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公开(公告)号：KR101024114B1
公开(公告)日：2011-03-29
申请号：KR1020090003454
申请日：2009-01-15
Applicant: 대한민국(농촌진흥청장)
IPC: G01N33/569 , C12N15/33 , C12Q1/68
Abstract: 본 발명은 완두돌출모자이크바이러스 검출용 PCR 프라이머쌍 및 이를 이용한 완두돌출모자이크바이러스 검출 방법에 관한 것으로서, 보다 상세하게는 서열번호 2 및 서열번호 5로 표시되는 프라이머쌍; 서열번호 2 및 서열번호 6으로 표시되는 프라이머쌍; 서열번호 3 및 서열번호 5로 표시되는 프라이머쌍 및 서열번호 3 및 서열번호 6으로 표시되는 프라이머쌍으로 이루어진 군에서 선택된 완두돌출모자이크바이러스 검출용 PCR 프라이머쌍 및 이를 이용하여 RT-PCR 또는 RT-PCR 및 nested PCR의 결합 방법에 의하여 완두돌출모자이크바이러스 검출하는 방법에 관한 것이다. 본 발명은 PEMV를 시료, 특히 콩과 식물인 시료에서 특이적으로 검출할 수 있는 PCR 프라이머쌍, 이를 이용하는 PEMV 검출 방법 및 PEMV 검출용 키트를 제공한다. 이러한 본 발명의 PCR 프라이머쌍, 검출 방법 및 검출용 키트는 미지의 시료에서 PEMV의 존재여부를 빠르고, 간편하며, 정확하게 확인할 수 있으므로 산업 및 검역 현장 등에서 유용하게 사용할 수 있다.
완두돌출모자이크바이러스, RT-PCR, 콩과 식물, Nested PCR, 검역-
22.发明公开
公开(公告)号：KR1020100084430A
公开(公告)日：2010-07-26
申请号：KR1020090003925
申请日：2009-01-16
Applicant: 대한민국(농촌진흥청장)
IPC: G01N33/569 , C12N15/33 , C12Q1/68 , C12N15/10
CPC classification number: C12Q1/686 , C12N15/11 , C12Q1/70 , G01N33/56983
Abstract: PURPOSE: A PCR primer pair for detecting peanut clump virus(PCV) and a method for detecting PCV using the same are provided to quickly and accurately detect PCV from unknown sample. CONSTITUTION: A PCR primer pair for detecting peanut clump virus(PCV) comprises a forward primer selected from sequence numbers 1-10 and a reverse primer selected from sequence numbers 11 to 18. The PCR primer pair for detecting PCV comprises: a primer pair of sequence numbers 1 and 17, sequence numbers 4 and 14, sequence numbers 4 and 16, sequence numbers 5 and 13, sequence numbers 6 and 15, or sequence numbers 9 and 13. A method for detecting PCV from a sample comprises: a step of isolating RNA from the sample; a step of performing RT-PCR; and a step of separating PCR product by size.
Abstract translation: 目的：提供用于检测花生病毒（PCV）的PCR引物对和使用其的PCV检测方法，以从未知样品快速准确地检测PCV。 构成：用于检测花生丛病毒（PCV）的PCR引物对包含选自序列号1-10的正向引物和选自序列号11〜18的反向引物。用于检测PCV的PCR引物对包含：引物对 序列号1和17，序列号4和14，序列号4和16，序列号5和13，序列号6和15或序列号9和13.用于从样品检测PCV的方法包括： 从样品中分离RNA; 进行RT-PCR的步骤; 以及按照大小分离PCR产物的步骤。
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23.发明公开
公开(公告)号：KR1020100084050A
公开(公告)日：2010-07-23
申请号：KR1020090003453
申请日：2009-01-15
Applicant: 대한민국(농촌진흥청장)
IPC: G01N33/569 , C12N15/33 , C12Q1/68 , C12N15/10
CPC classification number: C12Q1/70 , C12N15/11 , C12Q1/686 , G01N33/56983
Abstract: PURPOSE: A PCR primer pair for detecting cowpea chlorotic mottle virus(CCMV) and a method for detecting CCMV using the same are provided to accurately and quickly confirm the presence of CCMV in unknown sample. CONSTITUTION: A PCR primer pair for detecting cowpea chlorotic mottle virus comprises a primer pair of sequence numbers 8 and 12, sequence numbers 5 and 15, sequence numbers 2 and 18, sequence numbers 1 and 20, sequence numbers 1 and 21, and sequence numbers 1 and 23. A method for detecting CCMV from a sample; a step of isolating RNA from the sample; a step of performing RT-PCR using primer pair; and a step of separating PCR products.
