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公开(公告)号：NO20020350D0
公开(公告)日：2002-01-23
申请号：NO20020350
申请日：2002-01-23
Applicant: BASF AG
Inventor： HAUER BERNHARD , PLEISS JUERGEN , SCHWANEBERG ULRICH , SCHMITT JUTTA , FISCHER MARKUS , SCHMID ROLF , LI QING-SHAN
IPC: C12N1/21 , C12M1/40 , C12N9/02 , C12N15/09 , C12P7/02 , C12P7/04 , C12P7/22 , C12P7/42 , C12P7/64 , C12P17/10 , C12P17/16 , C12Q1/26 , C12R1/11 , C12R1/19 , C12N
Abstract: The present invention relates to modified cytochrome P450 monooxygenases with an altered substrate profile, to nucleic acid sequences coding therefor, to expression constructs and vectors, to recombinant microorganisms which comprise these vectors, and to processes for the microbiological production of terminally or subterminally hydroxylated aliphatic carboxylic acids.
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公开(公告)号：DE10011723A1
公开(公告)日：2001-09-13
申请号：DE10011723
申请日：2000-03-10
Applicant: BASF AG
Inventor： HAUER BERNHARD , PLEIS JUERGEN , SCHWANEBERG ULRICH , SCHMITT JUTTA , FISCHER MARKUS , SCHMID ROLF , LI QING-SHAN
Abstract: An electron donor system (M1) that transfers electrons to an enzyme with redox properties comprising an inorganic, not electrode-bound, electron sink and a mediator which enable the electron transfer is new. Independent claims are also included for: (1) transferring (M2) oxygen to a hydrocarbon containing hydrogen donor molecule, where the hydrogen donor molecule is in a reaction medium comprising the oxygen transferring enzyme and (M1) in the presence of oxygen and incubating under suitable reaction conditions; (2) the enzymatic production (M3) of terminally or subterminally hydroxylated (position w-1 to w-4) fatty acids comprising: (i) mixing a hydroxylatable fatty acid or fatty acid derivative in the presence of (M1) and cytochrome P450 monooxygenase; and (ii) isolating the hydroxylated product; (3) a bioreactor useful for the production of w-hydroxylated fatty acids as described in (2); and (4) detecting (M4) fatty acid-monooxygenases comprising: (i) contacting the analyte with a w-hydroxylatable fatty acid or a derivative comprising a terminal chromophore or fluorescent label in (M1); and (ii) qualitatively or quantitatively detecting the signal.
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Patent Agency Ranking
公开(公告)号：CA2380186C
公开(公告)日：2012-03-13
申请号：CA2380186
申请日：2000-07-27
Applicant: BASF AG
Inventor： HAUER BERNHARD , PLEISS JUERGEN , SCHWANEBERG ULRICH , SCHMITT JUTTA , FISCHER MARKUS , SCHMID ROLF , LI QING-SHAN , LUTZ-WAHL SABINE , APPEL DANIEL
IPC: C12N15/09 , C12N15/53 , C12M1/40 , C12N1/21 , C12N9/02 , C12N15/70 , C12P7/02 , C12P7/04 , C12P7/22 , C12P7/64 , C12P17/10 , C12P17/16 , C12Q1/26 , C12R1/19
Abstract: The invention relates to novel cytochrome P450 monooxygenases comprising a modified substrate specificity, to nucleotide sequences which code therefor, to expression constructs and vectors containing these sequences, and to microorganisms transformed therewith. The invention also relates to methods for microbiologically oxidizing different organic substrates, such as methods for producing indigo and indirubin.
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公开(公告)号：NO20020380L
公开(公告)日：2002-03-22
申请号：NO20020380
申请日：2002-01-24
Applicant: BASF AG
Inventor： HAUER BERNHARD , PLEISS JUERGEN , SCHWANNEBERG ULRICH , SCHMITT JUTTA , FISCHER MARKUS , SCHMID ROLF D , LI QING-SHAN , LUTZ-WAHL SABINE , APPEL DANIEL
IPC: C12N15/09 , C12M1/40 , C12N1/21 , C12N9/02 , C12N15/53 , C12P7/02 , C12P7/04 , C12P7/22 , C12P7/64 , C12P17/10 , C12P17/16 , C12Q1/26 , C12R1/19 , C12N
Abstract: The invention relates to novel cytochrome P450 monooxygenases comprising a modified substrate specificity, to nucleotide sequences which code therefor, to expression constructs and vectors containing these sequences, and to microorganisms transformed therewith. The invention also relates to methods for microbiologically oxidizing different organic substrates, such as methods for producing indigo and indirubin.
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公开(公告)号：NO20020380D0
公开(公告)日：2002-01-24
申请号：NO20020380
申请日：2002-01-24
Applicant: BASF AG
Inventor： HAUER BERNHARD , PLEISS JUERGEN , SCHWANNEBERG ULRICH , SCHMITT JUTTA , FISCHER MARKUS , SCHMID ROLF D , LI QING-SHAN , LUTZ-WAHL SABINE , APPEL DANIEL
IPC: C12N15/09 , C12M1/40 , C12N1/21 , C12N9/02 , C12N15/53 , C12P7/02 , C12P7/04 , C12P7/22 , C12P7/64 , C12P17/10 , C12P17/16 , C12Q1/26 , C12R1/19 , C12N
Abstract: The invention relates to novel cytochrome P450 monooxygenases comprising a modified substrate specificity, to nucleotide sequences which code therefor, to expression constructs and vectors containing these sequences, and to microorganisms transformed therewith. The invention also relates to methods for microbiologically oxidizing different organic substrates, such as methods for producing indigo and indirubin.
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公开(公告)号：DE19955605A1
公开(公告)日：2001-05-23
申请号：DE19955605
申请日：1999-11-18
Applicant: BASF AG
Inventor： HAUER BERNHARD , PLEISS JUERGEN , SCHWANEBERG ULRICH , SCHMITT JUTTA , FISCHER MARKUS , SCHMID ROLF , LI QUING-SHAN
Abstract: Cytochrome P450 monooxygenase (I) that can oxidize at least one of the following types of substrates(A): (i) optionally substituted mono- or poly-cyclic aromatic heterocyclics containing nitrogen, sulfur or oxygen; (ii) optionally substituted mono- or poly-cyclic aromatics; (iii) linear or branched alkanes or alkenes, or (iv) optionally substituted cycloalkanes or cycloalkenes, is new.
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公开(公告)号：AU6830700A
公开(公告)日：2001-02-13
申请号：AU6830700
申请日：2000-07-27
Applicant: BASF AG
Inventor： HAUER BERNHARD , PLEISS JUERGEN , SCHWANEBERG ULRICH , SCHMITT JUTTA , FISCHER MARKUS , SCHMID ROLF , LI QING-SHAN
IPC: C12N15/09 , C12M1/40 , C12N1/21 , C12N9/02 , C12N15/53 , C12P7/02 , C12P7/04 , C12P7/22 , C12P7/64 , C12P17/10 , C12P17/16 , C12Q1/26 , C12R1/19 , C12N15/70
Abstract: The invention relates to novel cytochrome P450 monooxygenases comprising a modified substrate specificity, to nucleotide sequences which code therefor, to expression constructs and vectors containing these sequences, and to microorganisms transformed therewith. The invention also relates to methods for microbiologically oxidizing different organic substrates, such as methods for producing indigo and indirubin.
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