公开(公告)号：KR101704382B1

公开(公告)日：2017-02-08

申请号：KR1020150155782

申请日：2015-11-06

Applicant: 충남대학교산학협력단

Inventor： 임용표 ,  최수련 ,  오상헌

IPC: C12Q1/68

Abstract: 본발명은배추뿌리혹병저항성품종을특이적으로판별하기위한프라이머세트및 이의용도에관한것으로, 본발명을통해배추뿌리혹병저항성품종에대해특이적판별이쉽고빠르게수행될수 있으므로, 배추의뿌리관련내병성품종육성시 효율적으로품종이나계통들의뿌리관련특성과병 저항성을평가함으로써배추의뿌리관련내병성품종육종에유용하게이용될수 있다.

公开(公告)号：KR101602536B1

公开(公告)日：2016-03-10

申请号：KR1020130137237

申请日：2013-11-12

Applicant: 충남대학교산학협력단

Inventor： 임용표 ,  박종태 ,  최수련 ,  조만현 ,  함인기 ,  김태일 ,  이은모

IPC: A01H4/00 ,  A01H1/04 ,  C12N5/04

Abstract: 본발명은 KACC98014P, KACC98016P, KACC98017P, KACC98018P 및 KACC98019P로이루어진군에서선택되는종자수탁번호로기탁된배추()인, 글루코나스튜틴(gluconasturtiin)의함량이증가된식물체및 이의육종방법에관한것이다. 본발명에따른식물체, 즉배추품종은글루코나스튜틴의함량이기존의배추에비해현저히높은바, 일상적으로다량섭취하는배추를통해글루코나스튜틴의항암효과를얻을수 있도록하는데 중요하게응용될수 있을것으로기대된다. 또한, 본발명에따른육종방법을통해글루코나스튜틴함량이크게향상된고기능성, 고부가가치의배추품종의개발이가능한바, 농가의수익창출에도크게기여할수 있을것으로기대된다.

公开(公告)号：KR101557417B1

公开(公告)日：2015-10-13

申请号：KR1020130153642

申请日：2013-12-11

Applicant: 충남대학교산학협력단

Inventor： 임용표 ,  박종태 ,  최수련 ,  조만현 ,  함인기 ,  김태일 ,  이은모

IPC: A01H5/00 ,  A01H1/02 ,  C12N15/82

Abstract: 본발명은비타민 C 함량이증가된배추품종및 이의육종방법에관한것으로, 본발명에따른배추품종은비타민 C의함량이기존의배추에비해현저히높은바, 일상적으로다량섭취하는배추를통해비타민 C의항산화효과를얻을수 있도록하는데 중요하게응용될수 있을것으로기대된다. 또한, 본발명에따른육종방법을통해비타민 C 함량이크게향상된고기능성, 고부가가치의배추품종의개발이가능한바, 농가의수익창출에도크게기여할수 있을것으로기대된다.

公开(公告)号：KR101361296B1

公开(公告)日：2014-02-12

申请号：KR1020130013058

申请日：2013-02-05

Applicant: 충남대학교산학협력단

Inventor： 임용표 ,  최수련 ,  우효나 ,  묘신양 ,  이수희 ,  선해정

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12N15/11 ,  C12Q1/6895

Abstract: The present invention relates to a primer set comprising at least one pair of primers selected from the group consisting a pair of primers of sequence number 1 and 2 and a pair of primers of sequence number 3 and 4 for detecting fusarium wilt resistance of radish and a method for selecting the fusarium wilt resistance of the radish. The primer set for detecting the fusarium wilt resistance of the radish according to the present invention indicates the difference of base sequences between the resistance and the susceptibility against the fusarium wilt, thereby being expected to be useful for isolating the variety of the radish resistant to the fusarium wilt and developing new varieties as well as stably detecting, selecting, and growing the variety of the radish resistant to the fusarium wilt unaffected by external selection conditions. Also, the primer set helps in preventing the yield loss and decrease of merchantable quality of the radish caused by the radish fusarium wilt, thereby being expected to be useful for increasing farm household income.

