公开(公告)号：KR101434996B1

公开(公告)日：2014-07-29

申请号：KR1020120140304

申请日：2012-12-05

Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 메디안디노스틱

Inventor： 송재영 ,  최은진 ,  이창희 ,  이진용 ,  이은정 ,  고희영 ,  정광면

IPC: G01N33/53 ,  G01N33/52 ,  G01N21/47 ,  G01N35/08

公开(公告)号：KR1020140072984A

公开(公告)日：2014-06-16

申请号：KR1020120140303

申请日：2012-12-05

Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 메디안디노스틱

Inventor： 송재영 ,  최은진 ,  이창희 ,  이진용 ,  김은주 ,  신연경 ,  이은정 ,  고희영 ,  박경민 ,  정광면

IPC: C12Q1/68 ,  G01N33/563 ,  G01N33/53

CPC classification number: C12Q1/701 ,  C12Q1/6888 ,  G01N33/5308 ,  G01N33/563

Abstract: The present invention provides a bio-probe for detecting novel influenza H1N1, comprising: (a) a nanomaterial; (b) an antibody which specifically binds to an antigen of novel influenza H1N1 which is covalently linked to the surface of the nanomaterial; and (c) a glass fiber as a solid substrate having a surface modified with the nanomaterial to which the antibody is conjugated. The present invention provides a method for diagnosing novel influenza H1N1 using the bio-probe. The present invention can promptly determine, within 10 minutes, the presence or not of novel influenza H1N1 and the infection or not with novel influenza H1N1 using a small amount of sample.

Abstract translation: 本发明提供了用于检测新型流感H1N1的生物探针，其包括：（a）纳米材料; （b）特异性结合与纳米材料的表面共价连接的新型流感H1N1的抗原的抗体; 和（c）作为固体基质的玻璃纤维，其具有用与抗体结合的纳米材料修饰的表面。 本发明提供使用生物探针诊断新型流感H1N1的方法。 本发明可以在10分钟内迅速确定新流行性感冒H1N1的存在与否，以及使用少量样品的新型H1N1感染。

公开(公告)号：KR1020130078057A

公开(公告)日：2013-07-10

申请号：KR1020110146777

申请日：2011-12-30

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 최은진 ,  송재영 ,  이창희 ,  김기성 ,  정호섭 ,  강도현

IPC: A61K9/127 ,  A61K47/06 ,  A61K47/36

CPC classification number: A61K47/06 ,  A61K9/127 ,  A61K47/24

Abstract: PURPOSE: A liposome containing phospholipid and an acetylene group is provided to manufacture a sensor with enhanced sensitivity and to sensitively detect a target material in a sample. CONSTITUTION: A liposome containing an acetylene group comprises lipid bilayer containing first and second lipid molecules. The first lipid molecule is a diacetylene molecule with an epoxy group at the end. The second lipid molecule is diacyl glycerol-3-phosphate molecule. The diacetylene molecule has a structure of formula I. The diacyl glycerol-3-phosphate molecule is 1,2-dimyristoyl-sn-glycero-3-phosphate (DMPA). A method for detecting a target material in a sample comprises the steps of: contacting a target material-binding material with the target material in the sample; detecting an optical signal; and identifying the target material from the optical signal.

Abstract translation: 目的：提供含有磷脂和乙炔基团的脂质体，以制造灵敏度更高的传感器，并灵敏地检测样品中的目标物质。 构成：含有乙炔基的脂质体包含含有第一和第二脂质分子的脂质双层。 第一种脂质分子是最后具有环氧基团的乙炔分子。 第二种脂质分子是二酰基甘油-3-磷酸分子。 二乙炔分子具有式I的结构。二酰基甘油-3-磷酸酯分子是1,2-二肉豆蔻酰-sn-甘油基-3-磷酸酯（DMPA）。 用于检测样品中的目标材料的方法包括以下步骤：使目标材料结合材料与样品中的目标材料接触; 检测光信号; 以及从光信号识别目标材料。

公开(公告)号：KR1020130049377A

公开(公告)日：2013-05-14

申请号：KR1020110114369

申请日：2011-11-04

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 현방훈 ,  이창희 ,  김재조 ,  임성인 ,  최은진 ,  한규하 ,  송재영 ,  박경호

IPC: A01K51/00 ,  A61L9/01 ,  A61L101/06

CPC classification number: A01K51/00 ,  A61L2/20 ,  A61L2202/11

Abstract: PURPOSE: A disinfecting apparatus of chlorine dioxide gas is provided to prevent or treat sacbrood which a honey bee viral transmittable disease. CONSTITUTION: A disinfecting apparatus of chlorine dioxide comprises: a holder(100); a print circuit substrate(200) which is mounted at the side of the holder and has a UV LED; a power supply unit(300) which applies power to the print circuit substrate; and a medicine receiving unit(400) which is mounted at the holder and contained stabilized chlorine dioxide. The stabilized chlorine dioxide is gel, liquid, or solid phase. The power supply unit has an AC to DC converter for applying a current power of 3-9 bolts to the print circuit substrate.

