公开(公告)号：KR101226647B1

公开(公告)日：2013-01-25

申请号：KR1020100065564

申请日：2010-07-07

Applicant: 서강대학교산학협력단

Inventor： 김정호 ,  고여림 ,  이정운

IPC: C12N5/02 ,  C07K14/72 ,  C12N5/0735

Abstract: 본 발명은 ERR (Estrogen Related Receptor)α를 유효성분으로 포함하는 Oct-4 전사인자의 활성 촉진용 조성물 및 ERRα를 유효성분으로 포함하는 만능성 세포의 분화 조절용 조성물에 관한 것이다. 본 발명의 ERRα 단백질은 전사인자 Oct-4의 활성을 촉진하여 Oct-4가 관여하는 만능성 세포의 분화과정을 조절하는데 이용될 수 있다.

公开(公告)号：KR1020080111185A

公开(公告)日：2008-12-23

申请号：KR1020070059229

申请日：2007-06-18

Applicant: 서강대학교산학협력단

Inventor： 김정호 ,  김솔 ,  이정운

IPC: C07K14/47 ,  C07K14/00

Abstract: A composition for regulating the transcriptional activity of hTAFII68(NTD) is provided to control the transcriptional activity of the hTAFII 68 (NTD) which is oncogene easily. A polypeptide for regulating the transcriptional activity of hTAFII68(NTD) comprises the hTAFII 68 (NTD) binding domain of the GAPDH protein of the second sequence. The hTAFII 68 (NTD) binding domain of the GAPDH protein of the second sequence promotes the transcriptional activity of the hTAFII 68 (NTD).

Abstract translation: 提供了一种用于调节hTAFII68（NTD）转录活性的组合物，用于控制致癌基因hTAFII 68（NTD）的转录活性。 用于调节hTAFII68（NTD）的转录活性的多肽包含第二序列的GAPDH蛋白的hTAFII 68（NTD）结合结构域。 第二序列的GAPDH蛋白的hTAFII 68（NTD）结合结构域促进hTAFII 68（NTD）的转录活性。

公开(公告)号：KR1020140117324A

公开(公告)日：2014-10-07

申请号：KR1020140115369

申请日：2014-09-01

Applicant: 서강대학교산학협력단

Inventor： 김정호 ,  임보배 ,  김아영

IPC: C07K19/00 ,  C12N15/85

Abstract: The present invention provides a method for screening a material for preventing or treating tumor diseases using novel transcription activating functions and transactivation functions of a TFG-TEC fusion protein, a pharmaceutical composition of the same, and an expression vector and a composition for promoting transcription activities using TFG (NTD). The TFG-TEC fusion protein of the present invention not only has the same sequence specificity as TEC, but also has more excellent transcription activation promoting functions than TEC. Moreover, the TFG (NTD) in the TFG-TEC fusion protein of the present invention serves as a strong transactivation domain, and PB1 domains and SPYGQ-rich regions of the TFG (NTD) have partial transactivation. As a result, the TFG-TEC fusion protein of the present invention promotes the expression of downstream genes (e.g. Skp2, L-Myc, SOCS2, or STAT-3) which are known to be regulated by TEC. Accordingly, the method for screening a material which inhibits the expression of a TFG-TEC fused gene or inhibits the activation of a TFG-TEC fusion protein and the pharmaceutical composition using the same can be usefully applied in preventing or treating tumor diseases.

Abstract translation: 本发明提供了使用TFG-TEC融合蛋白的新的转录激活功能和反式激活功能及其药物组合物以及用于促进转录活性的表达载体和组合物来筛选用于预防或治疗肿瘤疾病的材料的方法 使用TFG（NTD）。 本发明的TFG-TEC融合蛋白不仅具有与TEC相同的序列特异性，而且具有比TEC更优异的转录激活促进功能。 此外，本发明的TFG-TEC融合蛋白中的TFG（NTD）用作强反转录域，TFG（NTD）的PB1结构域和富含SPYGQ的区域具有部分反式激活。 结果，本发明的TFG-TEC融合蛋白促进已知由TEC调节的下游基因（例如Skp2，L-Myc，SOCS2或STAT-3）的表达。 因此，用于筛选抑制TFG-TEC融合基因表达的材料或抑制TFG-TEC融合蛋白的活化的方法以及使用其的药物组合物的方法可有效地应用于预防或治疗肿瘤疾病。

