公开(公告)号：KR1020130015748A

公开(公告)日：2013-02-14

申请号：KR1020110077924

申请日：2011-08-04

Applicant: 한국과학기술연구원

Inventor： 정영미 ,  김수현 ,  김상헌 ,  김영하

IPC: B29B9/12 ,  A61B17/58 ,  A61L27/14

CPC classification number: B29C47/004 ,  B29C47/0004 ,  B29C47/0009 ,  B29C47/0057 ,  B29C47/782 ,  B29C47/92 ,  B29C2947/92514 ,  B29C2947/9258 ,  B29C2947/92704 ,  B29K2067/043 ,  B29K2067/046 ,  B29K2867/043 ,  B29K2867/046 ,  B29K2995/0056 ,  B29K2995/006 ,  C08G63/08

Abstract: PURPOSE: A method for manufacturing a biodegradable polymer material is provided to reduce molecular weight and to enhance strength of the biodegradable polymer material. CONSTITUTION: A method for manufacturing a biodegradable polymer material comprises: a step of preparing a polylactide stereocomplex using polymers; a step of molding the stereocomplex by pressing; a step of cooling the molded stereocomplex; a step of extruding the stereocomplex. The polymer has a type D polymer or a type L polymer. A monomer forming the polymer includes cyclic ester monomer with chiral carbon.

Abstract translation: 目的：提供一种用于制造可生物降解的聚合物材料的方法，以降低分子量并提高可生物降解的聚合物材料的强度。 构成：生物可降解聚合物材料的制造方法包括：使用聚合物制备聚丙交酯立体络合物的步骤; 通过压制成型立体复合物的步骤; 冷却模制的立体络合物的步骤; 挤出立体络合物的步骤。 该聚合物具有D型聚合物或L型聚合物。 形成聚合物的单体包括具有手性碳的环状酯单体。

公开(公告)号：KR101225577B1

公开(公告)日：2013-01-24

申请号：KR1020100123925

申请日：2010-12-07

Applicant: 한국과학기술연구원

Inventor： 강진구 ,  김수현

IPC: G06F9/30 ,  G06F17/20

Abstract: 어셈블리(assembly) 언어 코드의 분석 장치는, 어셈블리 언어의 처리 수단에 대한 의존도(dependency) 규약 정보를 저장하는 저장 모듈; 하나 이상의 명령어를 포함하는 어셈블리 언어 코드가 입력되는 입력 모듈; 및 입력된 상기 어셈블리 언어 코드를 상기 의존도 규약 정보에 따라 분석하여, 상기 어셈블리 언어 코드에서 오류 또는 성능 저하 요인에 해당하는 부분을 결정하는 분석 모듈을 포함할 수 있다. 상기 어셈블리 언어 코드의 분석 장치를 이용하여 개발자는 해당 처리 수단에 최적화된 고성능의 어셈블리 언어 코드를 용이하게 작성할 수 있게 된다.

公开(公告)号：KR101094445B1

公开(公告)日：2011-12-15

申请号：KR1020090041846

申请日：2009-05-13

Applicant: 한국과학기술연구원

Inventor： 김상헌 ,  김수현 ,  정영미 ,  한민

IPC: C12N5/071 ,  C07K14/50 ,  C12N11/02 ,  C12N15/18

CPC classification number: C12N5/0068 ,  C07K14/47 ,  C07K14/50 ,  C07K14/503 ,  C07K17/14 ,  C07K2319/00 ,  C12N5/0663 ,  C12N2501/115 ,  C12N2533/50

Abstract: 본 발명은 줄기세포에 대한 접착활성을 갖는 섬유아세포 성장인자(fibroblast growth factor, FGF)의 재조합 단백질 및 이를 이용한 줄기세포의 배양방법에 관한 것으로서, 구체적으로 섬유아세포 성장인자의 아미노 말단에 폴리펩티드 링커가 융합되어 줄기세포에 대한 접착활성을 갖는 재조합 단백질, 및 상기 재조합 단백질을 폴리펩티드 링커의 아미노 말단을 이용하여 소수성 표면을 갖는 배양용기에 고정시킨 후, 상기 재조합 단백질이 고정된 배양용기에 줄기세포를 접착시켜 배양하는 것을 포함하는, 고정된 섬유아세포 성장인자(immobilized FGF)를 이용한 줄기세포의 배양방법에 관한 것이다. 본 발명에 따르면, FGF의 아미노 말단을 폴리펩티드 링커에 결합시키고 상기 폴리펩티드 링커의 아미노 말단을 자발적인 물리적 흡착에 의해 소수성 표면을 갖는 배양용기에 고정시킴으로써, FGF-폴리펩티드 링커의 재조합 단백질이 고정된 표면은 FGF의 생물학적 활성은 그대로 유지하면서, 외부로 노출된 FGF가 줄기세포와의 상호작용에 의한 세포접착을 유도하여 줄기세포를 효과적으로 배양할 수 있다. 따라서 본 발명은 줄기세포의 분화 및 증식 연구와, 조직공학 및 세포치료와 같은 재생의료 분야에 매우 유용하게 적용될 수 있다.
말토오스 결합 단백질(MBP), 섬유아세포 성장인자(FGF), 생물활성 표면, 세포접착, 줄기세포

