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公开(公告)号：KR101336604B1
公开(公告)日：2013-12-05
申请号：KR1020120037822
申请日：2012-04-12
Applicant: 한국해양과학기술원
Abstract: 본 발명은 수소 생산 혐기성 미생물을 함유하는 배양액이 내측에 저장되는 배양기; 배양기로부터 배양액을 배출시켜서 배양기에 다시 공급하도록 연결되고, 배양액의 순환 공급을 위한 펌프가 설치되는 순환라인; 순환라인을 따라 순환되는 배양액에 일산화탄소를 공급하는 일산화탄소공급부; 순환라인에 설치되고, 배양액에 일산화탄소가 용해되도록 시간을 지연시키기 위한 지연경로를 제공하는 튜브를 가지는 용해유도유닛; 및 용해유도유닛의 통과를 마친 배양액에서 분리되는 기체로부터 수소를 포집하는 수소포집부를 포함하도록 한 혐기성 미생물을 이용한 수소 생산 장치에 관한 것이다.
본 발명에 따르면, 수소 생산 혐기성 미생물을 함유한 배양액에 일산화탄소가 용해되기 위한 시간을 증가시키기 위한 지연경로를 집적도 높게 제공함으로써 배양액에 대한 일산화탄소의 용해도를 증대시키도록 하고, 이로 인해 수소 생산 혐기성 미생물이 수소 생산시 일산화탄소 가스를 효과적으로 이용할 수 있도록 하여 수소 생산의 효율을 높일 수 있다.-
公开(公告)号：KR1020120086605A
公开(公告)日：2012-08-03
申请号：KR1020110007945
申请日：2011-01-26
Applicant: 한국해양과학기술원 , 재단법인 경북해양바이오산업연구원
CPC classification number: C12P7/40 , C12N9/78 , C12Y305/05001
Abstract: PURPOSE: A novel nitrilase ORN and a method for preparing carboxylic acid using the same are provided to ensure substrate specificity and to be used as a material for pharmaceutical product synthesis. CONSTITUTION: A method for preparing carboxylic acid comprises: a step of preparing nitrilase ORN or a biocatalyst composition containing nitrilase ORN; and a step of reacting a nitrile substrate with nitrilase ORN or the biocatalyst composition. The nitrile substrate includes acetonitrile, acrylonitrile, adiponitrile, benzonitrile, butyronitrile, 4-chlorobenzonitrile, or mandelonitrile. Nitrilase ORN is prepared by a transformant. The transformant is prepared by transforming with a vector containing an nucleic acid molecule encoding an amino acid of sequence number 5.
Abstract translation: 目的：提供新的腈水解酶ORN和使用其制备羧酸的方法以确保底物特异性并用作药物合成的材料。 构成：制备羧酸的方法包括：制备腈水解酶ORN或含有腈水解酶ORN的生物催化剂组合物的步骤; 以及使腈底物与腈水解酶ORN或生物催化剂组合物反应的步骤。 腈底物包括乙腈，丙烯腈，己二腈，苄腈，丁腈，4-氯苄腈或扁桃腈。 硝酸酶ORN由转化体制备。 通过用含有编码序列号5的氨基酸的核酸分子的载体转化来制备转化体。
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公开(公告)号：KR101736485B1
公开(公告)日：2017-05-16
申请号：KR1020130033345
申请日：2013-03-28
Applicant: 한국해양과학기술원
CPC classification number: C12P3/00 , C12N9/0067 , C12Y112/98001
Abstract: 본발명은포름산염으로부터수소생산능이증가된써모코코스온누리우스엔에이원돌연변이체및 이를이용한수소생산방법에관한것이다. 본발명에따라돌연변이화된NA1는야생형보다포름산염을포함하는배지에서수소생산능력이증가되었고, 생장속도도야생형에비해증가하였다. 본발명에따른균주를이용하면포름산으로부터고효율로수소를생산할수 있다.
Abstract translation: 本发明涉及由甲酸盐产生的具有提高的产氢能力的Thermococcus onneriensis突变体以及使用该突变体产生氢的方法。 根据本发明，与野生型相比，突变型NA1增加了含有甲酸盐的培养基中的氢生产能力和生长速率。 使用根据本发明的菌株，可以从甲酸高效生产氢。
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25.发明授权
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Patent Agency Ranking
公开(公告)号：KR101580488B1
公开(公告)日：2015-12-28
申请号：KR1020130137344
申请日：2013-11-13
Applicant: 한국해양과학기술원
CPC classification number: C12P3/00 , C07K14/195 , C12N1/20 , C12N15/67
Abstract: 본발명은 rchA 유전자의발현이증가된것을특징으로하는수소생산능력이증가된써모코커스온누리누스 MC02(MC02, 수탁번호: KCTC12511BP) 균주를제공한다. 또한, 써모코커스속 균주의 rchA 유전자의발현을증가시키는단계를포함하는것을특징으로하는수소생산능력이증가된써모코커스속 균주제조방법을제공하고, 써모코커스온누리누스 MC02 균주를이용한수소생산방법을제공한다.
