公开(公告)号：KR1020050117686A

公开(公告)日：2005-12-15

申请号：KR1020040042854

申请日：2004-06-11

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 이희수 ,  임숙경 ,  조윤상 ,  주이석 ,  임춘태 ,  강현미 ,  김종만

IPC: G01N33/543

CPC classification number: G01N33/56916 ,  G01N33/581 ,  G01N2400/50

Abstract: 본 발명은 살모넬라 티피뮤리움(Salmonella typhimurium), 살모넬라 엠반다카(Salmonella mbandaca), 및 살모넬라 엔테라이티디스(Salmonella enteritidis)에서 추출한, 그룹특이성 및 민감성이 우수하고 항원성이 뛰어난 LPS(lipopolysaccaride)를 혼합하여 항원으로 사용하는 것을 특징으로 하는 동물의 살모넬라 감염항체 검사키트를 제공한다.

公开(公告)号：KR100508519B1

公开(公告)日：2005-09-28

申请号：KR1020030008750

申请日：2003-02-12

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 최강석 ,  나진주 ,  손현주 ,  고영준 ,  주이석

IPC: C07K7/08

Abstract: 본 발명은 우역바이러스 항체 검출용 키트의 제조 또는 우역에 감염된 동물의 생체내 면역수준 평가 등에 활용할 수 있는 항원 정보의 제공에 유용한, 우역바이러스 뉴클레오캡시드(nucleocapsid) 단백질의 아미노산 서열 중 우역바이러스 항체가 특이적으로 반응하는 아미노산 서열을 갖는 신규 합성 펩타이드에 관한 것이다.

公开(公告)号：KR1020040095824A

公开(公告)日：2004-11-16

申请号：KR1020030026809

申请日：2003-04-28

Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  프린스톤바이오메디테크코포레이션 ,  (주)피비엠이스트

Inventor： 조인수 ,  현방훈 ,  이광녕 ,  엄재구 ,  계수정 ,  고영준 ,  구복경 ,  주이석 ,  안수환 ,  김인중 ,  김옥경 ,  김희정 ,  장기용 ,  신남규 ,  황서하 ,  강제모 ,  김창호 ,  고송우

IPC: G01N33/543

CPC classification number: G01N33/56983 ,  G01N33/526 ,  G01N33/558 ,  G01N2333/09 ,  Y10S436/811

Abstract: PURPOSE: A method for diagnosis of foot and mouth disease and a diagnostic kit are provided, thereby rapidly and accurately diagnosing foot and mouth disease with a small amount of a sample, and easily and rapidly diagnosing foot and mouth disease of domestic animals in the field. CONSTITUTION: The method for diagnosis of foot and mouth disease comprises the steps of: (1) introducing a testing sample into a loading area of a strip; (2) combining a detecting reagent with a marker with a target material in the sample to prepare a complex; (3) developing the complex on a membrane; and (4) measuring the appearance change of a reaction portion having one or more fixing phase selected from antigen, antibody or hapten which is obtainable through the immune reaction from FMDV in a certain region of the membrane. The diagnostic kit comprises a reaction portion(13) having one or more fixing phase selected from antigen, antibody or hapten which is obtainable through the immune reaction from FMDV; a strip(1') having a control portion for determining normal operation on a certain region; a testing sample introducing portion; and a housing with a displaying window(4) for displaying reaction results in the reaction portion(13) and the control portion. on the strip(1').

Abstract translation: 目的：提供口蹄疫诊断方法和诊断试剂盒，用少量样品快速，准确地诊断口蹄疫，容易快速诊断现场家畜的口蹄疫 。 规定：口蹄疫诊断方法包括以下步骤：（1）将检测样品引入条带的装载区域; （2）将检测试剂与标记物与样品中的靶材料组合以制备复合物; （3）在膜上显影复合物; 和（4）测量具有一个或多个定影相的反应部分的外观变化，所述固定相选自抗原，抗体或半抗原，所述抗原，抗体或半抗原可通过膜的某一区域中的FMDV通过免疫反应获得。 诊断试剂盒包括具有选自抗原，抗体或半抗原的一个或多个固定相的反应部分（13），其可通过FMDV的免疫反应获得; 具有用于确定某一区域正常操作的控制部分的条带（1'）; 测试样品引入部分; 以及具有用于在反应部（13）和控制部显示反应结果的显示窗（4）的壳体。 在条带（1'）上。

公开(公告)号：KR1020030052688A

公开(公告)日：2003-06-27

申请号：KR1020010082715

申请日：2001-12-21

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 권창희 ,  권병준 ,  고영준 ,  이세영 ,  주이석

IPC: G01N33/68

Abstract: PURPOSE: A VP7 antigen of African horse sickness virus(AHSV) and a diagnostic method of African horse sickness(AHS) using a monoclonal antibody therefor are provided, thereby rapidly and accurately diagnosing AHSV. CONSTITUTION: A diagnostic method of African horse sickness(AHS) using a monoclonal antibody to VP7 antigen of African horse sickness virus(AHSV) comprises the steps of: (1) adhering a monoclonal antibody for VP7 antigen of African horse sickness virus(AHSV) to a solid support; (2) binding the VP7 antigen of AHSV with the monoclonal antibody adhered to the solid support; (3) reacting a sample containing an antibody for a VP7 protein with the VP7 antigen of AHSV; (4) reacting a secondary antibody selected from the group consisting of an enzyme conjugated antibody, a radioactive material conjugated antibody and a fluorescent material conjugated antibody, with the antibody of the step 3; and (5) quantifying the antibody in the sample, wherein the monoclonal antibody for VP7 antigen of African horse sickness virus(AHSV) is produced from a hybridoma cell(KCTC 10136BP).

Abstract translation: 目的：提供非洲马病病毒（AHSV）的VP7抗原和使用其单克隆抗体的非洲马病诊断方法（AHS），从而快速准确地诊断AHSV。 构成：使用非洲马病病毒（AHSV）的VP7抗原单克隆抗体的非洲马病诊断方法（AHS）包括以下步骤：（1）将非洲马病病毒（AHSV）VP7抗原的单克隆抗体 坚定的支持; （2）将AHSV的VP7抗原与附着在固体支持物上的单克隆抗体结合; （3）将含有VP7蛋白抗体的样品与AHSV的VP7抗原反应; （4）将选自酶结合抗体，放射性物质缀合抗体和荧光物质缀合抗体的二抗与步骤3的抗体反应; 和（5）定量样品中的抗体，其中非洲马病病毒（AHSV）的VP7抗原单克隆抗体由杂交瘤细胞（KCTC 10136BP）产生。