Abstract translation: 目的：提供用于检测cow豆褪绿斑驳病毒（CCMV）的PCR引物对和使用该PCR引物检测CCMV的方法，以准确，快速地确认未知样品中CCMV的存在。 构成：用于检测cow豆褪绿斑驳病毒的PCR引物对包含序列号8和12，序列号5和15，序列号2和18，序列号1和20，序列号1和21以及序列号的引物对 一种用于从样本中检测CCMV的方法; 从样品中分离RNA的步骤; 使用引物对进行RT-PCR的步骤; 以及分离PCR产物的步骤。
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公开(公告)号：KR1020100083609A
公开(公告)日：2010-07-22
申请号：KR1020090003075
申请日：2009-01-14
Applicant: 대한민국(농촌진흥청장)
IPC: G01N33/569 , C12N15/33 , C12Q1/68 , C12N15/10
CPC classification number: C12Q1/70 , C12N15/11 , C12Q1/686 , G01N33/56983
Abstract: PURPOSE: A PCR test system for diagnosing potato spindle tuber viroid is provided to diagnose without non-specific reaction. CONSTITUTION: A primer for diagnosing potato spindle tuber viroid(PSTV) is a primer combination of sequence numbers 3 and 8, sequence numbers 3 and 9, sequence numbers 4 and 8, sequence numbers 4 and 9, sequence number 5 and 8, or sequence number 5 and 9. A method for diagnosing PSTV comprises: a step of isolating genome RNA of viroid sample; and a step of performing RT-PCR using the primer with the genome RNA as a template.
Abstract translation: 目的：提供用于诊断马铃薯块茎病毒的PCR检测系统，无需非特异性反应即可进行诊断。 构成：用于诊断马铃薯块茎病毒（PSTV）的引物是序列号3和8，序列号3和9，序列号4和8，序列号4和9，序列号5和8或序列的引物组合 一种用于诊断PSTV的方法包括：分离病毒样品的基因组RNA的步骤; 以基因组RNA作为模板的引物进行RT-PCR的步骤。
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公开(公告)号：KR1020100083605A
公开(公告)日：2010-07-22
申请号：KR1020090003071
申请日：2009-01-14
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/10 , C12N15/33 , C12Q1/68 , G01N33/569
CPC classification number: C12Q1/70 , C12N15/11 , C12Q1/686 , G01N33/56983
Abstract: PURPOSE: A system for PCR test for diagnosing Lychnis ring spot virus(LRSV) generated in Lychnis sp. plant is provided to accurately detect LRSV infection. CONSTITUTION: A primer for diagnosing Lychnis ring spot virus is: a primer combination of sequence numbers 1 and 10, sequence numbers 1 and 12, sequence numbers 1 and 14, sequence numbers 2 and 12, sequence numbers 2 and 14, sequence numbers 4 and 9, sequence number 4 and 10, sequence numbers 4 and 12, sequence numbers 5 and 9, sequence numbers 5 and 10, sequence numbers 6 and 9, sequence numbers 6 and 10, sequence numbers 6 and 11, sequence numbers 7 and 9, and sequence numbers 7 and 10. A method for diagnosing Lychnis ring spot virus(LRSV) comprises: a step of isolating genome RNA of virus sample; a step for performing RT-PCR using the primer; and a step of analyzing RT-PCR product.
