公开(公告)号：WO2008076406A8

公开(公告)日：2008-09-12

申请号：PCT/US2007025721

申请日：2007-12-14

Applicant: ION TORRENT SYSTEMS INC ,  ROTHBERG JONATHAN M ,  HINZ WOLFGANG ,  JOHNSON KIM L ,  BUSTILLO JAMES

Inventor： ROTHBERG JONATHAN M ,  HINZ WOLFGANG ,  JOHNSON KIM L ,  BUSTILLO JAMES

IPC: G01N27/414 ,  G01N33/543

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/42324 ,  H01L29/78 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101

Abstract: A method for sequencing a nucleic acid may include disposing a plurality of template nucleic acids into a plurality of reaction chambers, wherein the plurality of reaction chambers is in contact with a chemical-sensitive field effect transistor (chemFET) array comprising at least one chemFET for each reaction chamber, wherein the at least one chemFET has a floating gate, and wherein each of the template nucleic acids is hybridized to a sequencing primer and is bound to a polymerase. The method may further include synthesizing a new nucleic acid strand by incorporating one or more known nucleotide triphosphates sequentially at the 3' end of the sequencing primer and detecting the incorporation of the one or more known nucleotide triphosphates by a change in current at the at least one chemFET within the array.

公开(公告)号：WO2007081385A3

公开(公告)日：2007-07-19

申请号：PCT/US2006/021280

申请日：2006-06-01

Applicant: RAINDANCE TECHNOLOGIES, INC. ,  LINK, Darren, R. ,  BOITARD, Laurent ,  BRANCIFORTE, Jeffrey ,  CHARLES, Yves ,  FEKE, Gilbert ,  LU, John, Q. ,  MARRAN, David ,  TABATABAI, Ahmadali ,  WEINER, Michael ,  HINZ, Wolfgang ,  ROTHBERG, Jonathan, M.

Inventor： LINK, Darren, R. ,  BOITARD, Laurent ,  BRANCIFORTE, Jeffrey ,  CHARLES, Yves ,  FEKE, Gilbert ,  LU, John, Q. ,  MARRAN, David ,  TABATABAI, Ahmadali ,  WEINER, Michael ,  HINZ, Wolfgang ,  ROTHBERG, Jonathan, M.

IPC: G01N33/536 ,  C12Q1/68 ,  B01J19/00 ,  C07B61/00 ,  C07K1/04 ,  G01N33/68

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.

公开(公告)号：WO2005049652A3

公开(公告)日：2006-01-12

申请号：PCT/US2004038488

申请日：2004-11-17

Applicant: ROTHBERG INST FOR CHILDHOOD DI ,  WEINER MICHAEL P ,  ROTHBERG JONATHAN M

Inventor： WEINER MICHAEL P ,  ROTHBERG JONATHAN M

IPC: C07K16/00 ,  C07K16/24 ,  C07K16/28 ,  C12N15/10 ,  C12Q1/68

CPC classification number: C07K16/2803 ,  C07K16/241 ,  C07K2317/34 ,  C07K2317/622

Abstract: The present invention provides a high throughput method for generating fully human monoclonal antibodies.

Abstract translation: 本发明提供了用于产生完全人单克隆抗体的高通量方法。

公开(公告)号：WO2004070007A2

公开(公告)日：2004-08-19

申请号：PCT/US2004/002571

申请日：2004-01-28

Applicant: 454 CORPORATION ,  COSTA, Gina, L. ,  WEINER, Michael, P. ,  LEAMON, John ,  ROTHBERG, Jonathan

Inventor： COSTA, Gina, L. ,  WEINER, Michael, P. ,  LEAMON, John ,  ROTHBERG, Jonathan

IPC: C12N

CPC classification number: C12Q1/686 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/5085 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0877 ,  C07H21/00 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q1/6867 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/253 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6484 ,  G01R33/1269 ,  Y02P20/582 ,  Y10T436/143333 ,  C12Q2565/119 ,  C12Q2531/113 ,  C12Q2565/537 ,  C12Q2547/107 ,  C12Q2547/101 ,  C12Q2537/143 ,  C12Q2527/125 ,  C12Q2525/186 ,  C12Q2565/301 ,  C12Q2563/149 ,  C12Q2531/125 ,  C12Q2537/149 ,  C12Q2535/119

Abstract: This invention relates to methods of generating single stranded DNA libraries for use in amplification and sequencing reactions. In various aspects, the disclosed methods include: fragmenting DNA; polishing the fragments' ends; lighting the fragments to universal adapters; performing strand displacement and extension of the nicked fragments; purifying the double-stranded libation products; capturing the double-stranded libation products onto a solid support; and isolating single stranded DNA library fragments, and binding these fragments to another solid support.

