公开(公告)号：NO20024169D0

公开(公告)日：2002-09-02

申请号：NO20024169

申请日：2002-09-02

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19 ,  C12N

Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).

公开(公告)号：DE10010149A1

公开(公告)日：2001-09-06

申请号：DE10010149

申请日：2000-03-03

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19 ,  C12N9/00 ,  C07K14/00 ,  C07H21/00 ,  C12P21/02 ,  C12P7/42 ,  C12P17/00 ,  C12N15/11 ,  C12N1/20

Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).

公开(公告)号：NO20011912L

公开(公告)日：2001-04-18

申请号：NO20011912

申请日：2001-04-18

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C07H21/00 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P7/42 ,  C12N15/09 ,  C12P41/00 ,  C12R1/05 ,  C12N5/10

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：CA2347521A1

公开(公告)日：2000-04-27

申请号：CA2347521

申请日：1999-10-13

Applicant: BASF AG

Inventor： RESS-LOSCHKE MARION ,  HAUER BERNHARD ,  ENGELS DIRK ,  FRIEDRICH THOMAS ,  MATTES RALF

IPC: C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05 ,  C12N15/63

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide with nitrilase activity, to nucleic acid constructs containing the nucleic acid sequences, and to vectors containing the nucleic acid sequences or the nucleic acid constructs. The invention also relates to amino acid sequences which are coded by the nucleic acid sequences, and to microorganisms containing the nucleic acid sequences, the nucleic acid constructs or vector s containing the nucleic acid sequences or the nucleic acid constructs. In addition, the invention relates to a method for producing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：ES2280125T3

公开(公告)日：2007-09-01

申请号：ES99952558

申请日：1999-10-13

Applicant: BASF AG

Inventor： RESS-LOSCHKE MARION ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N9/78 ,  C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N15/55 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

Abstract: Una secuencia aislada de ácido nucleico que codifica para un polipéptido que tiene una actividad de nitrilasa, seleccionada del grupo de a) una secuencia de ácido nucleico que tiene representada en la SEQ ID NO: 1, b) secuencias de ácido nucleico que se derivan de la secuencia de ácido representada en la SEQ ID NO: 1 como un resultado de la degeneración del código genético. c) homólogos de la secuencia de ácido nucleico representada en la SEQ ID NO: 1, que codifican para polipéptidos que tienen al menos 98% de homología, por el rango entero de secuencia, a SEQ ID NO: 2, sin reducción en la acción enzimática de los polipéptidos.

公开(公告)号：PT1123386E

公开(公告)日：2007-03-30

申请号：PT99952558

申请日：1999-10-13

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  FRIEDRICH THOMAS ,  RESS-LOESCHKE MARION ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N9/78 ,  C07H21/00 ,  C12N15/09 ,  C12N1/21 ,  C12N15/55 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

公开(公告)号：DE59914102D1

公开(公告)日：2007-02-08

申请号：DE59914102

申请日：1999-10-13

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N9/78 ,  C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N15/55 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：AU2001233802B2

公开(公告)日：2006-01-12

申请号：AU2001233802

申请日：2001-02-27

Applicant: BASF AG

Inventor： MATTES RALF ,  RESS-LOSCHKE MARION ,  ENGELS DIRK ,  HAUER BERNHARD

IPC: C12N9/78 ,  C12N15/09 ,  C12N1/21 ,  C12N15/55 ,  C12N15/70 ,  C12N15/76 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19

Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).

公开(公告)号：PL359496A1

公开(公告)日：2004-08-23

申请号：PL35949601

申请日：2001-02-27

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19 ,  A61P41/00

Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).

公开(公告)号：ZA200207902B

公开(公告)日：2004-03-29

申请号：ZA200207902

申请日：2002-10-02

Applicant: BASF AG

Inventor： ENGELS DIRK ,  HAUER BERNHARD ,  MATTES RALF ,  RESS-LOESCHKE MARION

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19 ,  C12N ,  C12P

Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).