all the pixels of the cell nuclei; and (v) recording signals of each of the detector elements as function of time using a recording device to form a first spectral cube of data; and (c) interpreting the first spectral cube of data using a mathematical algorithm.

Abstract translation: 荧光原位杂交方法包括以下步骤：（a）提供具有染色体的细胞核，所述染色体与至少一种核酸探针杂交，所述核酸探针包括至少一个用至少一个荧光团标记的核酸分子; （b）通过光学连接到成像光谱仪的荧光显微镜观察细胞核，通过（i）从细胞核的所有像素同时收集入射准直光，以获得细胞核的每个像素的光谱; （ii）使入射的准直光通过干涉仪系统，使得光首先分裂成两个相干光束，然后重组以干涉并形成出射的光束; （iii）将出射的光束聚焦在具有检测器元件阵列的检测器上，使得在每个时刻，在整个测量持续时间内，每个元件是一个并且总是相同像素的图像，并且使得每个 元件产生信号，其是由不同波长的像素发射的光强度的特定线性组合; （iv）旋转或平移干涉仪的一个或多个元件，使得对于细胞核的所有像素同时扫描光程差; 以及（v）使用记录装置记录每个检测器元件的信号作为时间的函数，以形成数据的第一光谱立方体; 和（c）使用数学算法解释数据的第一个频谱立方体。

公开(公告)号：US5821536A

公开(公告)日：1998-10-13

申请号：US978133

申请日：1997-11-25

Applicant: John W. Pettit

Inventor： John W. Pettit

IPC: G01J3/12 ,  G01N21/35 ,  G01N21/86

CPC classification number: G01J3/1256 ,  G01N21/3559

Abstract: A solid state moisture gauge is disclosed in which a quartz-halogen bulb is used with an acousto-optic tunable filter to launch light of specific desired wavelengths into a parallel plate cavity device which contains or is adjacent to material having a moisture content which is to be measured. The light exiting from the parallel plate device is detected, converted to a digital signal and then stored in a computer for later comparison to the detection of light exiting from the parallel plate device of a different wavelength.

Abstract translation: 公开了一种固态湿度计，其中石英卤素灯泡与声光可调谐滤光器一起使用以将特定期望波长的光发射到平行板空腔装置中，该平行板腔装置包含或邻近具有水分含量的材料 被测量。 从平行板装置出射的光被检测，转换为数字信号，然后存储在计算机中，以便与从不同波长的平行板装置出射的光的检测进行比较。

公开(公告)号：US5817462A

公开(公告)日：1998-10-06

申请号：US635820

申请日：1996-04-22

Applicant: Yuval Garini ,  Dario Cabib ,  Robert A. Buckwald ,  Thomas Ried ,  Dirk G. Soenksen

Inventor： Yuval Garini ,  Dario Cabib ,  Robert A. Buckwald ,  Thomas Ried ,  Dirk G. Soenksen

IPC: C12N15/09 ,  C12Q1/68 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01N21/64 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G02B21/16 ,  G06K9/00 ,  G06K9/76 ,  C07H23/00 ,  G02B21/00

CPC classification number: G01N21/6452 ,  C12Q1/6841 ,  C12Q1/6883 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G06K9/00127 ,  G06K9/76 ,  C12Q2600/156 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6417 ,  G01N2021/6441

Abstract: A spectral imaging method for simultaneous detection of multiple fluorophores aimed at detecting and analyzing fluorescent in situ hybridizations employing numerous chromosome paints and/or loci specific probes each labeled with a different fluorophore or a combination of fluorophores for color karyotyping, and at multicolor chromosome banding, wherein each chromosome acquires a specifying banding pattern, which pattern is established using groups of chromosome fragments labeled with various fluorophore or combinations of fluorophores.

