公开(公告)号：EP1113850A4

公开(公告)日：2006-02-01

申请号：EP99945401

申请日：1999-09-01

Applicant: ADVION BIOSCIENCES INC ,  KIONIX INC

Inventor： MOON JAMES E ,  SCHULTZ GARY A ,  CORSO THOMAS N ,  DAVIS TIMOTHY J ,  GALVIN GREGORY J ,  LOWES STEPHEN

IPC: G01N27/62 ,  B01D15/08 ,  G01N30/60 ,  G01N30/72 ,  G01N37/00 ,  H01J49/04

CPC classification number: G01N30/6095 ,  G01N30/7266 ,  G01N2030/6013 ,  H01J49/0018 ,  H01J49/167 ,  Y10T436/24 ,  Y10T436/2575

公开(公告)号：EP1269518A4

公开(公告)日：2006-06-28

申请号：EP00988285

申请日：2000-12-22

Applicant: ADVION BIOSCIENCES INC

Inventor： SCHULTZ GARY A ,  CORSO THOMAS N ,  PROSSER SIMON J

IPC: G01N27/62 ,  B05B5/08 ,  G01N21/33 ,  G01N21/53 ,  G01N21/64 ,  G01N27/00 ,  G01N27/447 ,  G01N30/88 ,  G01N37/00 ,  H01J49/04 ,  H01J49/10 ,  H01J49/26

CPC classification number: H01J49/167 ,  H01J49/0018 ,  H01J49/04

Abstract: A microchip-based electrospray device, system, and method of fabrication thereof are disclosed. The electrospray device (250) includes a substrate (200) defining a channel (224) between an entrance orifice on an injection surface and an exit orifice on an ejection surface, a nozzle (232) defined by a portion recessed from the ejection surface surrounding the exit orifice, and an electric field generating source for application of an electric potential to the substrate to optimize and generate an electrospray (262). A method and system are disclosed to generate multiple electrospray plumes from a single fluid stream that provides an ion intensity as measured by a mass spectrometer that is approximately proportional to the number of electrospray plumes formed for analytes contained within the fluid. A plurality of electrospray nozzle devices (232) can be used in the form of an array of miniaturized nozzles for the purpose of generating multiple electrospray plumes (262) from multiple nozzles (232) for the same fluid stream . This invention dramatically increases the sensitivity of microchip electrospray devices (250) compared to prior disclosed systems and methods.

公开(公告)号：EP1252336A4

公开(公告)日：2005-02-09

申请号：EP01910423

申请日：2001-02-02

Applicant: ADVION BIOSCIENCES INC

Inventor： SCHULTZ GARY A ,  ZHANG SHENG ,  VAN PELT COLLEEN K

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  B01J2219/00274 ,  C12Q1/6869 ,  H01J49/165 ,  C12Q2565/627

Abstract: The present invention relates to a method of detecting single nucleotide polymorphisms by providing a target nucleic acid molecule, an oligonucleotide primer complementary to a portion of the target nucleic acid molecule, a nucleic acid polymerizing enzyme, and a plurality of types of nucleotide analogs. The target nucleic molecule, the oligonucleotide primer, the nucleic acid polymerizing enzyme, and the nucleotide analogs, each type being present in a first amount, are blended to form an extension solution where the oligonucleotide primer is hybridized to the target nucleic acid molecule to form a primed target nucleic acid molecules and the nucleic acid polymerizing enzyme is positioned to add nucleotide analogs to the prime target nucleic acid molecule at an active site. The oligonucleotide primer in the extension solution is extended by using the nucleic acid polymerizing enzyme to add a nucleotide analog to the oligonucleotide primer at the active site. This forms an extended oligonucleotide primer, wherein the nucleotide analog being added is complementary to the nucleotide of the target nucleic acid molecule at the active site. The amounts of each type of the nucleotide analogs in the extension solution after the extending step are then determined where each type is present in a second amount. The first and second amounts of each type of the nucleotide analog are compared. The type of nucleotide analog where the first and second amounts differ as the nucleotide added to the oligonucleotide primer at the active site is then identified. The steps of extending, determining the amounts of each type of the nucleotide analog, comparing the first and second amounts of the nucleotide analog, and said identifying the type of nucleotide analog added may be repeated, either after repeating the blending with the extended oligonucleotide primer or after determining the amounts of each type of dideoxynucleotide or dideoxynucleotide analog remaining in the extension solution as the new first amount. As a result, the nucleotide at the active site of the target nucleic acid molecule is determined. Also disclosed is an apparatus and composition for carrying out this method.