Abstract translation: 目的：用于诊断Lychnis sp。中产生的Lychnis环斑病毒（LRSV）的PCR检测系统。 提供植物准确检测LRSV感染。 构成：用于诊断莱奇尼斯环斑病毒的引物是：序列号1和10，序列号1和12，序列号1和14，序列号2和12，序列号2和14，序列号4和序列号4的引物组合 9，序列号4和10，序列号4和12，序列号5和9，序列号5和10，序列号6和9，序列号6和10，序列号6和11，序列号7和9， 序列号7和10.用于诊断莱奇尼斯环斑病毒（LRSV）的方法包括：分离病毒样品的基因组RNA的步骤; 使用引物进行RT-PCR的步骤; 和分析RT-PCR产物的步骤。
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Patent Agency Ranking
公开(公告)号：KR1020100082975A
公开(公告)日：2010-07-21
申请号：KR1020090002301
申请日：2009-01-12
Applicant: 대한민국(농촌진흥청장) , 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: C12N15/10 , C12N15/11 , C12Q1/6851 , C12Q1/6853 , C12Q1/701
Abstract: PURPOSE: A primer set for diagnosing tobacco rattle virus(TRV) and a method for diagnosing TRV using the same are provided to accurately diagnose TRV infection from Solanaceae seed or plant. CONSTITUTION: A primer set for diagnosing TRV comprises a forward primer selected from sequence numbers 1, 2, 3, 4, 5 and 6, and reverse primer selected from sequence numbers 9, 10, 11, 12, 13, 14 and 15. The primer set for diagnosing TRV comprises a primer set of sequence number 3 and 1 and primer set of sequence numbers 1 and 14. A method for diagnosing TRV comprises: a step of isolating total RNA from a plant sample; a step of amplifying a target sequence by RT-PCR; and a step of detecting PCR product.
Abstract translation: 目的：提供用于诊断烟草拨鼠病毒（TRV）的引物组和使用其的诊断TRV的方法，以准确诊断茄科种子或植物的TRV感染。 构成：用于诊断TRV的引物组包含选自序列号1,2,3,4,5和6的正向引物，以及选自序列号9,10,11,12,13,14和15的反向引物。 用于诊断TRV的引物组包含序列号3和1的引物组和序列号1和14的引物组。诊断TRV的方法包括：从植物样品中分离总RNA的步骤; 通过RT-PCR扩增靶序列的步骤; 以及检测PCR产物的步骤。
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公开(公告)号：KR1020100082973A
公开(公告)日：2010-07-21
申请号：KR1020090002299
申请日：2009-01-12
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/10 , C12N15/11 , C12Q1/6851 , C12Q1/6853 , C12Q1/701
Abstract: PURPOSE: A primer set for diagnosing arabis mosaic virus(ArMV) and a diagnosis method are provided to accurately diagnose ArMV infection of various plant and to minimize damage by ArMV. CONSTITUTION: A primer set for diagnosing ArMV comprises a forward primer and reverse primer. The forward primer selected from sequence numbers 1, 2, 3 and 4. The reverse primer is selected from sequence numbers 5, 7, and 8. A kit for diagnosing ArMV comprises the primer set and DNA fragment of sequence number 11.
Abstract translation: 目的：提供用于诊断阿拉伯花叶病毒（ArMV）的引物组和诊断方法，以准确诊断各种植物的ArMV感染，并尽量减少ArMV的损伤。 构成：用于诊断ArMV的引物组包括正向引物和反向引物。 正向引物选自序列号1,2,3和4.反向引物选自序列号5,7和8.用于诊断ArMV的试剂盒包含引物组和序列号11的DNA片段。
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公开(公告)号：KR1020100082972A
公开(公告)日：2010-07-21
申请号：KR1020090002298
申请日：2009-01-12
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/10 , C12N15/11 , C12Q1/6851 , C12Q1/6853 , C12Q1/701
Abstract: PURPOSE: A primer set for diagnosing TBSV and PCR test system for diagnosing TBSV using the same are provided to use for testing TBSV from a plant. CONSTITUTION: A primer set for diagnosing tomato bushy stunt virus(TBSV) comprises a forward primer and reverse primer. The forward primer is selected from sequence numbers 2, 3, 4, 5 and 6. The reverse primer is selected from sequence number 8, 9, 10, 11, 12 and 13. The primer set for diagnosing TBSV is a primer set of sequence numbers 3 and 10 and sequence numbers 3 and 11. A method for diagnosing TBSV comprises: a step of isolating RNA from a plant sample; a step of amplifying by RT-PCR; and a step of detecting PCR product.
