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    면역 크로마토그래피법을 이용한 돼지콜레라바이러스항체의 검출 키트
    22.
    发明公开
    면역 크로마토그래피법을 이용한 돼지콜레라바이러스항체의 검출 키트 有权
    使用免疫印迹法检测猪流感病毒抗体的检测工具

    公开(公告)号：KR1020040051199A

    公开(公告)日：2004-06-18

    申请号：KR1020020079085

    申请日：2002-12-12

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 송재영 ,  최은진 ,  박종현 ,  안동준 ,  차상호 ,  권준헌

    IPC: G01N33/543

    Abstract: PURPOSE: A detection kit for swine cholera virus antibody using immunochromatography method is provided, thereby rapidly detecting the swine cholera virus antibody, and enabling the in-situ detection of the swine cholera virus antibody, so that the swine cholera virus antibody can be rapidly detected in farmhouses. CONSTITUTION: The detection kit for swine cholera virus antibody using immunochromatography method is prepared by binding a protein antigen(1), preferably E2 protein of swine cholera virus to strip type nitrocellulose membrane(5); and binding anti-swine IgG antibody(2) to the opposite side of the nitrocellulose membrane(5). The method for detecting the swine cholera virus antibody comprises the steps of: contacting a swine blood sample with the nitrocellulose membrane(5) by spotting the sample on the sample pad through a sample loading hole(4); transferring the swine blood sample to the swine cholera virus antigen(1) via the nitrocellulose membrane(5), and transferring the anti-swine IgG gold conjugate adsorbed in a conjugate pad to the absorbing pad(3); and measuring the occurrence of red purple by the antigen-antibody reaction followed by the gold particle reaction with the antigen-antibody conjugate in the antigen fixed region(1).

    Abstract translation: 目的：提供使用免疫色谱法的猪霍乱病毒抗体检测试剂盒，从而快速检测猪霍乱病毒抗体，并能够对猪霍乱病毒抗体进行原位检测，从而快速检测猪霍乱病毒抗体 在农舍 构成：使用免疫色谱法测定猪霍乱病毒抗体检测试剂盒是通过将蛋白质抗原（1），优选鸡蛋霍夫病毒E2蛋白与带状硝酸纤维素膜结合而制备的（5）; 并将抗猪IgG抗体（2）与硝酸纤维素膜（5）的相反侧结合。 检测猪霍乱病毒抗体的方法包括以下步骤：通过样品装载孔（4）将样品垫上的样品点样，使猪血样与硝酸纤维素膜接触（5）; 通过硝酸纤维素膜（5）将猪血液样品转移到猪霍乱病毒抗原（1），并将吸附在共轭垫中的抗猪IgG金缀合物转移到吸收垫（3）上; 并通过抗原 - 抗体反应随后与抗原固定区域（1）中的抗原 - 抗体缀合物进行金颗粒反应来测量红紫色的发生。

    일본 뇌염 바이러스 유전자 엔에스1을 발현하는 돼지 아데노바이러스 3형
    23.
    发明公开
    일본 뇌염 바이러스 유전자 엔에스1을 발현하는 돼지 아데노바이러스 3형 有权
    重组蛋氨酸腺病毒3型表达日本ENCEPHALITIS病毒基因NS1及其用途

    公开(公告)号：KR1020040047484A

    公开(公告)日：2004-06-05

    申请号：KR1020020075721

    申请日：2002-11-30

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 차상호 ,  박종현 ,  송재영 ,  안동준 ,  최은진 ,  김종염 ,  권준헌

    IPC: C12N15/861

    CPC classification number: C12N15/861 ,  C07K14/005 ,  C12N2770/24034

    Abstract: PURPOSE: Recombinant porcine adenovirus type 3 expressing Japanese encephalitis virus gene NS1 and use thereof are provided, thereby inducing immunity to Japanese encephalitis virus in human, and improving stability of the vaccine by using adenovirus. CONSTITUTION: A recombinant vector of porcine adenovirus type 3 expressing Japanese encephalitis virus gene NS1, pPAV3E3-NS1(KCTC 10377BP), is prepared by removing E3 gene of porcine adenovirus type 3, and inserting the Japanese encephalitis virus gene NS1 under the control of E3 promoter, wherein the Japanese encephalitis virus gene NS1 has the nucleotide sequence set forth in SEQ ID NO: 1. A host cell transformed with the recombinant vector pPAV3E3-NS1(KCTC 10377BP) is provided. The recombinant porcine adenovirus type 3 expressing Japanese encephalitis virus gene NS1(PAV3E3-NS1) is obtained by culturing the transformed host cell. A vaccine for Japanese encephalitis virus comprises the recombinant porcine adenovirus type 3 expressing Japanese encephalitis virus gene NS1.