Abstract translation: 本发明涉及一种引物组，其包含选自序列号1和2的一对引物和序列号3和4的一对引物的至少一对引物，用于检测萝卜的枯萎病抗性 选择萝卜的枯萎病抗性的方法。 用于检测根据本发明的萝卜的镰刀菌枯萎病抗性的引物组表明抗性和对枯萎病的易感性之间的碱基序列的差异，因此预期可用于分离对 镰刀菌枯萎，开发新品种，以及稳定检测，选择和种植不受外部选择条件影响的对镰刀菌抗性的萝卜品种。 此外，引物组有助于防止萝卜镰刀菌引起的萝卜的产量损失和商品质量下降，从而有助于增加农户的收入。

公开(公告)号：KR101111563B1

公开(公告)日：2012-06-18

申请号：KR1020090090588

申请日：2009-09-24

Applicant: 충남대학교산학협력단

Inventor： 임용표 ,  김혜란 ,  최수련 ,  홍창표 ,  방재욱 ,  진민아 ,  박범석

IPC: C12N15/65 ,  C12N15/29 ,  C12N15/11

Abstract: PURPOSE: A genetic map of Brassica rapa provided to improve the quality of Brassica rapa and to identify an useful gene which is used in protecting crops from diseases. CONSTITUTION: A kit for identifying SSR marker for constructing a genetic map of Brassica rapa contains a primer pair and reagent for PCR. The reagent contains thermoresistant DNA polymerase, dNTPs, and buffer. A method for identifying the SSR marker for constructing the genetic map for Brassica rapa comprises: a step of isolating genomic DNA from Brassica rapa; a step of amplifying a target sequence by PCR; and a step of detecting the PCR product. A method for selecting SSR primer set for constructing the genetic map comprises: a step of selecting BAC clone from base sequence of BAC clone; and a step of designing SSR primer set for 1-3 genetic maps per BAC clone from flaking base sequence of SSR motif.

公开(公告)号：KR1020110032866A

公开(公告)日：2011-03-30

申请号：KR1020090090588

申请日：2009-09-24

Applicant: 충남대학교산학협력단

Inventor： 임용표 ,  김혜란 ,  최수련 ,  홍창표 ,  방재욱 ,  진민아 ,  박범석

IPC: C12N15/65 ,  C12N15/29 ,  C12N15/11

Abstract: PURPOSE: A genetic map of Brassica rapa provided to improve the quality of Brassica rapa and to identify an useful gene which is used in protecting crops from diseases. CONSTITUTION: A kit for identifying SSR marker for constructing a genetic map of Brassica rapa contains a primer pair and reagent for PCR. The reagent contains thermoresistant DNA polymerase, dNTPs, and buffer. A method for identifying the SSR marker for constructing the genetic map for Brassica rapa comprises: a step of isolating genomic DNA from Brassica rapa; a step of amplifying a target sequence by PCR; and a step of detecting the PCR product. A method for selecting SSR primer set for constructing the genetic map comprises: a step of selecting BAC clone from base sequence of BAC clone; and a step of designing SSR primer set for 1-3 genetic maps per BAC clone from flaking base sequence of SSR motif.

Abstract translation: 目的：提供芸苔属的遗传图谱，以提高芸苔属的品质，并确定用于保护作物免受疾病的有用基因。 构成：用于鉴定构建芸苔属遗传图谱的SSR标记的试剂盒含有PCR引物对和试剂。 试剂含有耐热DNA聚合酶，dNTP和缓冲液。 鉴定用于构建芸苔属芸苔遗传图谱的SSR标记物的方法包括：从芸苔属油菜中分离基因组DNA的步骤; 通过PCR扩增靶序列的步骤; 以及检测PCR产物的步骤。 一种选择用于构建遗传图谱的SSR引物组的方法，包括：从BAC克隆的碱基序列中选择BAC克隆的步骤; 以及从SSR基序的片状碱基序列设计每个BAC克隆的1-3个遗传图谱的SSR引物组的步骤。