Abstract translation: 目的：用于诊断TBSV的引物组和用于诊断TBSV的PCR检测系统，用于从工厂测试TBSV。 构成：用于诊断番茄丛生特技病毒（TBSV）的引物组包括正向引物和反向引物。 正向引物选自序列号2,3,4,5和6.反向引物选自序列号8,9,10,11,12和13.用于诊断TBSV的引物组是序列的引物组 数字3和10以及序列号3和11.用于诊断TBSV的方法包括：从植物样品中分离RNA的步骤; 通过RT-PCR扩增的步骤; 以及检测PCR产物的步骤。
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公开(公告)号：KR1020100082964A
公开(公告)日：2010-07-21
申请号：KR1020090002286
申请日：2009-01-12
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12N15/10 , C12Q1/6851 , C12Q1/6853 , C12Q1/701
Abstract: PURPOSE: A primer set for diagnosing cherry leaf roll virus(CLRV) and a method for diagnosing CLRV using the same are provided to test CVRV from a plant in immigration quarantine and clinical diagnosis. CONSTITUTION: A primer set for diagnosing CLRV comprises a forward primer and reverse primer. The forward primer is selected from sequence numbers 1, 2, 5, 6, 7, 8, 9 and 10. The reverse primer is selected from sequence numbers 14, 15, 16, 17, 18, 19, 20, 21 and 22. A kit for diagnosing CLRV comprises the primer set and DNA fragment having a base sequence of sequence number 24. A method for diagnosing CLRV comprises: a step of isolating total RNA from a plant sample; a step of amplifying a target sequence by RT-PCR; and a step of detecting PCR product. The plant is cherry, berries, walnut, bean, plum, or pea.
Abstract translation: 目的：提供用于诊断樱桃叶卷病毒（CLRV）的引物组和使用其的CLRV诊断方法，用于在植物检疫和临床诊断中检测CVRV。 构成：用于诊断CLRV的引物组包括正向引物和反向引物。 正向引物选自序列号1，2，5，6，7，8，9和10.反向引物选自序列号14,15,16,17,18,19,20,21和22。 用于诊断CLRV的试剂盒包括引物组和具有序列号24的碱基序列的DNA片段。用于诊断CLRV的方法包括：从植物样品中分离总RNA的步骤; 通过RT-PCR扩增靶序列的步骤; 以及检测PCR产物的步骤。 植物是樱桃，浆果，核桃，豆，李子或豌豆。
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公开(公告)号：KR1020100039934A
公开(公告)日：2010-04-19
申请号：KR1020080098925
申请日：2008-10-09
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/6827 , C12Q2600/112
Abstract: PURPOSE: A specific primer for diagnosing onion yellow dwarf virus(OYDV) is provided to simply and economically diagnose OYDV and produce normal garlic. CONSTITUTION: A primer set for diagnosing onion yellow dwarf virus(OYDV) comprise a forward primer of sequence number 1, 3 or 4 and reverse primer of sequence number 2 or 5. A primer set for diagnosing OYDV comprises a primer set of sequence numbers 1 and 2, primer set of sequence numbers 3 and 2, or primer set of sequence numbers 4 and 5. A method for OYDV-specific diagnosis comprises: a step of isolating total RNA from a garlic sample; a step of amplifying target sequence through RT-PCR using the primer set and RNA template; and a step of detecting the amplified product.
Abstract translation: 目的：用于诊断洋葱黄矮病毒（OYDV）的特异性引物简单经济地诊断OYDV并产生正常大蒜。 构成：用于诊断洋葱黄矮病毒（OYDV）的引物组包括序列号1,3或4的正向引物和序列号为2或5的反向引物。用于诊断OYDV的引物组包含序列号1的引物组 2，序列号3和2的引物组或序列号4和5的引物组。一种用于OYDV特异性诊断的方法包括：从大蒜样品中分离总RNA的步骤; 使用引物组和RNA模板通过RT-PCR扩增靶序列的步骤; 以及检测扩增产物的步骤。
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