-
公开(公告)号：KR1020090035372A
公开(公告)日：2009-04-09
申请号：KR1020070100626
申请日：2007-10-05
Applicant: 고려대학교 산학협력단
Abstract: A three-step differentiation protocol for generating spinal cord oligodendrocytes from human embryonic stem cells without foreign gene transfer is provided to prepare cell therapy compositions for treating spinal cord disease and spinal cord injury. A method for generating spinal cord oligodendrocytes from human embryonic stem cells comprises the following steps of: cultivating the human embryonic stem cells in a culture medium containing Sonic Hedgehog(Shh), Retinoic acid(RA) and noggin to prepare embryoid wherein ventral part spinal nerve ectoderm is increased in a first step; cultivating the embryoid in a culture medium containing basic fibroblast growth factor(bFGF) and platelet-derived growth factor(PDGF) in order to induce growth of precursor cell for the spinal cord oligodendrocytes in a second step; and cultivating the precursor cell in a culture medium containing cyclic adenosine monophosphate(cAMP), insulin-like growth factor 1(IGF-1) and neurotrophin 3(NT-3) to induce differentiation of the spinal cord oligodendrocytes in a third step.
Abstract translation: 提供用于从没有外源基因转移的人胚胎干细胞产生脊髓少突胶质细胞的三步分化方案，以制备用于治疗脊髓疾病和脊髓损伤的细胞治疗组合物。 从人胚胎干细胞产生脊髓少突胶质细胞的方法包括以下步骤：在含有Sonic Hedgehog（Shh），视黄酸（RA）和头蛋白的培养基中培养人胚胎干细胞，以制备其中腹侧脊神经 外胚层在第一步增加; 在含有碱性成纤维细胞生长因子（bFGF）和血小板衍生生长因子（PDGF）的培养基中培养胚胎，以在第二步中诱导脊髓少突胶质细胞前体细胞的生长; 并在第三步中培养含有环磷酸腺苷（cAMP），胰岛素样生长因子1（IGF-1）和神经营养因子3（NT-3）的培养基中诱导前体细胞，诱导脊髓少突胶质细胞的分化。
-
公开(公告)号：KR100885854B1
公开(公告)日：2009-02-26
申请号：KR1020060118102
申请日：2006-11-28
Applicant: 고려대학교 산학협력단
Abstract: 본 발명은 인간배아 줄기세포로부터 배아체가 형성되는 과정에서 혈청, 액티빈 (activin A) 및 레티노산 (retinoic acid)을 순차적으로 처리하여 췌장내배엽이 증가된 배아체를 유도하고, 이를 췌장으로 분화 가능한 췌장전구세포로 분화를 촉진시키는 방법에 관한 것이다.
본 발명에 따르면, 이미 어느 정도 분화가 진행된 성체줄기세포와는 달리 배아줄기세포는 여러 단계의 분화과정을 거쳐 기능성 체세포로 분화가 되며, 각 단계에서 원치 않는 세포로 분화될 가능성이 있으므로, 초기 내배엽으로의 효과적인 단계별 분화촉진 및 이에 뒤따르는 췌장세포로 분화시킬 수 있다. 또한, 배아줄기세포로부터 내배엽으로의 분화를 우선적으로 촉진시킨 뒤, 췌장세포로의 분화를 유도할 경우 기존의 분화유도방법보다 췌장세포로의 분화수율을 증가시켜 대량의 췌장베타세포를 획득할 수 있다.
인간배아 줄기세포, 췌장전구세포, 혈청, 액티빈, 레티노산, 췌장 내배엽, 배아체, 췌장베타세포-
公开(公告)号：KR1020080023079A
公开(公告)日：2008-03-12
申请号：KR1020060110630
申请日：2006-11-09
Applicant: 고려대학교 산학협력단
Abstract: A method for inducing differentiation of stem cells is provided to reduce the costs for differentiation by using inexpensive lithium chloride as a differentiation-inducing agent, induce the differentiation phenomenon similar as a normal development process in vitro culture, and increase differentiation efficiency. A method for inducing differentiation of stem cells comprises the steps of: (a) forming embryoid bodies from the stem cells through floating aggregation culture method; and (b) adding 5-20 mM of lithium chloride to the embryoid bodies and culturing them in a culture vessel containing an extracellular substrate such as laminin, collagen or Engelbreth-Holm-Swarm. Further, liver cell separating medicine is added into the embryoid bodies.