公开(公告)号：KR1020110129842A

公开(公告)日：2011-12-02

申请号：KR1020110107237

申请日：2011-10-19

Applicant: 한국과학기술연구원

Inventor： 김상헌 ,  김수현 ,  박인수 ,  정영미

IPC: C12N5/071 ,  A61K35/12 ,  C12N11/04 ,  C12M3/00

CPC classification number: C12N5/069 ,  A61K35/28 ,  A61K35/30 ,  A61K35/44 ,  A61K35/51 ,  A61K35/545 ,  C12N2501/115 ,  C12N2506/1384 ,  C12N2513/00 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/70

Abstract: PURPOSE: A method for differentiation of stem cells into vascular cells by culturing in a three-dimensional cell cluster is provided to form hypoxic state and to efficiently induce the differentiation. CONSTITUTION: A composition for cell therapy for treating vascular diseases or wound contains a cell cluster containing vascular cells differentiated from the stem cell and culture liquid thereof as an active ingredient. The vascular diseases are cardiovascular diseases, cerebrovascular diseases, or ischemic diseases. The stem cells are adipose stem cells, mesenchymal stem cells, bone marrow stem cells, umbilical stem cells, nervous stem cells, or pluripotent stem cells. A three-dimensional cell cluster is formed in a hypoxia condition therein.

Abstract translation: 目的：提供通过在三维细胞簇中培养将干细胞分化成血管细胞的方法，以形成缺氧状态并有效诱导分化。 构成：用于治疗血管疾病或伤口的细胞治疗用组合物含有含有作为活性成分的干细胞和培养液分化的血管细胞的细胞簇。 血管疾病是心血管疾病，脑血管疾病或缺血性疾病。 干细胞是脂肪干细胞，间充质干细胞，骨髓干细胞，脐带干细胞，神经干细胞或多能干细胞。 在其中的缺氧条件下形成三维细胞簇。

公开(公告)号：KR1020110072805A

公开(公告)日：2011-06-29

申请号：KR1020090129884

申请日：2009-12-23

Applicant: 한국과학기술연구원

Inventor： 김일두 ,  김수현

IPC: B82B1/00 ,  D01D5/00

CPC classification number: D01F1/10 ,  B29C47/0014 ,  B29C47/887 ,  B82Y30/00 ,  C04B35/62231 ,  C04B35/62236 ,  C04B35/6224 ,  C04B35/6225 ,  C04B35/62259 ,  C04B35/62263 ,  C04B35/62268 ,  C04B35/6264 ,  C04B35/6265 ,  C04B35/62675 ,  C04B35/6268 ,  C04B35/62876 ,  C04B35/62889 ,  C04B35/62894 ,  C04B35/632 ,  C04B35/63424 ,  C04B35/63444 ,  C04B2235/3284 ,  C04B2235/3293 ,  C04B2235/444 ,  C04B2235/449 ,  C04B2235/5264 ,  C04B2235/5454 ,  D01F9/08 ,  Y10T428/2927 ,  Y10T428/298

Abstract: PURPOSE: A nanofiber and a manufacturing method thereof are provided to obtain a uniform shaped nanofiber by close packing nano particles of metal, metal oxide or metal mixed oxide. CONSTITUTION: The nanofiber is formed from close packed nano particles selected among 'the metal, the metal oxide, the metal mixed oxide and their mixture group'. A micro pore of an average pore diameter of 0.1-20nm is included between the nano particles. The porosity per unit volume is 0.01-10%. The manufacturing method of the nanofiber includes following steps.(i) A spinning solution is prepared by dissolving a precursor to form the metal more than one kind, the metal oxide or the metal mixed oxide and a mixture having two kinds of polymers with different molecular weight and glass transition temperature.(ii) The spinning solution is radiated and a precursor/polymer composite fiber is formed.(iii) The precursor/polymer composite fiber is heat-treated.

