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公开(公告)号：KR1020130049754A
公开(公告)日：2013-05-14
申请号：KR1020120123842
申请日：2012-11-02
Applicant: 성균관대학교산학협력단
CPC classification number: C12Q1/6844 , C12Q1/04 , C12Q2527/101
Abstract: PURPOSE: A primer composition for loop-mediated isothermal amplification reaction for detecting tobacco leaf curl virus is provided to early detect the virus, and to prevent economic loss. CONSTITUTION: A primer set for loop-mediated isothermal amplification reaction for detecting tobacco leaf curl virus has sequence numbers 1-4. The prime set contains sequence numbers 17-20. The virus is selected from the group consisting of: NCBI No. HM164547, HM164541, HM164545, and HM164550 mutant strains found in Korea; and NCBI No. EF620536, NC_004641, NC_004654, AB287439, AB028604, AB055008, AB055009, and AB079689 mutant strains found in Japan. A composition contains DNA polymerase for loop-mediated isothermal amplification reaction, dNTPs, and a reaction buffer.
Abstract translation: 目的：提供用于检测烟叶卷曲病毒的环介导等温扩增反应的底物组合物，用于早期检测病毒，防止经济损失。 构成：用于检测烟草卷曲病毒的环介导等温扩增反应的引物组具有序列号1-4。 素数集合包含序号17-20。 该病毒选自在韩国发现的NCBI No.HM164547，HM164541，HM164545和HM164550突变株; 和NCBI No.EEF620536，NC_004641，NC_004654，AB287439，AB028604，AB055008，AB055009和在日本发现的AB079689突变株。 组合物含有用于环介导的等温扩增反应的DNA聚合酶，dNTP和反应缓冲液。
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公开(公告)号：KR101481245B1
公开(公告)日：2015-01-12
申请号：KR1020120156472
申请日：2012-12-28
Applicant: 성균관대학교산학협력단
Abstract: 본 발명은 스쿼시모자이크바이러스를 검출하기 위한 4개의 등온증폭반응용 프라이머 세트, 이를 포함하는 조성물, 및 상기 조성물을 이용한 스쿼시모자이크바이러스 검출방법에 관한 것이다. 본 발명의 프라이머 세트를 사용하면 등온증폭법을 이용하여 단시간 내에 전문장비 없이 효과적으로 스쿼시모자이크바이러스의 5종의 변이주를 모두 검출할 수 있다. 또한 고농도의 SYBR Green I을 이용하여 자연광하에서 육안으로 신속하게 진단할 수 있다. 따라서 호박, 멜론, 수박 등의 작물의 재배농가에 막대한 피해를 주고 있는 바이러스를 조기에 검출 가능하게 하여 보다 신속하고 효율적인 스쿼시모자이크바이러스 진단 시스템을 구축할 수 있을 것으로 기대된다.
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公开(公告)号：KR1020140086236A
公开(公告)日：2014-07-08
申请号：KR1020120156473
申请日：2012-12-28
Applicant: 성균관대학교산학협력단
CPC classification number: C12Q1/6844 , C12Q2527/101 , C12Q2563/107 , C12Q2565/125
Abstract: The present invention relates to a set consisting of four primers for an isothermal amplification reaction for detecting a watermelon mosaic virus, a composition comprising the same, and a method for detecting watermelon mosaic virus using the composition. The detecting method according to the present invention uses the isothermal amplification method to effectively detect all seven kinds of mutant strains of watermelon mosaic virus without specialized equipment in a short time. Moreover, the virus can be rapidly detected with the naked eye under natural light by using a high concentration of SYBR Green I. Therefore, the virus incurring huge damage to farms growing crops like pumpkin, melon, and watermelon can be detected early, thus the development of a more rapid and effective watermelon mosaic virus diagnosis system is expected.
Abstract translation: 本发明涉及由用于检测西瓜花叶病毒的等温扩增反应的四个引物，包含该引物的组合物以及使用该组合物检测西瓜花叶病毒的方法。 根据本发明的检测方法使用等温扩增方法在短时间内有效地检测所有七种不具有专门设备的西瓜花叶病毒突变菌株。 此外，通过使用高浓度的SYBR Green I，可以在自然光下用肉眼快速检测病毒。因此，可以及早检测到生长在南瓜，西瓜和西瓜等农作物上的巨大损害，因此， 开发更快速有效的西瓜花叶病毒诊断系统。
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24.发明公开
公开(公告)号：KR1020140049417A
公开(公告)日：2014-04-25
申请号：KR1020120115652
申请日：2012-10-17
Applicant: 성균관대학교산학협력단
CPC classification number: C12Q1/6827 , C12Q1/683 , C12Q1/686
Abstract: The present invention relates to a method for discriminating a mutant strain of Chrysanthemum chlorotic mottle viroid (CChMVd) using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The method according to the present invention is used to effectively discriminate msim34, SSHA6, Beijing, India 669541, and India 646404, which are five mutant strains of CChMVd. Therefore, Chrysanthemum infectious viroids that seriously damage Chrysanthemum farmhouses can be detected early, promptly, and accurately, thereby expected to significantly reduce a huge economic loss.
