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公开(公告)号：KR1020100082975A
公开(公告)日：2010-07-21
申请号：KR1020090002301
申请日：2009-01-12
Applicant: 대한민국(농촌진흥청장) , 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: C12N15/10 , C12N15/11 , C12Q1/6851 , C12Q1/6853 , C12Q1/701
Abstract: PURPOSE: A primer set for diagnosing tobacco rattle virus(TRV) and a method for diagnosing TRV using the same are provided to accurately diagnose TRV infection from Solanaceae seed or plant. CONSTITUTION: A primer set for diagnosing TRV comprises a forward primer selected from sequence numbers 1, 2, 3, 4, 5 and 6, and reverse primer selected from sequence numbers 9, 10, 11, 12, 13, 14 and 15. The primer set for diagnosing TRV comprises a primer set of sequence number 3 and 1 and primer set of sequence numbers 1 and 14. A method for diagnosing TRV comprises: a step of isolating total RNA from a plant sample; a step of amplifying a target sequence by RT-PCR; and a step of detecting PCR product.
Abstract translation: 目的：提供用于诊断烟草拨鼠病毒（TRV）的引物组和使用其的诊断TRV的方法，以准确诊断茄科种子或植物的TRV感染。 构成：用于诊断TRV的引物组包含选自序列号1,2,3,4,5和6的正向引物，以及选自序列号9,10,11,12,13,14和15的反向引物。 用于诊断TRV的引物组包含序列号3和1的引物组和序列号1和14的引物组。诊断TRV的方法包括：从植物样品中分离总RNA的步骤; 通过RT-PCR扩增靶序列的步骤; 以及检测PCR产物的步骤。
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公开(公告)号：KR1020100082973A
公开(公告)日：2010-07-21
申请号：KR1020090002299
申请日：2009-01-12
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/10 , C12N15/11 , C12Q1/6851 , C12Q1/6853 , C12Q1/701
Abstract: PURPOSE: A primer set for diagnosing arabis mosaic virus(ArMV) and a diagnosis method are provided to accurately diagnose ArMV infection of various plant and to minimize damage by ArMV. CONSTITUTION: A primer set for diagnosing ArMV comprises a forward primer and reverse primer. The forward primer selected from sequence numbers 1, 2, 3 and 4. The reverse primer is selected from sequence numbers 5, 7, and 8. A kit for diagnosing ArMV comprises the primer set and DNA fragment of sequence number 11.
Abstract translation: 目的：提供用于诊断阿拉伯花叶病毒（ArMV）的引物组和诊断方法，以准确诊断各种植物的ArMV感染，并尽量减少ArMV的损伤。 构成：用于诊断ArMV的引物组包括正向引物和反向引物。 正向引物选自序列号1,2,3和4.反向引物选自序列号5,7和8.用于诊断ArMV的试剂盒包含引物组和序列号11的DNA片段。
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公开(公告)号：KR1020100082972A
公开(公告)日：2010-07-21
申请号：KR1020090002298
申请日：2009-01-12
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/10 , C12N15/11 , C12Q1/6851 , C12Q1/6853 , C12Q1/701
Abstract: PURPOSE: A primer set for diagnosing TBSV and PCR test system for diagnosing TBSV using the same are provided to use for testing TBSV from a plant. CONSTITUTION: A primer set for diagnosing tomato bushy stunt virus(TBSV) comprises a forward primer and reverse primer. The forward primer is selected from sequence numbers 2, 3, 4, 5 and 6. The reverse primer is selected from sequence number 8, 9, 10, 11, 12 and 13. The primer set for diagnosing TBSV is a primer set of sequence numbers 3 and 10 and sequence numbers 3 and 11. A method for diagnosing TBSV comprises: a step of isolating RNA from a plant sample; a step of amplifying by RT-PCR; and a step of detecting PCR product.
Abstract translation: 目的：用于诊断TBSV的引物组和用于诊断TBSV的PCR检测系统，用于从工厂测试TBSV。 构成：用于诊断番茄丛生特技病毒（TBSV）的引物组包括正向引物和反向引物。 正向引物选自序列号2,3,4,5和6.反向引物选自序列号8,9,10,11,12和13.用于诊断TBSV的引物组是序列的引物组 数字3和10以及序列号3和11.用于诊断TBSV的方法包括：从植物样品中分离RNA的步骤; 通过RT-PCR扩增的步骤; 以及检测PCR产物的步骤。
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公开(公告)号：KR1020100082964A
公开(公告)日：2010-07-21
申请号：KR1020090002286
申请日：2009-01-12
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12N15/10 , C12Q1/6851 , C12Q1/6853 , C12Q1/701
Abstract: PURPOSE: A primer set for diagnosing cherry leaf roll virus(CLRV) and a method for diagnosing CLRV using the same are provided to test CVRV from a plant in immigration quarantine and clinical diagnosis. CONSTITUTION: A primer set for diagnosing CLRV comprises a forward primer and reverse primer. The forward primer is selected from sequence numbers 1, 2, 5, 6, 7, 8, 9 and 10. The reverse primer is selected from sequence numbers 14, 15, 16, 17, 18, 19, 20, 21 and 22. A kit for diagnosing CLRV comprises the primer set and DNA fragment having a base sequence of sequence number 24. A method for diagnosing CLRV comprises: a step of isolating total RNA from a plant sample; a step of amplifying a target sequence by RT-PCR; and a step of detecting PCR product. The plant is cherry, berries, walnut, bean, plum, or pea.