公开(公告)号：US5737076A

公开(公告)日：1998-04-07

申请号：US473743

申请日：1995-06-07

Applicant: Ulrich Walter Glaus ,  Martin Labhart ,  Heinz Wagner

Inventor： Ulrich Walter Glaus ,  Martin Labhart ,  Heinz Wagner

IPC: G01N21/27 ,  G01J3/12 ,  G01J3/28 ,  G01J3/36 ,  G01J3/457 ,  G01N21/31 ,  G01N21/61 ,  G02B7/00

CPC classification number: G01J3/36 ,  G01J3/28 ,  G01J3/457 ,  G01N21/31 ,  G01J2003/1278 ,  G01J2003/1291 ,  G01J2003/2866 ,  G01J3/1256

Abstract: The invention relates to a procedure for determining an identification of a sample of material, or its properties. Electromagnetic radiation from a radiation source (1) is reflected or transmitted through the sample. The radiation from the sample is collected and analyzed over several channels (7, 7', 7"), which modulate the radiation with a spectral transmission function which is unique for each channel. The modulated radiation is transmitted to one or several detectors (9, 9', 9") which produce output signals which are further electronically processed. The spectral range of each of the individual channels is common to all of the channels. Within the common range the channels are provided with different spectral transmission functions (7, 7',7") which are optimally chosen for a given application.

Abstract translation: 本发明涉及用于确定材料样品或其性质的鉴定的程序。 来自辐射源（1）的电磁辐射被反射或透过样品。 来自样品的辐射在几个通道（7,7'，7“）上进行收集和分析，该通道利用每个通道唯一的光谱传输函数调制辐射。 调制的辐射被传输到一个或多个检测器（9,9'，9“），这些检测器产生进一步电子处理的输出信号。 每个通道的光谱范围对于所有通道是共同的。 在通用范围内，通道被提供有不同的光谱传输功能（7,7'，7“），这些功能对于给定的应用是最佳选择的。

公开(公告)号：US5444528A

公开(公告)日：1995-08-22

申请号：US281411

申请日：1994-07-27

Applicant: Jeffrey J. Puschell

Inventor： Jeffrey J. Puschell

IPC: G01J3/12 ,  G01J3/44 ,  G01N21/65 ,  G01J3/42

CPC classification number: G01J3/44 ,  G01J3/1256

Abstract: A tunable spectrometer includes an acousto-optical tunable filter disposed for filtering light from a discrete light source, wherein the light emerges from the filter in a simultaneous plurality of distinct beams having different orders of diffraction, with different diffraction orders having different wavelength ranges and with complementary pairs of said distinct beams having the same diffraction order respectively having substantially the same wavelength ranges. The filter is tuned to diffract light in a simultaneous plurality of distinct beams having different predetermined diffraction orders that have different wavelength ranges that include wavelengths that are characteristic of a spectrum for a given substance; and a set of a given plural number of detectors respectively detect the filtered light diffracted by the filter to thereby simultaneously provide test signals indicative of a spectrum within a spatial region of interest at wavelengths that are characteristic of the spectrum for the given substance. Another spectrometer that detects light at a wavelength that is characteristic of a Raman scattering spectrum for a given substance includes a test cell disposed within the spatial region of interest and including a plurality of mirrors disposed within the cell for reflecting the light to pass a plurality of round trips between the mirrors before emerging from the spatial region of interest to thereby enhance Raman scattering of the light by any molecules of the given substance within the cell while the light passes through the spatial region of interest.

Abstract translation: 可调谐光谱仪包括设置用于过滤来自离散光源的光的声光可调谐滤光器，其中光从具有不同衍射级的同时多个不同光束的滤光器出射，具有不同的衍射级具有不同的波长范围，并且 具有相同衍射级的所述不同光束的互补对分别具有基本上相同的波长范围。 滤波器被调谐以在具有不同波长范围的不同预定衍射级的同时多个不同的光束中衍射光，其包括对于给定物质的光谱特征的波长; 并且一组给定的多个检测器分别检测由滤波器衍射的滤波光，从而同时提供指示在给定物质的光谱特征的波长处的感兴趣的空间区域内的光谱的测试信号。 检测对于给定物质的拉曼散射光谱特性的波长的光的另一种光谱仪包括设置在感兴趣的空间区域内的测试单元，并且包括设置在单元内的多个反射光，以反射光以通过多个 在从感兴趣的空间区域出射之前，反射镜之间的往返行进，由此在光通过感兴趣的空间区域时，通过细胞内的给定物质的任何分子增强光的拉曼散射。

