국내 고구마 잎말림병 감염 DNA 바이러스의 양방향성프로모터
    22.
    发明公开
    국내 고구마 잎말림병 감염 DNA 바이러스의 양방향성프로모터 有权
    使用甜菜的双向促销蛋白

    公开(公告)号:KR1020090112492A

    公开(公告)日:2009-10-28

    申请号:KR1020080038415

    申请日:2008-04-24

    Abstract: PURPOSE: A bidirectional promoter of sweet potato leaf curl infection DNA virus is provided to produce vector product in molecular biology field and molecular breeding industry. CONSTITUTION: A bidirectional promoter of sweet potato leaf curl infection DNA virus is denoted by the sequence of the sequence number 1. A method for identifying the promoter activity comprises: a step of isolating a site with promoter activity and analyzing cis-acting regulation site; a step of recombining promoter site by using gateway cloning to a plant expression vector for promoter analysis; a step of transforming the recombined promoter site to Agrobacteria tumefaciens strain GV3101; and a step of screening transformant.

    Abstract translation: 目的:提供甘薯叶卷曲感染DNA双向启动子,在分子生物学领域和分子育种工业中产生载体产物。 构成:甘薯叶卷曲感染DNA病毒的双向启动子由序列号1的序列表示。鉴定启动子活性的方法包括:分离具有启动子活性的位点并分析顺式作用调节位点的步骤; 通过使用网关克隆到用于启动子分析的植物表达载体来重组启动子位点的步骤; 将重组的启动子位点转化到根癌土壤杆菌菌株GV3101的步骤; 并筛选转化体的步骤。

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