Abstract translation: 提供诱导干细胞分化的方法，通过使用廉价的氯化锂作为分化诱导剂来降低分化成本，诱导与体外培养中的正常发育过程相似的分化现象，并提高分化效率。 诱导干细胞分化的方法包括以下步骤：（a）通过浮游聚集培养方法从干细胞形成胚状体; 和（b）向胚状体中加入5-20mM的氯化锂，并在含有细胞外底物如层粘连蛋白，胶原或恩格列布 - 霍尔姆 - 布姆的培养容器中培养。 此外，将肝细胞分离药物加入到胚状体中。
-
公开(公告)号：KR1020080023078A
公开(公告)日：2008-03-12
申请号：KR1020060110269
申请日：2006-11-09
Applicant: 고려대학교 산학협력단
Abstract: Methods for inducing differentiation from human embryonic stem cells to dopaminergic neurons are provided to block possibility of differentiation into various cells including myocardial cells, muscle cells, liver cells and hematopoietic cells, so that differentiation efficiency into high purity nerve cells for cell therapy is increased. A method for inducing differentiation from human embryonic stem cells to dopaminergic neurons comprises the steps of: treating embryoid bodies formed from human embryonic stem cells with 40-60 ng/ml of a vascular endothelial growth factor(VEGF) for 7-9 days to induce the neuroectoderm-increased embryoid bodies; culturing the neuroectoderm-increased embryoid bodies attached to a tissue culture dish to induce neural precursor cells; detaching the induced neural precursor cells from the tissue culture dish and re-attaching and culturing them in the dish to increase purity and maintain density uniformly; and finally differentiating the high purity of neural precursor cells to dopaminergic neurons. Further, a 20-30 ug/ml of fibronectin is added into the neuroectoderm-increased embryoid bodies.
Abstract translation: 提供诱导从人胚胎干细胞分化为多巴胺能神经元的方法，以阻断分化成包括心肌细胞，肌细胞，肝细胞和造血细胞在内的各种细胞的可能性，从而增加细胞治疗的高纯度神经细胞的分化效率。 诱导从人胚胎干细胞分化为多巴胺能神经元的方法包括以下步骤：用40-60ng / ml血管内皮生长因子（VEGF）处理由人胚胎干细胞形成的胚状体7-9天以诱导 神经外胚层增生的胚状体; 培养附着于组织培养皿的神经外胚层增加的胚状体以诱导神经前体细胞; 从组织培养皿中分离诱导的神经前体细胞，并将其重新附着并培养在培养皿中以提高纯度并均匀地保持密度; 最后将神经前体细胞的高纯度区分为多巴胺能神经元。 此外，将20-30μg/ ml的纤连蛋白加入到神经外胚层增加的胚状体中。
-
公开(公告)号：KR1020170103697A
公开(公告)日：2017-09-13
申请号：KR1020170027813
申请日：2017-03-03
Applicant: 고려대학교 산학협력단
CPC classification number: A61K35/28 , A61K35/30 , A61K35/34 , A61K35/36 , A61K35/407 , A61K35/51 , A61K48/00
Abstract: 본발명은엑소좀또는엑소좀유래리보핵산을포함하는간섬유증예방또는치료용조성물또는세포치료제에관한것이다. 본발명의엑소좀및 이로부터유래된리보핵산은간성상세포및 쿠퍼세포의활성을억제하고α-SMA의발현을감소시키는효과가있으며, 교원질의침착을억제하여간섬유증의진행을억제시킴으로써간섬유증의예방이나치료에있어서세포치료제로유용하게이용될수 있다.