Abstract translation: 本发明涉及使用聚合酶链反应限制性片段长度多态性（PCR-RFLP）方法鉴别菊花褪绿斑点病毒（CChMVd）的突变菌株的方法。 根据本发明的方法用于有效区分作为CChMVd的五个突变株的msim34，SSHA6，北京，印度669541和印度646404。 因此，可以及早，准确，准确地发现严重损害菊花农场的菊花感染性病毒，预计会大大减轻经济损失。
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25.发明公开
公开(公告)号：KR1020130044375A
公开(公告)日：2013-05-02
申请号：KR1020110108063
申请日：2011-10-21
Applicant: 성균관대학교산학협력단
CPC classification number: C12Q1/6858 , C12N15/11
Abstract: PURPOSE: A primer set for detecting begomovirus beta satellite DNA and a method for detecting begomovirus beta satellite DNA is provided to have different DNA host plants, capable of detecting a respective primer set, thereby specifically detecting diagnosis of some begomovirus. CONSTITUTION: A primer set for detecting begomovirus beta satellite DNA is selected from a primer set of sequence number 1 and sequence number 2, a primer set of sequence number 3 and sequence number 4, a primer set of sequence number 5 and sequence number 6, and a primer set of sequence number 7 and sequence number 8. The primer set of sequence number 1 and sequence number 2 is for generally detecting begomovirus beta satellite DNA. The primer set of sequence number 3 and sequence number 4 is for detecting HYVMV(Honeysuckle yellow vein mosaic virus). The primer set of sequence number 5 and sequence number 6 is for detecting HYVV(Honeysuckle yellow vein virus). A method for detecting begomovirus beta satellite DNA comprises a step of separating total DNA from a plant sample; a step of amplifying a target sequence by using a primer set; and a step of detecting the amplified product.
Abstract translation: 目的：提供一种用于检测绒毛病毒β卫星DNA的引物组和一种检测酵母病毒β卫星DNA的方法，以具有不同的DNA宿主植物，能够检测各自的引物组，从而特异性检测某些枯叶病毒的诊断。 构成：从序列号1和序列号2的引物组，序列号3和序列号4的引物组，序列号5和序列号6的引物组中选择用于检测蛋白病毒β卫星DNA的引物组， 序列号为7和序列号8的引物组。序列号1和序列号2的引物组通常用于检测艾滋病毒β卫星DNA。 序列号3和序列号4的引物组用于检测HYVMV（金银花黄色静脉花叶病毒）。 序列号5和序列号6的引物组用于检测HYVV（金银花黄色静脉病毒）。 用于检测霉菌病毒β卫星DNA的方法包括从植物样品中分离总DNA的步骤; 通过使用引物组扩增靶序列的步骤; 以及检测扩增产物的步骤。
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Patent Agency Ranking26.发明公开
公开(公告)号：KR1020140086230A
公开(公告)日：2014-07-08
申请号：KR1020120156467
申请日：2012-12-28
Applicant: 성균관대학교산학협력단
CPC classification number: C12Q1/6844 , C12Q2527/101 , C12Q2563/107
Abstract: The present invention relates to a set of four primers for isothermal amplification reaction, a composition including the same, and a method for detecting Turnip yellow mosaic virus using the composition. The detection method according to the present invention can effectively detect all six mutants of the Turnip yellow mosaic virus without a professional device in a short time using the isothermal amplification method. In addition, the Turnip yellow mosaic virus can be rapidly diagnose with a naked eye under natural light using highly concentrated SYBR Green I. Therefore, the present invention facilitates early detection of virus which harms farms cultivating Brassicaceae crops such as cabbages and the like, thereby making it possible to rapidly and efficiently construct a system diagnosing the Turnip yellow mosaic virus.
Abstract translation: 本发明涉及一组用于等温扩增反应的四种引物，含有该引物的组合物，以及使用该组合物检测芜菁黄花叶病毒的方法。 根据本发明的检测方法可以使用等温扩增方法在短时间内有效地检测芜菁黄花叶病毒的所有六种突变体，而无需专业装置。 另外，利用高度浓缩的SYBR Green I，可以在自然光下用肉眼快速诊断芜菁黄花叶病毒。因此，本发明有助于早期检测危害养殖甘蓝科作物如白菜等的农场，从而 使得可以快速有效地构建诊断芜菁黄花叶病毒的系统。
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27.发明公开중합효소 연쇄 반응 및 제한효소 절편길이 다형성을 이용하여 토마토황화잎말림바이러스의 기주특이적 변이주를 검출 및 구별하는 방법 无效使用聚合酶链反应限制片段长度多态性检测和主要特异性菌株分离TOMATO黄叶病毒病毒的方法
公开(公告)号：KR1020140062936A
公开(公告)日：2014-05-27
申请号：KR1020120129258
申请日：2012-11-15
Applicant: 성균관대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: The present invention relates to a method for detecting and distinguishing a host-specific strain of a Tomato yellow leaf curl virus (TYLCV) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). More specifically, the present invention relates to a method for detecting and distinguishing Korea, Israel, Spain, USA, Jordan 1, Jordan 2, and Jordan 3 which are seven strains of the Tomato yellow leaf curl virus (TYLCV) from an amplified product, which is amplified using a primer set for PCR for detecting a host-specific strain of a TYLCV, using Dde I, Fau I, or Bss SI which is a restriction enzyme. By means of the method according to the present invention, the infection of the TYLCV and the strain can be effectively confirmed within a short period of time without high-priced technical equipment such as sequence analysis equipment. Accordingly, the spread of the TYLCV is domestically prevented, and the inflow of domestically non-occurred strains can be prevented in a plant quarantine step, so that a huge economical loss can be remarkably reduced.