Abstract translation: 本发明涉及产生用于扩增和测序反应的单链DNA文库的方法。 在各方面，所公开的方法包括：片段化DNA; 抛光片段的末端; 将碎片照亮通用适配器; 进行缝合位移和切口碎片的延伸; 纯化双链奠基药品; 将双链奠基产品捕获到固体支持物上; 并分离单链DNA文库片段，并将这些片段结合到另一种固体支持物上。

公开(公告)号：WO2004070005A2

公开(公告)日：2004-08-19

申请号：PCT/US2004002485

申请日：2004-01-28

Applicant: 454 CORP ,  CHEN YI-JU ,  LEAMON JOHN H ,  LOHMAN KENTON ,  RONAN MICHAEL T ,  SRINIVASAN MAITHREYAN ,  ROTHBERG JONATHAN ,  WEINER MICHAEL

Inventor： CHEN YI-JU ,  LEAMON JOHN H ,  LOHMAN KENTON ,  RONAN MICHAEL T ,  SRINIVASAN MAITHREYAN ,  ROTHBERG JONATHAN ,  WEINER MICHAEL

IPC: B01L3/00 ,  C07H21/00 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68 ,  G01N21/25 ,  G01N21/64 ,  C12N

CPC classification number: C12Q1/686 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/5085 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0877 ,  C07H21/00 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q1/6867 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/253 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6484 ,  G01R33/1269 ,  Y02P20/582 ,  Y10T436/143333 ,  C12Q2565/119 ,  C12Q2531/113 ,  C12Q2565/537 ,  C12Q2547/107 ,  C12Q2547/101 ,  C12Q2537/143 ,  C12Q2527/125 ,  C12Q2525/186 ,  C12Q2565/301 ,  C12Q2563/149 ,  C12Q2531/125 ,  C12Q2537/149 ,  C12Q2535/119

Abstract: This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer to a first strand of nucleic acid; annealing a second blocked primer to a second strand of nucleic acid; elongating the nucleic acid along the first strand with a polymerase; terminating the first sequencing primer; deblocking the second primer; and elongating the nucleic acid along the second strand.

Abstract translation: 本发明涉及测序DNA的方法。 更具体地说，本发明涉及通过使用封闭和未封闭的测序引物对DNA的有义链和反义链测序的方法。 简言之，这些方法包括以下步骤：将未封闭的引物退火至第一链核酸; 将第二封闭引物退火至第二核酸链; 用聚合酶沿第一链延伸核酸; 终止第一测序引物; 解封第二引物; 并沿着第二链延长核酸。

公开(公告)号：WO2011106770A3

公开(公告)日：2012-04-05

申请号：PCT/US2011026468

申请日：2011-02-28

Applicant: LIFE TECHNOLOGIES CORP ,  DAVIDSON JOHN F ,  HINZ WOLFGANG ,  ROTHBERG JONATHAN ,  WHITAKER RICHARD

Inventor： DAVIDSON JOHN F ,  HINZ WOLFGANG ,  ROTHBERG JONATHAN ,  WHITAKER RICHARD

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/48

CPC classification number: C12N9/1252

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

公开(公告)号：WO2010008480A2

公开(公告)日：2010-01-21

申请号：PCT/US2009/003766

申请日：2009-06-25

Applicant: ION TORRENT SYSTEMS INCORPORATED ,  ROTHBERG, Jonathan, M. ,  HINZ, Wolfgang ,  JOHNSON, Kim, L. ,  BUSTILLO, James, M. ,  LEAMON, John, H. ,  SCHULTZ, Jonathan

Inventor： ROTHBERG, Jonathan, M. ,  HINZ, Wolfgang ,  JOHNSON, Kim, L. ,  BUSTILLO, James, M. ,  LEAMON, John, H. ,  SCHULTZ, Jonathan

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: C12Q1/6874 ,  B01L3/502761 ,  B01L2300/046 ,  B01L2300/0663 ,  B01L2300/0851 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N27/4148 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2565/629

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

Abstract translation: 与用于分析物测量的非常大规模的FET阵列相关的方法和装置。 可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET（例如，ISFET）阵列，并且同时促进显着小的像素尺寸和致密阵列。 改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。 在一个示例中，chemFET阵列基于监测无机焦磷酸盐（PPi），氢离子和核苷酸三磷酸盐的浓度变化来促进DNA测序技术。

公开(公告)号：WO2007081387A1

公开(公告)日：2007-07-19

申请号：PCT/US2006/021380

申请日：2006-06-01

Applicant: RAINDANCE TECHNOLOGIES, INC. ,  LINK, Darren, R. ,  BOITARD, Laurent ,  BRANCIFORTE, Jeffrey ,  CHARLES, Yves ,  FEKE, Gilbert ,  LU, John, Q. ,  MARRAN, David ,  TABATABAI, Ahmadali ,  WEINER, Michael ,  HINZ, Wolfgang ,  ROTHBERG, Jonathan, M.