Abstract translation: 目的：提供纳米纤维及其制造方法，通过紧密堆积金属，金属氧化物或金属混合氧化物的纳米颗粒，得到均匀成形的纳米纤维。 构成：纳米纤维由“金属，金属氧化物，金属混合氧化物及其混合物组合”中的紧密堆积的纳米颗粒形成。 在纳米颗粒之间包含平均孔径为0.1-20nm的微孔。 每单位体积的孔隙率为0.01-10％。 纳米纤维的制造方法包括以下步骤：（i）通过将前体溶解以形成多于一种的金属，金属氧化物或金属混合氧化物和具有不同分子的两种聚合物的混合物来制备纺丝溶液 重量和玻璃化转变温度;（ii）照射纺丝溶液，形成前体/聚合物复合纤维;（iii）对前体/聚合物复合纤维进行热处理。

公开(公告)号：KR101024305B1

公开(公告)日：2011-03-29

申请号：KR1020100001170

申请日：2010-01-07

Applicant: 한국과학기술연구원

Inventor： 김수현 ,  송규원

IPC: G06F9/06 ,  G06F9/52 ,  G06F9/48

Abstract: PURPOSE: A system and a method for status synchronization are provided to transfer a currently performed task in a previous operating system to the next operating system. CONSTITUTION: A second operation module(31) is operated by being converted with a first operating module(32). When a process of the first operating module is finished, a first synchronization module(41) stores the status information of the first operating system. When a second operating module is started, a second synchronization module(42) controls the second operating system by the state information.

Abstract translation: 目的：提供用于状态同步的系统和方法，用于将先前操作系统中的当前执行的任务传送到下一个操作系统。 构成：通过用第一操作模块（32）转换来操作第二操作模块（31）。 当第一操作模块的处理完成时，第一同步模块（41）存储第一操作系统的状态信息。 当第二操作模块启动时，第二同步模块（42）通过状态信息控制第二操作系统。

公开(公告)号：KR1020110030030A

公开(公告)日：2011-03-23

申请号：KR1020090087959

申请日：2009-09-17

Applicant: 한국과학기술연구원

Inventor： 김상헌 ,  김수현 ,  정영미 ,  박인수

IPC: C12M1/18 ,  C12N5/071 ,  C12N15/09 ,  C12N15/12

CPC classification number: C12M35/08 ,  C12N5/0653 ,  C12N15/66

Abstract: PURPOSE: A method for differentiating adipose stem cells to adipocytes using heparin-binding domain(HBD) is provided to induce cell adhesion and to effectively obtain a large amount of adipocytes. CONSTITUTION: A culture container is fixed on a heparin-binding domain(HBD) with an adhesion activity of adipose stem cells. The HBD is fixed in a recombinant protein form with a polypeptide linker by physical absorption. The polypeptide linker is maltose-binding protein(MBP), hydrophobin, or hydrophobic cell penetrating peptides(CPPs). The recombinant protein has an amino acid sequence of sequence number 2.

Abstract translation: 目的：提供使用肝素结合结构域（HBD）将脂肪干细胞分化为脂肪细胞的方法，以诱导细胞粘附并有效获得大量的脂肪细胞。 构成：将培养容器固定在具有脂肪干细胞粘附活性的肝素结合结构域（HBD）上。 通过物理吸收将HBD固定为具有多肽接头的重组蛋白形式。 多肽接头是麦芽糖结合蛋白（MBP），疏水蛋白或疏水细胞穿透肽（CPPs）。 重组蛋白具有序列号2的氨基酸序列。

公开(公告)号：KR1020110005326A

公开(公告)日：2011-01-18

申请号：KR1020090062780

申请日：2009-07-10

Applicant: 한국과학기술연구원

Inventor： 홍재민 ,  김일두 ,  김수현 ,  최희덕

IPC: B05C3/18 ,  B05D1/26 ,  B05D3/00 ,  C09D4/00

CPC classification number: B05C19/04 ,  B05C9/14

Abstract: PURPOSE: A surface coating apparatus is provided to arrange and coat spherical particles and to enable the coating of large-sized monolayer or multilayer with only small amount of a mixture. CONSTITUTION: A surface coating apparatus includes upper and lower plates(10,20) and a suspension for coating which is injected between the upper and lower plates, wherein the suspension is applied to at least one side facing the upper and lower plates by moving at least one of the upper and lower plates. The suspension comprises microparticles(30), a solvent for dispersing the microparticles, and a dispersion catalyst.