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公开(公告)号：KR1020150014424A
公开(公告)日：2015-02-06
申请号：KR1020140191152
申请日：2014-12-26
Applicant: 한국해양과학기술원
CPC classification number: A01N63/00 , C12N9/52 , C12N15/52 , Y10S435/816
Abstract: The present invention relates to a protease having an algicidal activity, a gene encoding the same, and an algicidal formulation including the same. The protease of the present invention has the excellent algicidal formulation, thereby being used in controlling of red tide in a red tide occurrence area. Provided in the present invention is a method for controlling red tide, which includes the step of treating the area where red tide occurs with the algicidal formulation. Specifically, provided is a method for controlling red tide which is characterized by comprising the steps of: producing at least one protease selected from the group consisting of sequence number 1, 7, and 27 by using a Kordia algicida OT1 strain (KCTC 11320BP); and putting an algicidal formulation which contains the produced protease as an active component into sea.
Abstract translation: 本发明涉及具有杀藻活性的蛋白酶，编码该蛋白酶的基因，以及包含其的杀藻剂。 本发明的蛋白酶具有优异的杀藻剂配方，因此用于防治红潮发生地区的红潮。 在本发明中提供了一种控制红潮的方法，其包括用杀藻剂制剂处理红潮区域的步骤。 具体地说，提供一种控制红潮的方法，其特征在于包括以下步骤：通过使用Kordia algicida OT1菌株（KCTC 11320BP）制备至少一种选自序列号1，7和27的蛋白酶; 并将含有所产生的蛋白酶作为活性成分的杀藻剂制剂放入海中。
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公开(公告)号：KR1020150009510A
公开(公告)日：2015-01-26
申请号：KR1020140191149
申请日：2014-12-26
Applicant: 한국해양과학기술원
CPC classification number: A01N63/00 , C12N9/52 , C12N15/52 , Y10S435/816
Abstract: The present invention relates to a protease of sequence number 5 having algicidal activity, a gene encoding the same, and an algicidal preparation including the same. The protease of the present invention has excellent algicidal activity, and thus can be useful in controlling red algal blooms in a red tide occurrence area. Provided in the present invention are a protease with algicidal activity having any one amino acid sequence among sequence number 1, 4 to 7, 26, and 27, and a gene encoding the protease.
Abstract translation: 本发明涉及具有杀藻活性的序列号5的蛋白酶，编码该蛋白酶的基因，以及包含其的杀藻剂制剂。 本发明的蛋白酶具有优异的杀藻活性，因此可用于防治红潮发生地区的红藻。 本发明提供具有杀菌活性的蛋白酶，其具有序列号1,4至7,26和27之间的任何一个氨基酸序列，以及编码该蛋白酶的基因。
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公开(公告)号：KR1020140118079A
公开(公告)日：2014-10-08
申请号：KR1020130033345
申请日：2013-03-28
Applicant: 한국해양과학기술원
CPC classification number: C12P3/00 , C12N9/0067 , C12Y112/98001
Abstract: The present invention relates to a Thermococcus onnurineus NA1 mutant having improved hydrogen producibility from formate and a method for producing hydrogen using the same. According to the present, the mutated Thermococcus onnurineus NA1 has improved hydrogen producibility in a medium including formate rather than a wild type, and improved growth velocity compared to the wild type. If a strain according to the present invention is used, hydrogen can be produced from formic acid at high efficiency.
Abstract translation: 本发明涉及具有从甲酸盐提高的氢生产能力的嗜热热球菌NA1突变体和使用其产生氢的方法。 根据本发明，突变的Thermococcus onnurineus NA1在包括甲酸而不是野生型的培养基中具有改善的氢生产能力，并且与野生型相比改善了生长速度。 如果使用根据本发明的菌株，则可以高效地从甲酸生产氢。
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公开(公告)号：KR1020140025686A
公开(公告)日：2014-03-05
申请号：KR1020120091634
申请日：2012-08-22
Applicant: 한국해양과학기술원
CPC classification number: C12N9/80 , C12P17/10 , C12Y305/01004
Abstract: The present invention relates to novel amidase, a gene encoding thereof, and a production method of caprolactam by using the amidase and the gene. The amidase of the present invention is capable of producing alpha-amino-epsilon-caprolactam from lysine. The alpha-amino-epsilon-caprolactam is a material for nylon and is internationally mass-produced. Therefore, the present invention provides a novel method of producing the alpha-amino-epsilon-caprolactam which is an environmentally friendly material for nylon.
Abstract translation: 本发明涉及新型酰胺酶，其编码基因，以及使用酰胺酶和基因的己内酰胺的制造方法。 本发明的酰胺酶能够从赖氨酸产生α-氨基 - 己内酰胺。 α-氨基-ε-己内酰胺是用于尼龙的材料，并且是国际上批量生产的。 因此，本发明提供了一种生产用于尼龙的环保材料的α-氨基 - ε-己内酰胺的新方法。
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