Inventor： LINK, Darren, R. ,  BOITARD, Laurent ,  BRANCIFORTE, Jeffrey ,  CHARLES, Yves ,  FEKE, Gilbert ,  LU, John, Q. ,  MARRAN, David ,  TABATABAI, Ahmadali ,  WEINER, Michael ,  HINZ, Wolfgang ,  ROTHBERG, Jonathan, M.

IPC: G01N33/536 ,  C12Q1/68 ,  B01J19/00 ,  C07B61/00 ,  C07K1/04 ,  G01N33/68

CPC classification number: C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

Abstract: The present invention provides novel microfluidic devices, kits, and methods that are useful for performing high-throughput screening assays and diagnostics. Such diagnostic methods can include emulsifying an aqueous library of compounds with a set of uniquely dyecoded-labeled droplets in an inert fluorocarbon medium, thereby forming an interactor library, emulsifying an aqueous sample from a subject in an inert fluorocarbon medium, wherein said sample contains a compound that will react with at least one interactor molecule from the interactor library, coalescing the emulsions to form a nanoreactor, and screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, herein one or more steps are performed on a microfluidic device, which can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids.

Abstract translation: 本发明提供了用于进行高通量筛选测定和诊断的新型微流体装置，试剂盒和方法。 这样的诊断方法可以包括用惰性碳氟介质中的一组独特的经编码标记的液滴乳化化合物的水性文库，从而形成相互作用文库，在惰性碳氟介质中乳化来自受试者的含水样品，其中所述样品含有 将与来自相互作用文库的至少一个相互作用分子反应的化合物，聚合乳液以形成纳米反应器，并筛选纳米反应器以获得纳米反应器内容物之间所需的反应，这里，一个或多个步骤在微流体装置上进行， 其可以包括整体地布置在微加工基板上的多个可电寻址的通道支承流体模块，从而为不混溶流体的流动提供连续通道。

公开(公告)号：WO2007081386A2

公开(公告)日：2007-07-19

申请号：PCT/US2006021286

申请日：2006-06-01

Applicant: RAINDANCE TECHNOLOGIES INC ,  LINK DARREN R ,  BOITARD LAURENT ,  BRANCIFORTE JEFFREY ,  CHARLES YVES ,  FEKE GILBERT ,  LU JOHN Q ,  MARRAN DAVID ,  TABATABAI AHMADALI ,  WEINER MICHAEL ,  HINZ WOLFGANG ,  ROTHBERG JONATHAN M

Inventor： LINK DARREN R ,  BOITARD LAURENT ,  BRANCIFORTE JEFFREY ,  CHARLES YVES ,  FEKE GILBERT ,  LU JOHN Q ,  MARRAN DAVID ,  TABATABAI AHMADALI ,  WEINER MICHAEL ,  HINZ WOLFGANG ,  ROTHBERG JONATHAN M

IPC: B01L3/00 ,  B01F13/00 ,  G01N15/14

CPC classification number: C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The device can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids.

Abstract translation: 本发明提供了可用于进行高通量筛选测定和组合化学的新型微流体装置和方法。 该装置可以包括多个可电寻址的通道支承流体模块，其一体地布置在微加工基板上，使得提供用于不混溶流体流动的连续通道。

公开(公告)号：WO2007081385A2

公开(公告)日：2007-07-19

申请号：PCT/US2006021280

申请日：2006-06-01

Applicant: RAINDANCE TECHNOLOGIES INC ,  LINK DARREN R ,  BOITARD LAURENT ,  BRANCIFORTE JEFFREY ,  CHARLES YVES ,  FEKE GILBERT ,  LU JOHN Q ,  MARRAN DAVID ,  TABATABAI AHMADALI ,  WEINER MICHAEL ,  HINZ WOLFGANG ,  ROTHBERG JONATHAN M

Inventor： LINK DARREN R ,  BOITARD LAURENT ,  BRANCIFORTE JEFFREY ,  CHARLES YVES ,  FEKE GILBERT ,  LU JOHN Q ,  MARRAN DAVID ,  TABATABAI AHMADALI ,  WEINER MICHAEL ,  HINZ WOLFGANG ,  ROTHBERG JONATHAN M

IPC: G01N33/536 ,  B01J19/00 ,  C07B61/00 ,  C07K1/04 ,  C12Q1/68 ,  G01N33/68

CPC classification number: C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.

Abstract translation: 本发明提供了用于进行高通量筛选测定和组合化学的新型微流体装置和方法。 这样的方法可以包括通过乳化化合物的水溶液和独特液体标签的水溶液在微流体装置上标记化合物文库，所述微流体装置包括整体地布置在微制造基底上的多个可电寻址的流道承载流体模块， 通道用于不混溶流体的流动，由此每种化合物用独特的液体标签标记，汇集标记的乳液，使标记的乳液与含有特定细胞或酶的乳液聚结，由此形成纳米反应器，筛选纳米反应器以获得期望的反应 在纳米反应器的内容物之间，以及解码液体标签，从而从化合物文库中鉴定单一化合物。