Abstract translation: 目的：提供二氧化氯气体的消毒装置，以防止或治疗蜜蜂病毒可传播疾病的囊袋。 构成：二氧化氯消毒装置包括：保持器（100）; 印刷电路基板（200），其安装在所述保持件的侧面并具有UV LED; 电源单元（300），其向所述打印电路基板施加电力; 和药物接收单元（400），其安装在保持器上并且包含稳定的二氧化氯。 稳定的二氧化氯是凝胶，液体或固相。 电源单元具有AC-DC转换器，用于将3-9个螺栓的当前功率施加到印刷电路基板。

公开(公告)号：KR1020110123918A

公开(公告)日：2011-11-16

申请号：KR1020100043406

申请日：2010-05-10

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 박최규 ,  김성희 ,  이경기 ,  송윤경 ,  이오수 ,  이창희

IPC: C12N5/10 ,  C12N15/867 ,  A61K39/12 ,  A61P31/12

Abstract: PURPOSE: A PAM-pCD163 cell line for isolating porcine reproductive and respiratory syndrome(PRRS) virus is provided to develop a vaccine and to prevent PRRS. CONSTITUTION: A PAM-pCD163 cell line is prepared by expressing pCD163 in PAM cells through a retrovirus prepared by transforming plasmid containing pCD163 genes into HEK293 cells. The plasmid is pFB-Zeo-pCD163. A method for preparing the cell line comprises: a step of performing transfection of VSV-G glycoprotein and MMLVgag/pol gene, and a plasmid containing pCD163 into HEK293 cells to prepare retrovirus; and a step of inoculating the retrovirus into the PAM cells.

Abstract translation: 目的：提供用于分离猪繁殖与呼吸综合征（PRRS）病毒的PAM-pCD163细胞系，用于开发疫苗和预防PRRS。 构成：通过将含有pCD163基因的质粒转化到HEK293细胞中制备的逆转录病毒，通过在PAM细胞中表达pCD163来制备PAM-pCD163细胞系。 质粒是pFB-Zeo-pCD163。 一种制备细胞系的方法包括：将VSV-G糖蛋白和MMLVgag / pol基因转染的步骤和含有pCD163的质粒转染HEK293细胞以制备逆转录病毒; 以及将逆转录病毒接种到PAM细胞中的步骤。

公开(公告)号：KR1020080114192A

公开(公告)日：2008-12-31

申请号：KR1020070063532

申请日：2007-06-27

Applicant: 주식회사 중앙백신연구소 ,  대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 주한수 ,  윤인중 ,  이경원 ,  강성호 ,  최환원 ,  유성식 ,  현방훈 ,  송재영 ,  조인수 ,  최은진 ,  윤소라 ,  김인중 ,  표현미 ,  이창희

IPC: A61K39/145 ,  A61K39/12 ,  A61P11/00

CPC classification number: A61K39/12 ,  A61K2039/552 ,  Y10S424/815 ,  Y10S424/825

Abstract: A method of manufacturing a vaccine composition containing antigen extracted from pig tissue infected with a complex respiratory disease is provided to prevent and treat a post weaning multisystemic wasting syndrome(PMWS) and a pig respiratory organ complex syndrome(PRDC). A method of manufacture the vaccine composition comprises steps of: i) removing pig tissue infected with a complex respiratory diseases; ii) extracting antigen by adding a solution comprising a glycine buffer solution and triton X-100 of 0.5% to the tissue; and iii) being inactivated by a formalin.

Abstract translation: 提供含有由感染复合呼吸系统疾病的猪组织提取的抗原的疫苗组合物的制造方法，以预防和治疗断乳多系统消耗综合征（PMWS）和猪呼吸器官综合征综合征（PRDC）。 制备疫苗组合物的方法包括以下步骤：i）去除感染复杂呼吸系统疾病的猪组织; ii）通过向组织中加入包含甘氨酸缓冲液和0.5％的triton X-100的溶液来提取抗原; 和iii）被福尔马林灭活。