公开(公告)号：KR1020140113135A

公开(公告)日：2014-09-24

申请号：KR1020130028118

申请日：2013-03-15

Applicant: 서강대학교산학협력단

Inventor： 김정호 ,  임보배 ,  김아영

IPC: A61K38/00 ,  G01N33/68 ,  C12Q1/68 ,  G01N33/574

CPC classification number: G01N33/574 ,  C12Q1/6813 ,  C12Q1/6897 ,  G01N33/6893

Abstract: The present invention provides a method for screening a substance for preventing or treating tumor disorders using novel transcriptional activation and transactivation of the TFG-TEC fusion protein, a pharmaceutical composition thereof, and an expression vector and a composition for promoting transcriptional activity using TFG (NTD). The TFG-TEC fusion protein of the present invention has identical sequence specificity to the TEC, and more excellent ability to promote transcriptional activity. In addition, the TFG (NTD) in the TFG-TEC fusion protein of the present invention functions as a transactivation domain, and the PB1 domain and SPYGQ-rich regions of the TFG (NTD) has partial transactivation. As a result, the TFG-TEC fusion protein of the present invention promotes the expression of downstream genes (e.g., Skp2, L-Myc, SOCS2, or STAT-3), which has been known to be regulated by TEC. Thus, the method for screening a substance for inhibiting the expression of the TFG-TEC fusion protein or inhibit the activity of the TFG-TEC fusion protein and the pharmaceutical composition using the same can be useful to prevent or treat tumor disorders, diseases, or conditions.

Abstract translation: 本发明提供了使用TFG-TEC融合蛋白的新转录激活和反式激活，其药物组合物以及用TFG（NTD）促进转录活性的表达载体和组合物来筛选用于预防或治疗肿瘤病症的物质的方法 ）。 本发明的TFG-TEC融合蛋白具有与TEC相同的序列特异性，更优异的促进转录活性的能力。 此外，本发明的TFG-TEC融合蛋白中的TFG（NTD）用作反式激活结构域，TFG（NTD）的PB1结构域和富含SPYGQ的区域具有部分反式激活。 结果，本发明的TFG-TEC融合蛋白促进已知由TEC调节的下游基因（例如Skp2，L-Myc，SOCS2或STAT-3）的表达。 因此，用于筛选用于抑制TFG-TEC融合蛋白的表达或抑制TFG-TEC融合蛋白的活性的物质的方法和使用其的药物组合物可用于预防或治疗肿瘤病症，疾病或 条件。

公开(公告)号：KR101263758B1

公开(公告)日：2013-05-13

申请号：KR1020100102056

申请日：2010-10-19

Applicant: 서강대학교산학협력단

Inventor： 김정호 ,  이정운 ,  김영봉

IPC: C12N5/0735 ,  C12N5/02 ,  C12N5/071

Abstract: 본발명은유효성분으로서태반추출물을포함하는줄기세포용배지조성물로서, 상기배지조성물은줄기세포의증식을촉진하고, 줄기세포의미분화상태를유지하며, 줄기세포의분화능을유지하는것을특징으로하는줄기세포용배지조성물에관한것이다. 본발명에의해배양된줄기세포특히배아줄기세포는증식속도가증가된다. 본발명에의해배양된줄기세포특히배아줄기세포는줄기세포의고유한특성예를들어미분화상태및 분화능을그대로유지한다. 돼지태반추출물은비교적으로저가로용이하게구입할수 있기때문에, 줄기세포특히배아줄기세포를보다쉽게얻을수 있고배양할수 있는새로운접근방식을제공한다.

公开(公告)号：KR1020120040571A

公开(公告)日：2012-04-27

申请号：KR1020100102056

申请日：2010-10-19

Applicant: 서강대학교산학협력단

Inventor： 김정호 ,  이정운 ,  김영봉

IPC: C12N5/0735 ,  C12N5/02 ,  C12N5/071

Abstract: PURPOSE: A medium composition containing placental extract for culturing stem cells is provided to enhance proliferation rate of the embryonic stem cells and to maintain undifferentiation state and differentiation ability. CONSTITUTION: A composition for promoting proliferation of stem cells contains placental extract as an active ingredient. The medium composition contains placental extract as an active ingredient. The placental extract is derived from pigs. The stem cells are pluripotent stem cells or multipotent stem cells. A method for maintaining and increasing stem cells comprises a step of culturing the stem cells in a medium containing placental extract.

Abstract translation: 目的：提供含有培养干细胞的胎盘提取物的培养基组合物，以提高胚胎干细胞的增殖率，保持未分化状态和分化能力。 构成：用于促进干细胞增殖的组合物含有胎盘提取物作为活性成分。 培养基组合物含有胎盘提取物作为活性成分。 胎盘提取物来源于猪。 干细胞是多能干细胞或多能干细胞。 维持和增加干细胞的方法包括在含有胎盘提取物的培养基中培养干细胞的步骤。