公开(公告)号：NZ591544A

公开(公告)日：2013-01-25

申请号：NZ59154409

申请日：2009-09-30

Applicant: ABBOTT LAB

Inventor： HSIEH CHUNG-MING ,  KUTSKOVA YULIYA A ,  MEMMOTT JOHN E

IPC: G01N33/53

Abstract: Disclosed is a method of screening an scFv antibody RNA display library, the method comprising the steps of: (a) providing a puromycin or analogue thereof crosslinked single chain Fv (scFv) mRNA molecule, said molecule comprising an mRNA encoding a 5' scFv and a 3' spacer sequence, which molecule is crosslinked to a single stranded nucleic acid linker, the linker comprising a puromycin, or analogue thereof, at a 3' end and a Psoralen C6 at a 5' end; (b) in vitro translating the puromycin-crosslinked scFv mRNA molecule in the presence of a label under mildly reducing conditions such that a labeled puromycin-crosslinked scFv mRNA/protein molecule is formed; (c) purifying the labeled puromycin-crosslinked scFv mRNA/protein molecule; (d) subjecting the purified labeled puromycin-crosslinked scFv mRNA/protein molecule to antigen selection with at least one antigen; and (e) recovering the purified labeled puromycin-crosslinked scFv mRNA/protein molecules using affinity based magnetic beads.

公开(公告)号：SG173705A1

公开(公告)日：2011-09-29

申请号：SG2011058773

申请日：2010-03-05

Applicant: ABBOTT LAB

Inventor： HSIEH CHUNG-MING ,  HUGUNIN MARGARET ,  MURTAZA ANWAR ,  MCRAE BRADFORD L ,  KUTSKOVA YULIYA ,  MEMMOTT JOHN E ,  PEREZ JENNIFER M ,  ZHONG SUJU ,  TARCSA EDIT ,  CLABBERS ANCA ,  WALLACE CRAIG ,  BRYANT SHAUGHN H ,  LEDDY MARY R

Abstract: Proteins that bind IL- 17 and/or IL- 17F are described along with there use in composition and methods for treating, preventing, and diagnosing IL- 17 related diseases and for detecting IL-17 in cells, tissues, samples, and compositions.

公开(公告)号：MX2011009362A

公开(公告)日：2011-09-26

申请号：MX2011009362

申请日：2010-03-05

Applicant: ABBOTT LAB

Inventor： HSIEH CHUNG-MING ,  LEDDY MARY R ,  PEREZ JENNIFER M ,  HUGUNIN MARGARET ,  MURTAZA ANWAR ,  TARCSA EDIT ,  MEMMOTT JOHN E ,  MCRAE BRADFORD L ,  KUTSKOVA YULIYA ,  ZHONG SUJU ,  CLABBERS ANCA ,  WALLACE CRAIG ,  BRYANT SHAUGHN H

IPC: A61K39/395

Abstract: Se describen proteínas que se unen a IL-17 y/o IL-17F junto con su uso en composiciones y métodos para tratar, prevenir, y diagnosticar enfermedades relacionadas con IL-17 y para detectar IL17 en células, tejidos, muestras, y composiciones.