Abstract translation: 目的：提供用于诊断樱桃叶卷病毒（CLRV）的引物组和使用其的CLRV诊断方法，用于在植物检疫和临床诊断中检测CVRV。 构成：用于诊断CLRV的引物组包括正向引物和反向引物。 正向引物选自序列号1，2，5，6，7，8，9和10.反向引物选自序列号14,15,16,17,18,19,20,21和22。 用于诊断CLRV的试剂盒包括引物组和具有序列号24的碱基序列的DNA片段。用于诊断CLRV的方法包括：从植物样品中分离总RNA的步骤; 通过RT-PCR扩增靶序列的步骤; 以及检测PCR产物的步骤。 植物是樱桃，浆果，核桃，豆，李子或豌豆。
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公开(公告)号：KR1020100039934A
公开(公告)日：2010-04-19
申请号：KR1020080098925
申请日：2008-10-09
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/6827 , C12Q2600/112
Abstract: PURPOSE: A specific primer for diagnosing onion yellow dwarf virus(OYDV) is provided to simply and economically diagnose OYDV and produce normal garlic. CONSTITUTION: A primer set for diagnosing onion yellow dwarf virus(OYDV) comprise a forward primer of sequence number 1, 3 or 4 and reverse primer of sequence number 2 or 5. A primer set for diagnosing OYDV comprises a primer set of sequence numbers 1 and 2, primer set of sequence numbers 3 and 2, or primer set of sequence numbers 4 and 5. A method for OYDV-specific diagnosis comprises: a step of isolating total RNA from a garlic sample; a step of amplifying target sequence through RT-PCR using the primer set and RNA template; and a step of detecting the amplified product.
Abstract translation: 目的：用于诊断洋葱黄矮病毒（OYDV）的特异性引物简单经济地诊断OYDV并产生正常大蒜。 构成：用于诊断洋葱黄矮病毒（OYDV）的引物组包括序列号1,3或4的正向引物和序列号为2或5的反向引物。用于诊断OYDV的引物组包含序列号1的引物组 2，序列号3和2的引物组或序列号4和5的引物组。一种用于OYDV特异性诊断的方法包括：从大蒜样品中分离总RNA的步骤; 使用引物组和RNA模板通过RT-PCR扩增靶序列的步骤; 以及检测扩增产物的步骤。
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Patent Agency Ranking
公开(公告)号：KR1020100039932A
公开(公告)日：2010-04-19
申请号：KR1020080098923
申请日：2008-10-09
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q2600/112
Abstract: PURPOSE: A specific primer for diagnosing leek yellow stripe virus(LYSV) and a method for diagnosing are provided to overcome detection limit and use for producing high quality of garlic. CONSTITUTION: A primer set for diagnosing leek yellow stripe virus(LYSV) comprises a forward primer of sequence number 1 or 3 and reverse primer of sequence number 2 or 4. A primer set for LYSV diagnosis is primer set of sequence numbers 1 and 2 or primer set for sequence numbers 3 and 4. A kit for diagnosing LYSVA contains the primer set and reagent for RT-PCR. A method for diagnosing LYSV comprises: a step of isolating total RNA from a garlic sample; a step of amplifying target sequence by RT-PCR using the primer set and total RNA as a template; and a step of detecting amplified product.
