Abstract:
PURPOSE: A method for transforming a plant using Agrobacteria is provided to remarkably improve transformation efficiency. CONSTITUTION: A method for trasforming a plant body using Agrobacteria comprises a step of removing albumen from the plant body and co-culturing the plant body with the Agrobacteria containing a recombinant expression vector for 7 or more days. The plant is cultured is cultured in an N6 meidum containing 1-3 mg/L of 2,4-dichlorophenoxyacetic acid at 25-32 Deg. C. for 4-7 days before removing albumen. The plant body is a callus of Oryza sativa. The Agrobacteria is Agrobacterium thumefacience strain LBA4404.
Abstract translation:目的:提供使用农杆菌转化植物的方法,以显着提高转化效率。 构成:使用农杆菌转化植物体的方法包括从植物体中除去白蛋白并与含有重组表达载体的农杆菌共培养植物体7天或更多天的步骤。 将培养的植物在含有1-3mg / L 2,4-二氯苯氧基乙酸的N6薄荷中以25-32度进行培养。 在清除白蛋白前4-7天。 植物体是水稻的愈伤组织。 农杆菌是土壤杆菌耐药菌株LBA4404。
Abstract:
본 발명은 벼 유래의 OsZIP1 단백질 및 이를 코딩하는 핵산 분자에 관한 것이다. 본 발명의 OsZIP1 핵산 분자를 이용하여 식물체를 형질전환시킴으로써, 병 저항성을 가지며, 야생형에 비하여 식물 개체의 크기 감소, 수술 길이의 단축, 꽃의 크기 축소 또는 화분 미형성의 생육 억제 양상 및 진한 화색의 표현형을 유도할 수 있으며, 이를 통해 식물(특히, 화훼작물)의 생산성 및 상품성(미니 꽃 생산)의 증대를 도모할 수 있다. 병 저항성, 벼, 담배, OsZIP1, 생육 억제
Abstract:
PURPOSE: An StMyb gene having salt-resistance or drought-resistance of plant is provided to use in developing plant species. CONSTITUTION: An StMyb(Solanum tuberosum Myb) polypeptide comprises an amino acid of sequence number 1. An StMyb polynucleotide has a gene sequence of sequence number 2. The intracellular increase of StMyb polypeptide is performed by transforming a plant with a vector containing the polynucleotide and a promoter. A method for producing the plant enhancing salt tolerance or drought tolerance comprises: a step of overexpressing StMyb polypeptide having amino acid of sequence number 1; and a step of transforming the plant with the expression vector.