국내 고구마 잎말림병 감염 DNA 바이러스의 양방향성프로모터
    5.
    发明公开
    국내 고구마 잎말림병 감염 DNA 바이러스의 양방향성프로모터 有权
    使用甜菜的双向促销蛋白

    公开(公告)号:KR1020090112492A

    公开(公告)日:2009-10-28

    申请号:KR1020080038415

    申请日:2008-04-24

    Abstract: PURPOSE: A bidirectional promoter of sweet potato leaf curl infection DNA virus is provided to produce vector product in molecular biology field and molecular breeding industry. CONSTITUTION: A bidirectional promoter of sweet potato leaf curl infection DNA virus is denoted by the sequence of the sequence number 1. A method for identifying the promoter activity comprises: a step of isolating a site with promoter activity and analyzing cis-acting regulation site; a step of recombining promoter site by using gateway cloning to a plant expression vector for promoter analysis; a step of transforming the recombined promoter site to Agrobacteria tumefaciens strain GV3101; and a step of screening transformant.

    Abstract translation: 目的:提供甘薯叶卷曲感染DNA双向启动子,在分子生物学领域和分子育种工业中产生载体产物。 构成:甘薯叶卷曲感染DNA病毒的双向启动子由序列号1的序列表示。鉴定启动子活性的方法包括:分离具有启动子活性的位点并分析顺式作用调节位点的步骤; 通过使用网关克隆到用于启动子分析的植物表达载体来重组启动子位点的步骤; 将重组的启动子位点转化到根癌土壤杆菌菌株GV3101的步骤; 并筛选转化体的步骤。

    벼흑조위축병 바이러스, 벼오갈병 바이러스 및벼줄무늬잎마름병 바이러스 진단용 프라이머 및 이를이용한 동시진단 방법
    10.
    发明授权
    벼흑조위축병 바이러스, 벼오갈병 바이러스 및벼줄무늬잎마름병 바이러스 진단용 프라이머 및 이를이용한 동시진단 방법 有权
    用于检测稻米黑色病毒的DWARF病毒,米饭病毒和稻瘟病毒病毒的检测方法以及使用该病毒检测同时存在的病毒的方法

    公开(公告)号:KR100801558B1

    公开(公告)日:2008-02-11

    申请号:KR1020060128735

    申请日:2006-12-15

    CPC classification number: C12Q1/70 C12Q1/6844 C12Q2521/119

    Abstract: A diagnosing primer is provided to diagnose respectively or simultaneously rice black-streaked dwarf virus(RBSDV), rice dwarf virus(RDV) and rice stripe virus(RSV) through RT-PCR species-specifically. A primer for diagnosing at least one virus selected from the group consisting of RBSDV, RDV and RSV is characterized in that it comprises a primer pair selected from the group consisting of a primer pair described as SEQ ID : NOs. 4 and 9 and a primer pair described as SEQ ID : NOs. 4 and 7; a primer pair selected from the group consisting of a primer pair described as SEQ ID : NOs. 16 and 24, a primer pair described as SEQ ID : NOs. 17 and 24, a primer pair described as SEQ ID : NOs. 18 and 23, a primer pair described as SEQ ID : NOs. 21 and 33, and a primer pair described as SEQ ID : NOs. 21 and 34; and a primer pair selected from the group consisting of a primer pair described as SEQ ID : NOs. 40 and 46 and a primer pair described as SEQ ID : NOs. 41 and 46. A method for diagnosing the virus comprises a step of subjecting a genome RNA of a virus sample as a template to RT-PCR using the primer.

    Abstract translation: 提供诊断引物,通过RT-PCR特异性分别或同时诊断水稻黑条纹矮病毒(RBSDV),水稻矮化病毒(RDV)和水稻条纹病毒(RSV)。 用于诊断选自RBSDV,RDV和RSV的至少一种病毒的引物的特征在于其包含选自下述引物对的引物对:SEQ ID NO: 4和9以及如SEQ ID NO:所示的引物对。 4和7; 选自由SEQ ID NO:所示的引物对组成的组的引物对。 16和24,如SEQ ID NO: 17和24,如SEQ ID NO:所示的引物对。 18和23,如SEQ ID NO: 21和33,以及如SEQ ID NO:所示的引物对。 21和34; 和选自由SEQ ID NO:所示的引物对组成的组的引物对。 40和46以及如SEQ ID NO:所示的引物对。 用于诊断病毒的方法包括使用引物对病毒样品的基因组RNA作为模板进行RT-PCR的步骤。

Patent Agency Ranking