Abstract translation: 目的：提供用于诊断韭黄色条纹病毒（LYSV）的特异性引物和诊断方法，以克服检测限，并用于生产高质量的大蒜。 构成：用于诊断韭黄条纹病毒（LYSV）的引物组包括序列号1或3的正向引物和序列号为2或4的反向引物.LYSV诊断的引物组是序列号1和2的引物组或 用于序列号3和4的引物组。用于诊断LYSVA的试剂盒含有用于RT-PCR的引物组和试剂。 用于诊断LYSV的方法包括：从大蒜样品中分离总RNA的步骤; 使用引物组和总RNA作为模板通过RT-PCR扩增靶序列的步骤; 以及检测扩增产物的步骤。
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公开(公告)号：KR101650551B1
公开(公告)日：2016-08-24
申请号：KR1020110117664
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
Abstract: 본발명은서열번호 1 내지 12의올리고뉴클레오티드및 이의상보적올리고뉴클레오티드로구성되는군으로부터선택되는하나이상의올리고뉴클레오티드를포함하는 AMV (Alfalfa mosaic virus) 진단또는검출용올리고뉴클레오티드프로브세트, 상기올리고뉴클레오티드프로브세트를포함하는 AMV 진단또는검출용유전자칩, AMV에감염된것으로추정되는시료로부터 RNA를분리하는단계, 상기분리된 RNA를주형으로하여 cDNA를합성하는단계, 상기합성된 cDNA를상기올리고뉴클레오티드프로브세트와접촉시켜상기 cDNA와프로브세트를혼성화시키는단계및 상기프로브와 cDNA 사이의혼성화정도를검출하는단계를포함하는 AMV를진단또는검출하는방법및 상기올리고뉴클레오티드프로브세트및 혼성화용액을포함하는 AMV 또는검출용키트에관한것이다.
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公开(公告)号：KR101650550B1
公开(公告)日：2016-08-24
申请号：KR1020110117658
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
Abstract: 본발명은서열번호 2122 내지 2125 및서열번호 2131의올리고뉴클레오티드및 이의상보적올리고뉴클레오티드로구성되는군으로부터선택되는하나이상의올리고뉴클레오티드를포함하는 PSTVd (Potato spindle tuber viroid) 진단또는검출용올리고뉴클레오티드프로브세트, 상기올리고뉴클레오티드프로브세트를포함하는 PSTVd 진단또는검출용유전자칩, PSTVd에감염된것으로추정되는시료로부터 RNA를분리하는단계, 상기분리된 RNA를주형으로하여 cDNA를합성하는단계, 상기합성된 cDNA를상기올리고뉴클레오티드프로브세트와접촉시켜상기 cDNA와프로브세트를혼성화시키는단계및 상기프로브와 cDNA 사이의혼성화정도를검출하는단계를포함하는 PSTVd를진단또는검출하는방법및 상기올리고뉴클레오티드프로브세트및 혼성화용액을포함하는 PSTVd 또는검출용키트에관한것이다.
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公开(公告)号：KR101650547B1
公开(公告)日：2016-08-24
申请号：KR1020110117624
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
Abstract: 본발명은서열번호 894 내지 909의올리고뉴클레오티드및 이의상보적올리고뉴클레오티드로구성되는군으로부터선택되는하나이상의올리고뉴클레오티드를포함하는 SqMV (Squash mosaic virus) 진단또는검출용올리고뉴클레오티드프로브세트, 상기올리고뉴클레오티드프로브세트를포함하는 SqMV 진단또는검출용유전자칩, SqMV에감염된것으로추정되는시료로부터 RNA를분리하는단계, 상기분리된 RNA를주형으로하여 cDNA를합성하는단계, 상기합성된 cDNA를상기올리고뉴클레오티드프로브세트와접촉시켜상기 cDNA와프로브세트를혼성화시키는단계및 상기프로브와 cDNA 사이의혼성화정도를검출하는단계를포함하는 SqMV를진단또는검출하는방법및 상기올리고뉴클레오티드프로브세트및 혼성화용액을포함하는 SqMV 진단또는검출용키트에관한것이다.
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公开(公告)号：KR101407237B1
公开(公告)日：2014-07-01
申请号：KR1020110117611
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
Abstract: 본 발명은 카모바이러스 속 (Genus Carmovirus) 바이러스에 특이적인 41개의 올리고뉴클레오티드 및 이의 상보적 올리고뉴클레오티드로 구성되는 군으로부터 선택되는 하나 이상의 올리고뉴클레오티드를 포함하는 카모바이러스 속 바이러스 진단 또는 검출용 올리고뉴클레오티드 프로브 세트, 상기 올리고뉴클레오티드 프로브 세트를 포함하는 카모바이러스 속 바이러스 진단 또는 검출용 유전자 칩, 카모바이러스 속 바이러스에 감염된 것으로 추정되는 시료로부터 RNA를 분리하는 단계, 상기 분리된 RNA를 주형으로 하여 cDNA를 합성하는 단계, 상기 합성된 cDNA를 상기 올리고뉴클레오티드 프로브 세트와 접촉시켜 상기 cDNA와 프로브 세트를 혼성화시키는 단계 및 상기 프로브와 cDNA 사이의 혼성화 정도를 검출하는 단계를 포함하는 카모바이러스 속 바이러스를 진단 또는 검출하는 방법 및 상기 올리고뉴클레오티드 프로브 세트 및 혼성화 용액을 포함하는 카모바이러스 속 바이러스는 검출용 키트에 관한 것이다.
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