公开(公告)号：US5434666A

公开(公告)日：1995-07-18

申请号：US201642

申请日：1994-02-25

Applicant: Jon W. Carnahan ,  Brian A. Pelz

Inventor： Jon W. Carnahan ,  Brian A. Pelz

IPC: G01J3/12 ,  G01J3/18 ,  G01J3/20

CPC classification number: G01J3/20 ,  G01J3/1256

Abstract: A spectrometer is adapted to observe discrete wavelengths or spectral regions of a sample. The spectrometer includes a light source having spectral characteristics depending on the presence of a sample. A disperser consists of a chamber housing a diffraction grating in spaced relation to the light source and an entrance slit associated with the chamber for directing light from the source in a light path to be dispersed by the grating. An acousto-optic device is mounted in the chamber in the light path so that light traverses the device. A power supply applies an acoustic frequency to the acousto-optic device to set up a variation in refractive index within the acousto-optic device to deflect traversing light. A detector associated with the chamber is positioned relative to the grating to sense the presence of light where it appears after dispersion and to rapidly detect discrete wavelengths or spectral regions of the light.

Abstract translation: 光谱仪适于观察样品的离散波长或光谱区域。 光谱仪包括具有取决于样品存在的光谱特性的光源。 分散器包括容纳与光源间隔开的衍射光栅的腔室和与腔室相关联的入口狭缝，用于将来自光源的光源引导到由光栅分散的光路中。 声光装置安装在光路中的腔室中，使得光穿过该装置。 电源将声频施加到声光器件以建立声光器件内的折射率变化以偏转横穿光。 与腔室相关联的检测器相对于光栅定位以感测在分散之后出现的光的存在并且快速检测光的离散波长或光谱区域。

公开(公告)号：US5066126A

公开(公告)日：1991-11-19

申请号：US385746

申请日：1989-07-18

Applicant: Masami Hatori

Inventor： Masami Hatori

IPC: G01J1/42 ,  G01J3/00 ,  G01J3/12 ,  G02F1/11 ,  G02F1/125

CPC classification number: G01J3/1256 ,  G01J3/0259 ,  G01J2001/4242 ,  G01N2201/0873

Abstract: An optical spectrum analyzer includes a surface elastic wave generator for generating in an optical waveguide a surface elastic wave which has a continuously varying frequency and which diffracts and deflects a guided wave as it travels across the path of the guided wave, which guided wave is introduced as a measured light beam into and travels in the optical wave guide. The measured light beam which has been deflected is emitted from the optical waveguide and detected by a light detector. A modulator turns on and off or modulates the surface elastic wave by repeatedly energizing and de-energizing the surface elastic wave generator, the frequency of the modulation being lower than the continuously varying frequency. A filter receives a signal corresponding to the modulation frequency from the modulator and a signal corresponding to the measured light beam from the detector, and extracts a signal component having the same frequency as the modulation frequency from the signal received from the light detector. The frequency of the surface elastic wave is detected on the basis of the signal component extracted by the filler when the measured light beam is detected by the light detector.

Abstract translation: 光谱分析仪包括：表面弹性波发生器，用于在光波导中产生具有连续变化频率的表面弹性波，并且当导波在导波的路径上行进时衍射和偏转导波，该引导波被引入 作为测量光束进入并在光波导中行进。 已经偏转的测量光束从光波导发射并由光检测器检测。 调制器通过反复激励和断开表面弹性波发生器，使调制频率低于连续变化的频率来打开和关闭或调制表面弹性波。 滤波器从调制器接收对应于调制频率的信号和对应于来自检测器的测量光束的信号，并从从光检测器接收的信号中提取具有与调制频率相同频率的信号分量。 当由光检测器检测到测量光束时，基于由填料提取的信号分量来检测表面弹性波的频率。