Abstract:
An automatic taping lathe-microtome that produces a continuous ribbon of tissue by lathing an extremely thin strip off the surface of a cylindrical block containing a multitude of embedded tissue samples. Mechanisms are included for sandwiching this fragile ribbon of tissue between a pair of support tapes. Viewing holes are cut in the support tapes above and below each tissue slice such that the tapes act as slot grids allowing for direct viewing of each tissue slice in a transmission electron microscope (TEM). The resulting tissue-tape is placed on a spooling mechanism and fed into the beam of a TEM much like the film in a movie projector. This allows for random-access imaging of any section on the tape without requiring the TEM's vacuum be broken. This system is intended to give neuroscientists a tool to ultrastructure image large volumes of neural tissue and to trace multi-scale synaptic circuits.
Abstract:
PROBLEM TO BE SOLVED: To provide an improved method for extracting and handling multiple samples for S/TEM analysis.SOLUTION: Preferred embodiments of the present invention make use of a micromanipulator that attaches multiple samples at one time in a stacked formation and a method of placing each of the samples onto a TEM grid. By using a method that allows the processing of multiple samples, the throughput of sample preparation is increased significantly.
Abstract:
PROBLEM TO BE SOLVED: To provide a substrate processing apparatus capable of precisely performing temperature management and temperature control of the peripheral part and the central part of a substrate independently and simplifying a piping structure.SOLUTION: A substrate processing apparatus for processing substrates in a vacuum processing space includes a substrate mounting table for mounting at least two substrates thereon. The substrate mounting table includes substrate mounting units whose number corresponds to the number of the substrates mounted on the substrate mounting table. Each of the substrate mounting units includes a central temperature control flow path for cooling a central portion of the substrates to be mounted and a peripheral temperature control flow path for cooling a peripheral portion of the substrates, and the central temperature control flow path and the peripheral temperature control flow path are formed independently of each other. The substrate mounting table includes one temperature control medium inlet port for introducing a temperature control medium into the peripheral temperature control flow path, and temperature control medium outlet ports for discharging the temperature control medium from the peripheral temperature control flow path. The number of the temperature control medium outlet ports corresponds to the number of substrates to be mounted.
Abstract:
PROBLEM TO BE SOLVED: To solve a problem that, since an objective lens having the most important role in electromagnetic lenses used for an electron microscope achieves a short focal length by a large exciting current to perform high spatial resolution, on the other hand, in case if it is used for dimension instrumentation, reproducibility of image forming condition is insufficient due to magnetic hysteresis, as well as observation in a low magnification of about 200 to 2,000 times is difficult, and also, since the specimen is disposed in a magnetic field generated in the objective lens, the specimen is observed in a state of being always immersed in a magnetic field. SOLUTION: The electron beam observation device includes a mechanism which disposes a specimen at an upstream side in an electron beam traveling direction outside an objective lens, from which an image is transferred under a magnification of 1/5 to 1/30, in addition to an inside of the objective lens in which a specimen is disposed at a time of ordinary observation. COPYRIGHT: (C)2009,JPO&INPIT