Abstract translation: 本发明涉及一种组合物或用于预防或治疗肝纤维化，包括外切位或位外 - 来源的RNA的细胞疗法。 通过外切从比特和由其本发明的衍生的核糖核酸肝纤维化抑制肝星状细胞和枯否氏细胞的活性，并具有减少α-SMA的表达，抑制胶原蛋白的沉积在任何速率，抑制纤维化的进展的效果 可以有效地用作预防或治疗癌症的细胞治疗剂。
-
Patent Agency Ranking
公开(公告)号：KR100941036B1
公开(公告)日：2010-02-05
申请号：KR1020070100626
申请日：2007-10-05
Applicant: 고려대학교 산학협력단
Abstract: 본 발명은 인간 배아줄기세포를 소닉 헷조그(Sonic Hedgehog, Shh), 레티놀 산(Retinoic acid, RA), 노긴(noggin) 및 비트로넥틴(vitronectin)을 포함하는 배지에서 배양하여 복측 척수신경계세포가 증가된 배상체를 유도하는 것을 특징으로 하는 인간 배아줄기세포로부터 척수 희소돌기 아교세포로의 분화유도 방법에 관한 것이다.
본 발명에 따르면, 인간배아줄기세포를 정상 발생과정과 매우 유사한 후성학적인 세포분화의 최적 환경을 단계별로 조성하여 외부 유전자의 인위적 도입과정 없이 인간배아 줄기세포로부터 희소돌기 아교세포를 효과적으로 분화유도 할 수 있다. 또한 상기 분화 유도된 희소돌기 아교세포 또는 그 전구세포를 이용하여 척수질환 및 척수 손상 치료용 세포치료제 조성물을 개발할 수 있다.-
27.发明公开나노 다이아몬드 입자의 정전하 자기조립 방법을 이용한기판의 전처리방법 및 이를 이용한 다이아몬드 박막증착방법 失效使用具有纳米金刚石颗粒的静电自组装工艺预处理脱模剂的方法和使用其的金刚石薄膜的沉积方法
公开(公告)号：KR1020080063227A
公开(公告)日：2008-07-03
申请号：KR1020070141892
申请日：2007-12-31
Applicant: 고려대학교 산학협력단
IPC: H01L21/208 , B82Y10/00
CPC classification number: C23C16/02 , B82Y40/00 , C23C16/27 , H01L21/02697
Abstract: A method for pre-treating a substrate using an electrostatic self-assembly process with nano diamond particles and a method for depositing a diamond thin film using the same. A surface of a nano diamond particle is coated with a polymer having an opposite polarity with respect to an electrostatic charge on the surface of the nano diamond particle. A surface of a substrate is treated with a polymer having an opposite polarity with respect to an electrostatic discharge on the substrate. The surface-treated substrate is immersed into a solution in which the coated nano diamond particles are dispersed. The nano diamond particle is deposited on the substrate through an electrostatic self-assembly process, so that it is used as a deposition core during a diamond thin film deposition. The polymer is selected from a group consisting of PSS(Poly Sodium 4-styrenen sulfonate), poly S-119, polyaniline, nafion, and PDDA(Poly DiallylDimethyl Ammonium chloride).
Abstract translation: 使用具有纳米金刚石颗粒的静电自组装工艺预处理衬底的方法和使用其沉积金刚石薄膜的方法。 纳米金刚石颗粒的表面涂覆有相对于纳米金刚石颗粒表面上的静电电荷具有相反极性的聚合物。 用相对于衬底上的静电放电具有相反极性的聚合物处理衬底的表面。 将表面处理的基材浸入其中分散有涂覆的纳米金刚石颗粒的溶液中。 纳米金刚石颗粒通过静电自组装工艺沉积在衬底上，使得其在金刚石薄膜沉积期间用作沉积芯。 聚合物选自PSS（聚4-苯乙烯磺酸钠），聚S-119，聚苯胺，nafion和PDDA（聚二烯丙基二甲基氯化铵）组成的组。
-
-
-
公开(公告)号：KR101971336B1
公开(公告)日：2019-04-22
申请号：KR1020170050882
申请日：2017-04-20
Applicant: 고려대학교 산학협력단
IPC: A61K47/50 , A61K31/437 , A61K49/00
-
-
-
-
-
-
-
-
-
Search Results
35
records
(took 0.024 seconds)