Abstract translation: 目的：提供一种表面涂层装置，用于布置和涂覆球形颗粒，并且能够仅用少量混合物涂覆大尺寸单层或多层。 构成：表面涂覆装置包括上板和下板（10,20）和用于涂覆的悬浮液，其被注入上板和下板之间，其中悬浮液通过移动到上板和下板施加到面向上板和下板的至少一侧 上下板中至少有一个。 悬浮液包含微粒（30），用于分散微粒的溶剂和分散催化剂。

公开(公告)号：KR1020100051294A

公开(公告)日：2010-05-17

申请号：KR1020080110395

申请日：2008-11-07

Applicant: 한국과학기술연구원

Inventor： 김수현 ,  정영미 ,  김상헌

IPC: A61K35/32 ,  A61K47/30 ,  A61K47/42 ,  A61K47/36

CPC classification number: C12N5/0655 ,  A61K35/12 ,  A61K35/28 ,  A61K35/32 ,  A61K35/34 ,  A61K35/44 ,  A61K35/50 ,  A61L27/3817 ,  A61L27/3852 ,  A61L27/52 ,  A61L27/56 ,  A61L2430/06 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2501/15 ,  C12N2501/39 ,  C12N2533/40 ,  C12N2533/56 ,  A61K47/30 ,  A61K47/36 ,  A61K47/42

Abstract: PURPOSE: A cell colony-hydrogel-polymer supporting complex for a cartilage regeneration, and a cartilage regeneration composition containing thereof are provided to induce the regeneration of a cartilage while maintaining a constant form similar to a natural cartilage. CONSTITUTION: A cell colony-hydrogel-polymer supporting complex for a cartilage regeneration includes a cell colony uniformly dispersed inside a hydrogel matrix. The cell colony-hydrogel complex is dipped in the surface of a polymer supporting body and inserted into a pore to fill the pore. A producing method of the cell colony-hydrogel-polymer supporting complex comprises the following steps: forming the cell colony out of a cell capable of differentiating into a cartilage cell; forming the cell colony-hydrogel complex; and inserting the cell colony-hydrogel complex into a pore after inoculating the colony on the polymer supporting body.

Abstract translation: 目的：提供用于软骨再生的支持复合物的细胞集落水凝胶聚合物和含有其的软骨再生组合物，以在保持类似于天然软骨的恒定形式的同时诱导软骨的再生。 构成：用于软骨再生的支持复合物的细胞集落 - 水凝胶聚合物包括均匀分散在水凝胶基质内的细胞集落。 将细胞集落 - 水凝胶复合物浸入聚合物支撑体的表面并插入孔中以填充孔。 细胞集落 - 水凝胶 - 聚合物支持复合物的制备方法包括以下步骤：从能够分化成软骨细胞的细胞中形成细胞集落; 形成细胞集落 - 水凝胶复合物; 以及在将菌落接种在聚合物支持体上之后将细胞集落 - 水凝胶复合物插入孔中。

公开(公告)号：KR1020090120241A

公开(公告)日：2009-11-24

申请号：KR1020080046180

申请日：2008-05-19

Applicant: 한국과학기술연구원

Inventor： 김상헌 ,  김수현 ,  한민

IPC: C12N11/00 ,  C12N11/02 ,  C12N11/16

CPC classification number: C07K14/245 ,  C07K14/52 ,  C07K17/06 ,  C07K2319/24 ,  C12N11/06 ,  C12N11/08 ,  C12N11/14 ,  G01N33/54353

Abstract: PURPOSE: A fixation of physiologically active polypeptide with a maltose binding protein(MBP) is provided to maintain biologically activity of the polypeptide and fix the peptide on the hydrophobic surface. CONSTITUTION: A fixation of physiologically polypeptide comprises: a step of fusing the physiologically active polypeptide at carboxy terminal of maltose binding protein(MBP) to obtain a fusion protein; and a step of fixing the fusion protein on a hydrophobic surface by physical adsorption between amino terminal and the hydrophobic surface.

Abstract translation: 目的：提供具有麦芽糖结合蛋白（MBP）的生理活性多肽的固定以维持多肽的生物活性并将肽固定在疏水性表面上。 构成：生理多肽的固定包括：将麦芽糖结合蛋白（MBP）的羧基末端的生理活性多肽融合以获得融合蛋白的步骤; 以及通过氨基末端和疏水性表面之间的物理吸附将融合蛋白固定在疏水性表面上的步骤。