Abstract translation: 本发明涉及使用聚合酶链反应限制性片段长度多态性（PCR-RFLP）检测和鉴别番茄黄叶卷曲病毒（TYLCV）的宿主特异性菌株的方法。 更具体地说，本发明涉及一种用于从扩增产物中检测和区分来自番茄黄叶卷曲病毒（TYLCV）的7株的韩国，以色列，西班牙，美国，约旦1号，约旦2号和约旦3号的方法， 其使用用于PCR的引物组进行扩增，用于检测TYLCV的宿主特异性菌株，使用DdeI，FauI或BssI， SI是限制酶。 通过本发明的方法，可以在短时间内有效地确认TYLCV和菌株的感染，而不需要高价位的技术设备如序列分析设备。 因此，TYLCV的扩散在国内被防止，在植物检疫步骤中可以防止国内未发生菌株的流入，从而显着降低了巨大的经济损失。
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公开(公告)号：KR1020130049757A
公开(公告)日：2013-05-14
申请号：KR1020120123845
申请日：2012-11-02
Applicant: 성균관대학교산학협력단
CPC classification number: C12Q1/6844 , C12Q1/04 , C12Q2527/101
Abstract: PURPOSE: A primer composition for loop-mediated isothermal amplification reaction for detecting sweet potato leaf curl virus, and a use thereof are provided to quickly diagnose the virus using high concentration SYBR green I with naked eye. CONSTITUTION: A primer set for loop-mediated isothermal amplification reaction for detecting sweet potato leaf curl virus has sequence numbers 1-4. The primer set has sequence numbers 16-19. The virus is selected from the group consisting of Korean mutant strains(HM754635, HM754636, HM754641, and FJ560719), Brazil mutant strains(HQ393473), USA mutant strains(HQ333142), Japanese mutant strains(AB433788), Chinese mutant strains(JF768740), Chinese mutant strains(JF736657), Spain mutant strains(EU856364), and India mutant strains(NC_013640). A primer composition for loop-mediated isothermal amplification reaction for detecting sweet potato leaf curl virus contains the primer set. The composition further contains DNA polymerase, dNTPs, and a reaction buffer.
Abstract translation: 目的：用于检测甘薯叶卷曲病毒的环介导等温扩增反应的底漆组合物，并提供其用于使用高浓度SYBR绿I I用肉眼迅速诊断病毒的用途。 构成：用于检测甘薯叶卷曲病毒的环介导等温扩增反应的引物组具有序列号1-4。 引物组序列号为16-19。 病毒选自韩国突变株（HM754635，HM754636，HM754641和FJ560719），巴西突变菌株（HQ393473），美国突变菌株（HQ333142），日本突变菌株（AB433788），中国突变菌株（JF768740） ，中国突变株（JF736657），西班牙突变株（EU856364）和印度突变株（NC_013640）。 用于检测甘薯叶卷曲病毒的环介导等温扩增反应的底漆组合物含有引物组。 该组合物还含有DNA聚合酶，dNTP和反应缓冲液。
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公开(公告)号：KR1020120043927A
公开(公告)日：2012-05-07
申请号：KR1020100105226
申请日：2010-10-27
Applicant: 성균관대학교산학협력단
CPC classification number: C12Q1/701 , C12Q1/6844
Abstract: PURPOSE: A primer set for detecting curtovirus and a detection method using the same are provided to quickly and accurately diagnose curtovirus from plant samples. CONSTITUTION: A primer set for detecting curtovirus contains a primer set of sequence numbers 1 and 2. The curtovirus includes beet curly top virus, beet mild curly top virus, beet severe curly top virus, horseradish curly top virus, pepper curly top virus, spinach curly top virus, or pepper yellow dwarf virus. A method for detecting curtovirus comprises: a step of isolating total DNA form a plant sample; a step of amplifying a target sequence by PCR; and a step of detecting amplified product.
Abstract translation: 目的：提供用于检测curtovirus的引物组和使用其的检测方法，从植物样品快速准确地诊断curtovirus。 构成：用于检测curtovirus的引物组含有序列号1和2的引物组。卷曲病毒包括甜菜卷曲病毒，甜菜轻度卷曲病毒，甜菜重卷曲病毒，辣根卷曲病毒，辣椒卷顶病毒，菠菜 卷曲顶级病毒，或胡椒黄矮星病毒。 检测曲霉病毒的方法包括：从植物样品中分离总DNA的步骤; 通过PCR扩增靶序列的步骤; 以及检测扩增产物的步骤。
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