    Abstract translation: 目的：提供3型表达日本脑炎病毒基因NS1的重组猪腺病毒及其用途，从而诱导人体对日本脑炎病毒的免疫，并通过使用腺病毒提高疫苗的稳定性。 构成：通过除去3型猪腺病毒的E3基因，并将日本脑炎病毒基因NS1插入到E3的控制下，制备3型表达日本脑炎病毒基因NS1，pPAV3E3-NS1（KCTC 10377BP）的猪腺病毒重组载体 启动子，其中日本脑炎病毒基因NS1具有SEQ ID NO：1所示的核苷酸序列。提供用重组载体pPAV3E3-NS1（KCTC 10377BP）转化的宿主细胞。 通过培养转化的宿主细胞获得3型表达日本脑炎病毒基因NS1（PAV3E3-NS1）的猪腺病毒。 日本脑炎病毒疫苗包含3型表达日本脑炎病毒基因NS1的重组猪腺病毒。

    인지질 및 아세틸렌기를 포함하는 리포좀 및 그의 용도
    29.
    发明公开
    인지질 및 아세틸렌기를 포함하는 리포좀 및 그의 용도 有权
    具有乙酰基环糊精和磷脂的脂质体及其用途

    公开(公告)号：KR1020130078057A

    公开(公告)日：2013-07-10

    申请号：KR1020110146777

    申请日：2011-12-30

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 최은진 ,  송재영 ,  이창희 ,  김기성 ,  정호섭 ,  강도현

    IPC: A61K9/127 ,  A61K47/06 ,  A61K47/36

    CPC classification number: A61K47/06 ,  A61K9/127 ,  A61K47/24

    Abstract: PURPOSE: A liposome containing phospholipid and an acetylene group is provided to manufacture a sensor with enhanced sensitivity and to sensitively detect a target material in a sample. CONSTITUTION: A liposome containing an acetylene group comprises lipid bilayer containing first and second lipid molecules. The first lipid molecule is a diacetylene molecule with an epoxy group at the end. The second lipid molecule is diacyl glycerol-3-phosphate molecule. The diacetylene molecule has a structure of formula I. The diacyl glycerol-3-phosphate molecule is 1,2-dimyristoyl-sn-glycero-3-phosphate (DMPA). A method for detecting a target material in a sample comprises the steps of: contacting a target material-binding material with the target material in the sample; detecting an optical signal; and identifying the target material from the optical signal.

    Abstract translation: 目的：提供含有磷脂和乙炔基团的脂质体，以制造灵敏度更高的传感器，并灵敏地检测样品中的目标物质。 构成：含有乙炔基的脂质体包含含有第一和第二脂质分子的脂质双层。 第一种脂质分子是最后具有环氧基团的乙炔分子。 第二种脂质分子是二酰基甘油-3-磷酸分子。 二乙炔分子具有式I的结构。二酰基甘油-3-磷酸酯分子是1,2-二肉豆蔻酰-sn-甘油基-3-磷酸酯（DMPA）。 用于检测样品中的目标材料的方法包括以下步骤：使目标材料结合材料与样品中的目标材料接触; 检测光信号; 以及从光信号识别目标材料。

    낭충봉아부패병을 포함한 꿀벌의 전염성 질병의 예방 및 치료를 위한 이산화염소 기체 소독장치
    30.
    发明公开
    낭충봉아부패병을 포함한 꿀벌의 전염성 질병의 예방 및 치료를 위한 이산화염소 기체 소독장치 有权
    生产二氧化氯气体的方法和方法，用于预防和治疗含有沙门氏菌的蜂蜜颗粒病

    公开(公告)号：KR1020130049377A

    公开(公告)日：2013-05-14

    申请号：KR1020110114369

    申请日：2011-11-04

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 현방훈 ,  이창희 ,  김재조 ,  임성인 ,  최은진 ,  한규하 ,  송재영 ,  박경호

    IPC: A01K51/00 ,  A61L9/01 ,  A61L101/06

    CPC classification number: A01K51/00 ,  A61L2/20 ,  A61L2202/11

    Abstract: PURPOSE: A disinfecting apparatus of chlorine dioxide gas is provided to prevent or treat sacbrood which a honey bee viral transmittable disease. CONSTITUTION: A disinfecting apparatus of chlorine dioxide comprises: a holder(100); a print circuit substrate(200) which is mounted at the side of the holder and has a UV LED; a power supply unit(300) which applies power to the print circuit substrate; and a medicine receiving unit(400) which is mounted at the holder and contained stabilized chlorine dioxide. The stabilized chlorine dioxide is gel, liquid, or solid phase. The power supply unit has an AC to DC converter for applying a current power of 3-9 bolts to the print circuit substrate.

    Abstract translation: 目的：提供二氧化氯气体的消毒装置，以防止或治疗蜜蜂病毒可传播疾病的囊袋。 构成：二氧化氯消毒装置包括：保持器（100）; 印刷电路基板（200），其安装在所述保持件的侧面并具有UV LED; 电源单元（300），其向所述打印电路基板施加电力; 和药物接收单元（400），其安装在保持器上并且包含稳定的二氧化氯。 稳定的二氧化氯是凝胶，液体或固相。 电源单元具有AC-DC转换器，用于将3-9个螺栓的当前功率施加到印刷电路基板。

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