公开(公告)号：EP1799845A1

公开(公告)日：2007-06-27

申请号：EP04793448.4

申请日：2004-10-08

Applicant: Korea Basic Science Institute

Inventor： AHN, Yeong Hee ,  YOO, Jong Shin ,  LEE, Jae Yong ,  KIM, Jin Young ,  0HO, Kun

IPC: C12Q1/37

CPC classification number: G01N33/6842 ,  G01N33/6848

Abstract: The present invention relates to a method for phosphorylation site-specific labeling of phosphoproteome with a site-specific tagging reagent and analyzing of the resulting labeled one, more especially, a method for in-situ tagging of phosphorylation sites of phosphoproteins retained in polymeric gel with a nucleophilic tagging reagent. It also relates a method for generating new proteolytic cleavable sites at formerly phosphorylation sites by a proper choice of a nucleophilic tagging reagent. It also relates to a method for phosphopeptides analysis and phosphorylation site identification by in-gel digestion of the previously in-gel tagged proteins and subsequent mass analysis of the resulting peptides. The invention provides in-gel chemical tagging method for phosphoaminoacid residue of phosphoproteins retained in polymeric gel matrix. Phosphoprotein can be immobilized into gel matrix by a variety of methods such as gel electrophoresis. The immobilized phosphoproteins are retained in gel matrix during tagging reaction to phosphorylated aminoacid residue of phosphoproteins, and the resulting tagged proteins are also retained in gel matrix till following purification steps like washing of the tagging reagents are accomplished. The tagged proteins is digested by protease, and the resulting digested peptides is released from gel into solution and applied for peptide mass analysis.

公开(公告)号：EP2738554B1

公开(公告)日：2015-09-30

申请号：EP13814404.3

申请日：2013-01-22

Applicant: Korea Basic Science Institute

Inventor： PARK, Gun Wook ,  YOO, Jong Shin ,  KIM, Jin Young ,  LEE, Ju Yeon ,  LEE, Hyun Kyoung ,  AN, Hyun Joo ,  KIM, Jae-Han

IPC: G01N30/72 ,  G01N33/68 ,  C12Q1/34 ,  G06F17/10 ,  G06F19/00

CPC classification number: G06F19/16 ,  C12Q1/37 ,  G01N30/86 ,  G01N33/6848 ,  G01N2030/8813 ,  G01N2030/8831 ,  G01N2400/00 ,  G01N2440/38 ,  G01N2560/00 ,  G01N2570/00 ,  H01J49/0027 ,  H01J49/0036 ,  H01J49/004

公开(公告)号：EP1946119B1

公开(公告)日：2010-10-27

申请号：EP05808964.0

申请日：2005-09-14

Applicant: Korea Basic Science Institute

Inventor： AHN, Yeong Hee ,  KWON, Kyung-Hoon ,  YOO, Jong Shin

IPC: G01N33/68

CPC classification number: C07K14/4732

Abstract: The present invention relates to a method for analyzing modified polypeptide sequence and modified information thereof. More precisely, the invention relates to a method for identifying modified peptide in which fragment ion signals are obtained from target peptide precursor by using tandem mass spectrometry, candidate peptides and every possible peptide fragmentation patterns thereof are designed based on the mass data thereby, match scores corresponding to each fragmentation pattern of candidate peptides are given, and then by combining each matching scores derived from possible fragmentation patterns of a candidate peptide, modified target polypeptide is identified by tandem mass data based on the provided match score. The scoring method for modified polypeptide of the present invention can greatly contribute to increasing searching efficiency of modified peptides and reliability of the searching results by integrating all scored values obtained from different fragmentation patterns of a candidate peptide.

公开(公告)号：EP1799845B1

公开(公告)日：2010-09-15

申请号：EP04793448.4

申请日：2004-10-08

Applicant: Korea Basic Science Institute

Inventor： AHN, Yeong Hee ,  YOO, Jong Shin ,  LEE, Jae Yong ,  KIM, Jin Young ,  0HO, Kun

IPC: G01N33/68 ,  C12Q1/37

CPC classification number: G01N33/6842 ,  G01N33/6848

公开(公告)号：EP1654267B1

公开(公告)日：2010-04-21

申请号：EP03818122.8

申请日：2003-08-14

Applicant: Korea Basic Science Institute

Inventor： AHN, Yeong Hee ,  YOO, Jong Shin ,  KIM, Soohyun

IPC: C07H1/00

CPC classification number: C07H1/00