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    5.
    发明专利
    未知

    公开(公告)号：NO327857B1

    公开(公告)日：2009-10-05

    申请号：NO20011912

    申请日：2001-04-18

    Applicant: BASF AG

    Inventor： MATTES RALF ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  RESS-LOSCHKE MARION ,  ENGELS DIRK

    IPC: C07H21/00 ,  C12N9/78 ,  C12N1/21 ,  C12N15/09 ,  C12N15/55 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

    Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

    Nitrilase from rhodococcus rhodochrous ncimb 11216
    6.
    发明专利
    Nitrilase from rhodococcus rhodochrous ncimb 11216 未知

    公开(公告)号：AU3380201A

    公开(公告)日：2001-09-12

    申请号：AU3380201

    申请日：2001-02-27

    Applicant: BASF AG

    Inventor： RESS-LOSCHKE MARION ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

    IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19 ,  C12N15/70 ,  C12N15/76

    Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).

    Nitrilase from rhodococcus rhodochrous ncimb 11216
    8.
    发明专利
    Nitrilase from rhodococcus rhodochrous ncimb 11216 未知

    公开(公告)号：AU2001233802B2

    公开(公告)日：2006-01-12

    申请号：AU2001233802

    申请日：2001-02-27

    Applicant: BASF AG

    Inventor： MATTES RALF ,  RESS-LOSCHKE MARION ,  ENGELS DIRK ,  HAUER BERNHARD

    IPC: C12N9/78 ,  C12N15/09 ,  C12N1/21 ,  C12N15/55 ,  C12N15/70 ,  C12N15/76 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19

    Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).

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